Papers - SUZUKI Yoshihiro
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Temporal variability in genetic patterns and antibiotic resistance profiles of enterococci isolated from human faeces Reviewed
Nishiyama M, Shimauchi H, Suzuki Y
Microbes and Environments 31 ( 2 ) 182 - 185 2016.7
Language：English Publishing type：Research paper (scientific journal)
Temporal variabilities in the genetic patterns and antibiotic resistance profiles of enterococci were monitored over a 7-month
period. Enterococcus faecalis isolates (103 strains) collected from feces showed only one genetic pattern and antibiotic resistance
profile within 0 d and 30 d. In contrast, after 60 d and 90 d, the genetic patterns and antibiotic resistance profiles of all E.
faecalis isolates (8 strains) clearly differed within 30 d. These results indicate that the genetic patterns and antibiotic resistance
profiles of E. faecalis in human feces changed to completely dissimilar patterns between 1 and 2 months. -
Concentration of MS2 Phage in River Water by a Combined Ferric Colloid Adsorption and Foam Separation-Based Method, with Ms2 Phage Leaching from Ferric Colloid Reviewed
Suzuki Y, Kobayashi T, Nishiyama M, Kono T
Journal of Bioscience and Bioengineering 122 ( 2 ) 252 - 256 2016.6
Language：English Publishing type：Research paper (scientific journal)
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ナイルデルタの農業排水路における水質汚濁の実態調査 Reviewed
小木曽凡芳, 大石貴行, 鈴木祥広
環境技術 45 ( 4 ) 206 - 213 2016.4
Language：Japanese Publishing type：Research paper (scientific journal)
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TRACING THE SOURCE OF SEDIMENT IN HITOTSUSE DAM RESERVOIR USING MINERAL ANALYSIS Reviewed
ARAO Yasuhiro, ITOU Kenichi, OISHI Hiroyuki, MURAKAMI Toshiki, SUZUKI Yoshihiro
Journal of Japan Society of Civil Engineers, Ser. G (Environmental Research) 72 ( 2 ) 12 - 23 2016.4
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japan Society of Civil Engineers
Sediments have become a serious problem in the management of the Hitotsuse Dam Reservoir (reservoir capacity: 261,361,500 m3), Miyazaki, Japan. The prevention of sediments in reservoirs remains a challenge, because the catchments can contain many landslip areas and the strata architecture is complex. In this study, we investigate the source of the sediments in the reservoir using mineral analysis. The core sample was collected by a boring machine from a 16 m sediment depth. To assess the nature of the particles, landslide soils accumulated in catchments of the Hitotsuse Dam were sampled and analyzed. Energy dispersive X-ray fluorescence (ED-XRF) and X-ray diffraction (XRD) were used to determine the component composition and mineral composition of the sediments, respectively. Cluster analyses were performed to evaluate the similarity in the component and mineral compositions of each sample. Component and mineral compositions of core samples indicated high similarities with the landslide soil to three samples out of 60 samples taken at 22 points. The points of three samples locate along the same stratum. It was strongly suggested that the sediments were continuously supplied from the same stratum located in the upper river basin. This method has the potential to determine the substances that release sediments in reservoirs and the location of the substances in the soil. Appropriate countermeasures to reduce the accumulation of sediments can then be taken at those specific locations.
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二液調質による下水汚泥脱水ケーキの低含水化に関する基礎的研究 Reviewed
鈴木祥広, 荒生靖大, 吉田在秀, 菊川哲生, 伊藤貫浩, 仲元寺宣明, 浜本洋一
用水と廃水 57 ( 9 ) 687 - 695 2015.9
Language：Japanese Publishing type：Research paper (scientific journal)
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火山灰凝集材による濁水の凝集特性 Reviewed
鈴木祥広, 玄晄植, 吉野内謙, 伊藤健一, 和田信一郎
用水と廃水 57 ( 3 ) 193 - 201 2015.3
Language：Japanese Publishing type：Research paper (scientific journal)
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Identification of Enterococcus faecium and Enterococcus faecalis as vanC-type vancomycin-resistant enterococci (VRE) from sewage and river water in the provincial city of Miyazaki, Japan Reviewed
Nishiyama T, Iguchi A, Suzuki Y.
