Papers - AKASHI Ryo
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バヒアグラス(Paspalum notatum Flugge)における効率的な種子由来エンブリオジェニックカルス誘導法とパーティクルガンによる形質転換条件の検討
共著者:権藤崇祐,石井由紀子,明石 良,川村修
日本草地学会誌 49: 33-37 2003.4
Language:English Publishing type:Research paper (scientific journal)
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Fundamental studies on the improvement of some warm-season grasses by means of plant biotechnology.
Akashi R, Gondo T, Kawamura O
Jpn J Grassl Sci 49 79 - 87 2003.4
Language:Japanese Publishing type:Research paper (scientific journal)
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バヒアグラス(Paspalum notatum Flugge)の単一種子由来懸濁培養細胞からの再分化個体における形態的および飼料的性状の変異 Reviewed
サレン カマルディン, 那須 幹夫, 新美 光弘, 明石 良, 川村 修
日本草地学会誌 49 ( 5 ) 456 - 459 2003
Language:English Publishing type:Research paper (scientific journal) Publisher:日本草地学会
バヒアグラス(品種ペンサコラ)の単一種子由来懸濁培養細胞から再分化した個休における外部形態, 細胞壁構成物質含量およびルーメン微生物による in vitro 消化率を調査した結果, これらの性状に変異が認められた。全ての再分化個休のNDF含量は, 種子からの植物休(対照個休)のそれより高かった。再分化個休の消化率は草丈と有意な負の相関があった。これらの結果は, 組織培養が暖地型牧草の品質改変のための育種操作として有用であることを示唆している。
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RAPD(Random amplified polymorphic DNAs)分析法によるネピアグラス(Pennisetum purpureum Schumach.)の系統分類
共著者:霍田真一,明石 良,川村 修,沼口寛次
日本草地学会誌 48: 37-42 2002.8
Language:English Publishing type:Research paper (scientific journal)
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Dry matter and Lignin Distributions Determined after Micro-surgical tissue separation in stem of maize (Zea mays L.) at different maturation stages
共著者:O. Kawamura, N Niijima, M. Niimi, R. Akashi,
Grassland Science 47: 578-582 2002.4
Language:English Publishing type:Research paper (scientific journal)
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ネピアグラス(Pennisetum purpureum Schumach.)の形態的特性とブリックス値およびリグニン含量との関係
共著者:霍田真一,明石 良,川村修,沼口寛次
日本草地学会誌 47: 604-609 2002.4
Language:English Publishing type:Research paper (scientific journal)
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Bialaphos-resistant Cells of Dallisgrass (Paspalum dilatatum Poir.) through Particle Bombardment with a Simple Self-built Inflow Gun
共著者:R. Akashi, C. Yuge, T. Gondo, O. Kawamura, F. Hoffmann
Grassland Science 47: 588-593. 2002.4
Language:English Publishing type:Research paper (scientific journal)
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TSURUTA Shin-ichi, AKASHI Ryo, KAWAMURA Osamu, NUMAGUCHI Hirotsugu
Japanese Journal of Grassland Science 48 ( 1 ) 37 - 42 2002
Language:Japanese Publishing type:Research paper (scientific journal) Publisher:Japanese Society of Grassland Science
We conducted a study of genetic relationships among introduced lines of napiergrass (Pennisetum purpureum Schumach.) based on random amplified polymorphic DNAs (RAPD) analysis. Fifteen lines of napiergrass, which included one interspecific hybrid (P. americanum (L.) Leeke× P. purpureum Schum.) and one dwarf line,were used in this study. From the forty-decamer random primers used, 132 RAPD bands were scored, of which 112 (84.8%) were polymorphic. Twenty one primers resulted in no detected amplification. Three primers produced specific banding patterns that were useful in an identification of these lines. Cluster analysis based on the 132 RAPD bands revealed that the fifteen lines were classified into six groups as reflected in their origin. It was suggested that RAPD analysis could be a useful tool for detailed genetic characterization in napiergrass.
DOI: 10.14941/grass.48.37
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トウモロコシ節間における乾物およびリグニンの分布 Reviewed
川村 修, 新島 範高, 新美 光弘, 明石 良
日本草地学会誌 47 ( 6 ) 578 - 582 2002
Language:English Publishing type:Research paper (scientific journal) Publisher:日本草地学会
開花, 乳熟および完熟期におけるトウモロコシの上, 中, 下位節間を4つの組織画分に顕微解剖的に分離し分析した。節間の全乾物に占める各画分の割合は, 維管束画分の割合が最も低く, 次いで表皮, 厚壁組織, 髄画分の順であった。この値は各組織画分が横断切片上に占める面積の割合と大きく異なっていた。リグニン濃度は表皮画分が最も高く, 次いで厚壁組織, 維管束, 髄画分の順であった。上位の節間ほど髄画分のリグニン濃度は高まり, 逆に, 表皮, 厚壁組織画分では低下した。節間が含有する全リグニンのうち, 厚壁組織画分に分布するリグニンの割合は下位の節間ほど高く, 逆に, 髄画分に分布するリグニンの割合は上位の節間ほと高かった。
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Plants from protoplasts isolated from a long-term root culture (Super Root) of Lotus corniculatus. Reviewed
共著者:R. Akashi, S. Harris, S.-S Hoffmann-Tsay , F. Hoffmann
J. Plant Physiol. 157: 215-221. 2000.4
Language:English Publishing type:Research paper (scientific journal)
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Plants from protoplasts isolated from a long-term root culture (super root) of lotus corniculatus Reviewed
Akashi R., Harris S., Hoffmann-Tsay S., Hoffmann F.