Journal of Environmental Science and Health, Part A 50 16 - 25 2015.2
Language：English Publishing type：Research paper (scientific journal)
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NISHIYAMA Masateru, TAKESHITA Yusaku, SUZUKI Yoshihiro
Japan journal of water pollution research 38 ( 2 ) 57 - 65 2015.2
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japan Society on Water Environment
Enterococci are fecal indicator bacteria that are widely distributed in water environments such as sewage, urban rivers, and coastal areas. In addition, nosocomial infectious diseases caused by vancomycin-resistant enterococci (VRE) have arisen in medical institutions. VRE have the potential to exist not only in hospital facilities but also in environmental water sources. In this study, VRE and other antibiotic-resistant enterococci isolated from sewage and rivers in the provincial city of Miyazaki, Japan, were investigated. There were no VRE in sewage and river water samples. However, vancomycin-intermediate-resistant enterococci were observed in both samples. The percentages of vancomycin-intermediate-resistant isolates from sewage and river water were 8.4% (20/239 isolates) and 1.9% (5/261 isolates), respectively. More than 60% of isolates from sewage and river water were resistant to erythromycin and tetracycline, which are universally applicable antibiotics. The monitoring performed monthly between June 2011 and July 2012 indicated that the percentages of antibiotic-resistant enterococci fluctuated every month. Enterococci resistant to various antibiotics exist ubiquitously in the water environment.
DOI： 10.2965/jswe.38.57
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Concentration of Cryptosporidium oocysts from river water by coagulation and foam separation combined with acid dissolution of ferric flocs Reviewed
Suzuki Y, Takida T.
Journal of Environmental Science and Health, Part A 50 311 - 316 2015.1
Language：English Publishing type：Research paper (scientific journal)
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Water Quality Purification for Agriculture Drain in the Nile Delta by the Micro Bubbles
OGISO Tsuneyoshi, OISHI Takayuki, SUZUKI Yoshihiro
J. Env. cons. eng 44 ( 4 ) 220 - 227 2015
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Society of Environmental Conservation Engineering
DOI： 10.5956/jriet.44.220
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模擬家畜を用いた埋却実験による環境負荷物発生挙動に関する研究 Reviewed
関戸知雄, 藤原尚洋, 土手裕, 鈴木祥広, 稲垣仁根, 森田哲夫
土木学会論文集G（環境） 70 III_457 - III_467 2014.11
Language：Japanese Publishing type：Research paper (scientific journal)
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ダム貯水池における濁水長期化を引き起こす原因粒子の発生源追跡
村上俊樹, 太田勝康, 鈴木祥広電力土木
電力土木 369 ( 1 ) 37 - 41 2014.1
Language：Japanese Publishing type：Research paper (scientific journal)
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SEKITO Tomoo, FUJIWARA Naohiro, DOTE Yutaka, SUZUKI Yoshihiro, INAGAKI Hitone, MORITA Tetsuo
PROCEEDINGS OF ENVIRONMENTAL AND SANITARY ENGINEERING RESEARCH 70 ( 7 ) III_457 - III_467 2014
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japan Society of Civil Engineers
In 2010, an foot-and-mouth disease epidemic occurred in Miyazaki Prefecture, Japan. Over the period from April to August 2010, almost 290,000 animals were culled and buried. The burial of these carcasses significantly increased people's awareness of environmental contamination. However, little information has been generated on environmental pollution from burial sites. In this study, the characteristics and contamination levels of leachate and gas produced at the burial site were determined using laboratory-scale columns that simulated the burial site. In the case of higher water injection intensity, solubilization of organic substances was initiated in the early stages of the experiment. When the ammonia concentrations in the column decreased to a level suitable for methane fermentation, the amount of gas generated started to increase because of an increase in methane gas production. In the case where water was not injected, a significantly smaller amount of gas was generated except in the early stages of the experiment. The amount of methane gas generated in the column was reduced with the use of sawdust. During the two-year experimental period, the residual carbon concentration was 60%-70% and 90% in the column with water injection and the column without water injection, respectively.