Journal of Plant Physiology 157 ( 2 ) 215 - 221 2000
Language:English Publishing type:Research paper (scientific journal) Publisher:Journal of Plant Physiology
In the legume bird's-foot trefoil (Lotus corniculatus L.), a super-growing root (super root) culture has been recently introduced. This system is unique because it allows continuous root cloning, somatic embryogenesis and mass regeneration of plants under entirely hormone-free culture conditions. Here we report the isolation and culture of protoplasts from this long-term root culture (more than three years old) and the regeneration of plants from super root-derived protoplasts. Treatment of pre-cultured super roots with an enzyme mixture containing 4.0 % cellulase and 0.1% pectolyase in 0.25 mol/L mannitol/O.25mol/L sorbitol plus 0.1% CaCl 2 led to the separation of the root tips from the rest of the roots and, within four hours, to the isolation of approximately 3.0 x 10 6 protoplasts per gram root tissue. Protoplasts were released almost entirely from the separated root-tips and cultured, embedded in small agarose disks, in a modified KM8P medium supplemented with 0.05 mg/L BAP, 0.1 mg/L 2, 4-D and 0.5 mg/L NAA. After five to seven days, the first divisions were observed, and after four weeks micro calli of 0.5 to 1 mm could be recovered. Calli were placed on MS medium containing relatively high concentrations of BAP and NAA (both 1.5 mg/L) for four weeks, resulting in prolific shoot formation. Elongated shoots rooted easily on hormone-free medium, and plants could be established. Roots from regenerated plants and roots formed directly on protoplast-derived calli were used to establish new root cultures. These newly initiated cultures expressed all the super root qualities again, including prolific shoot regeneration upon transfer to light, indicating that the super-growing character is not lost through protoplast isolation and regeneration. The super root pathway of regeneration, from protoplasts to callus, roots, root culture and plants, allows the virtually unlimited mass regeneration of plants from root protoptasts. Regenerating super root protoplasts add an important component to tissue culture systems for legume/Rhizobium research. Furthermore, this is the first report on plant regeneration from protoplasts isolated from a true root culture rather than excised roots.
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Improvement of forage quality in bahiagrass (Paspalum notatum Flugge) through genetic manipulation. Detection of somaclonal variation using RAPD analysis in plants regenerated from suspension cultures
共著者:R. Akashi, O. Kawamura
Grassland Science 44: 203-207 1998.10
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Selection of a super-growing legume root culture that permits controlled switching between root cloning and direct embryogenesis
共著者:R. Akashi, S.-S Hoffmann-Tsay, F. Hoffmann
Theoretical and Applied Genetics 96: 758-764 1998.5
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High-frequency embryo-genesis from cotyledons of bird's-foot trefoil (Lotus corniculatus) and its effective utilization in Agrobacterium tumefaciens-mediated transformation
共著者:R. Akashi, T. Uchiyama, A. Sakamoto, O. Kawamura, F. Hoffmann
Journal of Plant Physiology 152: 84-91 1998.3
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遺伝子操作によるバヒアグラスの品質向上 : 1. RAPD分析による懸濁培養細胞由来再生個体におけるソマクローナル変異の検出 Reviewed
明石 良, 川村 修
日本草地学会誌 44 ( 3 ) 203 - 207 1998
Language:English Publishing type:Research paper (scientific journal) Publisher:日本草地学会
バヒアグラスの懸濁培養細胞由来再生個体におけるソマクローナル変異をRAPD分析を用いて調査した。分析に用いた個体は, 発芽種子由来5個体(Control個体)と再生個体のうち任意に選んだ28個体である。その結果, 任意の配列を持つ10塩基のプライマー10種類のうち, 1種類のプライマ-(OPA-06)において2再生個体でControl個体と異なる多型を示し, 1つのバンドが欠失していることが確認された。
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Internal zinc accumulation is correlated with increased growth in rice suspension culture
共著者:B. Hossain, N. Hirata, R. Akashi, Y. Nagatomo, H. Takaki
Journal of Plant Growth Regulation 16: 239-243 1997.10
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チモシー (Phleum pratense L.) の幼穂からのカルス形成と植物体再分化
共著者:明石 良,池田一
日本草地学会誌 33: 291-292 1997.3
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アポミクシスギニアグラスの懸濁培養細胞由来プロトプラストからのエンブリオジェニックカルスの形成 Reviewed
明石 良, LACHMANN Siegfried, HOFFMANN Franz, 足立 泰二
Breeding science 45 ( 4 ) 445 - 448 1995.12
Language:English Publishing type:Research paper (scientific journal) Publisher:日本育種学会
ギニアグラスは,西南暖地において重要な暖地型牧草として知られている.しかしながら,ギニアグラスの生殖様式はアポミクシスであるため,遺伝的変異が少なく,交配育種を進める上で大きな障害となっている.著者らはギニアグラスの変異拡大を目的として,体細胞変異選抜や外来遺伝子の導入などの遺伝子工学技術や細胞融合法などの細胞工学的手法を用いて品種改良を試みている.そこで,本報はこれら技術の基礎となるプロトプラスト培養法を検討した.先の報告(Akashi and Adachi,1991)から体細胞不定胚形成および植物体の再生能の高い品種"ペトリ''のカルスを用いて,1.Omg/12,4-D添加のMS培地で懸濁培養を行い,培養開始6ヵ月以降の懸濁細胞をプロトプラストの材料とした.
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Embryogenic callus formation from protoplasts derived from suspension cells of apomictic guineagrass (Panicum maximum Jacq.)
共著者:R. Akashi, S. Lachmann, F. Hoffmann, T. Adachi
Breeding Science 45: 445-448 1995.12
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Plant regeneration from seed-derived embryogenic callus and cell suspension cultures of bahiagrass (Paspalum notatum)
共著者:R. Akashi, A. Hashimoto, T. Adachi
Plant Science 90: 73-80 1993.12