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A proposal of source tracking of fecal pollution in recreational waters by pulsed-field gel electrophoresis Reviewed
T. Furukawa, Y. Suzuki
Microbes and Environments 28 ( 4 ) 444 - 449 2013.11
Language：English Publishing type：Research paper (scientific journal)
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下水の応急措置的処理を目的とした無機粘土系凝集剤による簡易凝集沈殿 Reviewed
鈴木祥広, 椎屋朋子, 西山正晃, 島内英貴, 牛島理博, 関戸知雄
用水と廃水 55 ( 11 ) 823 - 830 2013.11
Language：English Publishing type：Research paper (scientific journal)
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Electricity generation from cattle manure slurry by cassette-electrode microbial fuel cells
Inoue Kengo, Ito Toshihiro, Kawano Yoshihiro, Iguchi Atsushi, Miyahara Morio, Suzuki Yoshihiro, Watanabe Kazuya
Journal of bioscience and bioengineering 116 ( 5 ) 610 - 615 2013.11
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：公益社団法人日本生物工学会
Cassette-electrode microbial fuel cells (CE-MFCs) are efficient and scalable devices for electricity production from organic waste. Previous studies have demonstrated that CE-MFCs are capable of generating electricity from artificial wastewater at relatively high efficiencies. In this study, a single-cassette CE-MFC was constructed, and its capacity for electricity generation from cattle manure suspended in water (solid to water ratio of 1:50) was examined. The CE-MFC reactor was operated in batch mode for 49 days; electricity generation became stable 2 weeks after initiating the operation. The maximum power density was measured at 16.3 W m^<-3> on day 26. Sequencing analysis of PCR-amplified 16S rRNA gene fragments obtained from the original manure and from anode biofllms suggested that Chlorofiexi and Geobacteraceae were abundant in the anode biofllm (29% and 18%, respectively), whereas no Geobacteraceae sequences were detected in the original manure sample. The results of this study suggest that CE-MFCs can be used to generate electricity from water-suspended cattle manure in a scalable MFC system.
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Electricity generation from cattle manure slurry by cassette-electrode microbial fuel cells Reviewed
Inoue K., Ito T., Kawano Y., Iguchi A., Miyahara M., Suzuki Y., Watanabe K.
Journal of Bioscience and Bioengineering 116 ( 5 ) 610 - 615 2013.11
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Bioscience and Bioengineering
Cassette-electrode microbial fuel cells (CE-MFCs) are efficient and scalable devices for electricity production from organic waste. Previous studies have demonstrated that CE-MFCs are capable of generating electricity from artificial wastewater at relatively high efficiencies. In this study, a single-cassette CE-MFC was constructed, and its capacity for electricity generation from cattle manure suspended in water (solid to water ratio of 1:50) was examined. The CE-MFC reactor was operated in batch mode for 49 days; electricity generation became stable 2 weeks after initiating the operation. The maximum power density was measured at 16.3Wm -3 on day 26. Sequencing analysis of PCR-amplified 16S rRNA gene fragments obtained from the original manure and from anode biofilms suggested that Chloroflexi and Geobacteraceae were abundant in the anode biofilm (29% and 18%, respectively), whereas no Geobacteraceae sequences were detected in the original manure sample. The results of this study suggest that CE-MFCs can be used to generate electricity from water-suspended cattle manure in a scalable MFC system. © 2013 The Society for Biotechnology, Japan.
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置砂施工に用いるダム碓砂の洗浄工程で発生する濁水の簡易凝集処理
鈴木祥広
河川 807 ( 10 ) 49 - 53 2013.10
Language：Japanese Publishing type：Research paper (scientific journal)
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河川定点におけるふん便指標細菌の遺伝子パターンの変遷 Reviewed
古川隼士, 島内秀貴, 鈴木祥広
環境技術 42 ( 10 ) 617 - 624 2013.10
Language：Japanese Publishing type：Research paper (scientific journal)
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Suzuki Y., Kajii S., Nishiyama M., Iguchi A.
Science of the Total Environment 450-451 148 - 154 2013.4
Language：English Publishing type：Research paper (scientific journal) Publisher：Science of the Total Environment
Pseudomonas aeruginosa is responsible for a number of opportunistic and nosocomial infections. However, very little information is available on the ecology of P. aeruginosa in water environments and its association with antimicrobial resistance. In this study, the distribution of P. aeruginosa and the resistance of P. aeruginosa isolates to various antibiotics were investigated from two rivers, Kiyotake and Yae that flow through Miyazaki City, Japan. P. aeruginosa was distributed widely along the river basins with counts ranging from 2-46. cfu/100. mL. The susceptibility of P. aeruginosa isolates collected from the rivers to various antibiotics was examined by minimum inhibitory concentration. Multidrug-resistant P. aeruginosa strains were not observed or isolated from either river. However, one piperacillin-resistant P. aeruginosa was detected among a total of 516 isolates, and this isolate was also resistant to cefotaxime and showed intermediate resistance to cefitazidime. Less than 1% of all isolates (n = 5) were resistant to imipenem, which is the most effective antibiotic against both Gram-negative and Gram-positive bacteria. However, all P. aeruginosa isolates were completely resistant to tetracyclines, which are the most commonly prescribed antibiotics. In advanced nations such as Japan where the majority of the population is urban and where medical services are widespread, antibiotic-resistant bacteria such as P. aeruginosa are likely to be widely distributed, even in apparently pristine rivers. © 2013 Elsevier B.V.