Papers - SUZUKI Yoshihiro
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凝集・泡沫分離法による淡水赤潮Peridinium bipesの除去 Reviewed
仲元寺宣明，鈴木祥広，丸山俊朗
環境工学研究論文集 37 259 - 266 2000.11
Language：English Publishing type：Research paper (scientific journal)
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Phytotoxicity of the new antifouling compound Irgarol 1051 and a major dehration product Reviewed
H. Okamura, I. Aoyama, T. Takami, T. Maruyama, Y. Suzuki, M. Matsumoto, I. Katsuyama, J. Hamada, T. Beppu, O. Tanaka, R. J. Maguire, D. Liu, Y. Lau, G. J. Pacepavicus
Marine Pollution Bulletin 40 ( 9 ) 754 - 763 2000.9
Language：English Publishing type：Research paper (scientific journal)
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Removal of Suspended Matter from an Aqueous Solution by Foam Separation with Fish Mucus Reviewed
SUZUKI Yoshihiro, MARUYAMA Toshiroh
Japan journal of water pollution research 23 ( 3 ) 181 - 186 2000.3
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japan Society on Water Environment
An experimental investigation of the removal of suspended matter from an aqueous solution was carried out with a foam separation batch system using fish mucus as the chemical. Flounder and eel mucus showed a high foaming capacity. The capacity was influenced by changes in pH and by the presence of salts in the solution. The capacity of eel mucus was weaken in the weak acidic solution (pH 5), and was enhanced in the alkaline solution (pH 10). The capacity of both types of mucus were elevated with addition of NaCl. The capacity of flounder mucus was extremely high in seawater, and the height of foaming layer was 7 times higher than in distilled water at the same concentration. When the flounder mucus was added to the bubbling solution (above 3mg · <i>l</i><sup>-1</sup>), the suspended kaolin particles were adsorbed on the foam layer and were removed from the seawater by separation. The removal efficiency was 99%. The eel mucus also removed the kaolin in the presence of salts. Foam separation is a suitable method for removing suspended matter from fish rearing water that contains fish mucus.
DOI： 10.2965/jswe.23.181
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Effects of pH and Coexistent Substances on the Foam Formation of a Protein Solution Reviewed
SUZUKI Yoshihiro, MARUYAMA Toshiroh
Japan journal of water pollution research 23 ( 2 ) 108 - 115 2000.2
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japan Society on Water Environment
To establish a foam separation technique using proteins as a foaming reagent, it is important to know the foaming characteristics of various protein solutions and to demonstrate the effective application of these proteins. In this study, the effects of pH and coexistent substances on the foam formation of various proteins were examined.<br>The results are as follows.<br>(1) The foaming capacity of globular proteins such as casein, hemoglobin, soy protein, and albumin was enhanced in both strong acid and alkaline solutions. In contrast, gelatin, which is a fibrous protein, had only a slight dependency on pH.<br>(2) The foam formation of proteins varied with the differences of cations in the solution. The enhanced potential were Na<sup>+</sup>=K<sup>+</sup>>Mg<sup>2+</sup>>Sr<sup>2+</sup>>>Ca<sup>2+</sup> in order. Anions had no effect on foaming capacity.<br>(3) The foaming capacity of casein was elevated with the addition of LAS (sodium linear-dosecylbenzenesulfonate). In contrast, the capacity of casein was lowered by the presence of SDS (sodium dodecyl suophate).<br>(4) Globular proteins showed denaturation, dissociation and association with pH and coexistent substances, and these change affected foam formation.<br>It is possible to generate the optimum amount of proteinaceous foam by taking advantage of each protein's unique characteristics.
DOI： 10.2965/jswe.23.108
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A Test Method for Measuring the Foaming Capacity of Protein Solutions Reviewed
SUZUKI Yoshihiro, MARUYAMA Toshiroh
Japan journal of water pollution research 23 ( 2 ) 122 - 125 2000.2
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japan Society on Water Environment
In order to use protein as a chemical to bring about effective foam separation, it is necessary to understand foaming capacity and the factors that affect this capacity. In this study, the effect of various protein solutions on foaming capacity were measured using a bubbling column. The test compounds were casein, soy protein, albumin, hemoglobin, gelatin, and LAS.<br>The foaming capacity of a protein and a surfactant were evaluated by determining the height of the foam at the time of splitting or decaying within the bubbling column under constant conditions of air flow, volume of test solution, and water temperature. The most effective protein was casein followed by hemoglobin, soy protein, gelatin, and albumin. The capacity of casein was comparable to that of LAS. Under appropriate conditions, 0.3<i>l</i>-air · min<sup>-1</sup>, 100m<i>l</i> sample, 20°C, and pH6.9, the concentrations of casein and LAS required to make a foam height of 30cm (column diameter, 2.6cm) were 4.4mg · <i>l</i><sup>-1</sup> and 4.6mg · <i>l</i><sup>-1</sup>, respectively.
DOI： 10.2965/jswe.23.122
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SUZUKI Yoshihiro, MARUYAMA Toshiroh, SATO Daisuke, KANDA Takeshi, MICHISHITA Tamotsu, Yoshihiro Suzuki, Toshiroh Maruyama, Daisuke Sato, Takeshi Kanda, Tamotsu Michishita, Department of Civil and Environmental Engineering Miyazaki University, Department of Civil and Environmental Engineering Miyazaki University, Department of Civil and Environmental Engineering Miyazaki University, Fisheries Research Laboratory Miyazaki University, Fisheries Research Laboratory Miyazaki University
NSUGAF 66 ( 1 ) 1 - 9 2000.1
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：The Japanese Society of Fisheries Science
In this study, water quality and mass balances in the closed recirculating culture of Japanese flounder were examined. The closed system consisted of rearing thank, foam-separation and nitrification units, pH and temperature controlling units, and a circulation pump. Fish were fed commercial pellet diet for 90 days. Average values of oxygen saturation and turbidity in the rearing water through the culture were 96% and 3.1 TU, respectively. Ammonia was mostly kept at less than 1mg-N/l, and nitrate increased linearly according to the amount of diet supplied. It is estimated that 33% of dietary carbon (C) was accumulated in the fish, as well as 52% of nitrogen (N), and 26% of phosphorus (P). The carbon released as CO<SUB>2</SUB> was 63%. Nitrate in the rearing water corresponded to 28% of N-input, and 66% of P was settled in the nitrification unit as precipitate.
DOI： 10.2331/suisan.66.1
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Suzuki Y., Maruyama T., Sato D., Kanda T., Michishita T.
Nippon Suisan Gakkaishi (Japanese Edition) 66 ( 1 ) 1 - 9 2000.1
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Nippon Suisan Gakkaishi (Japanese Edition)
In this study, water quality and mass balances in the closed recirculating culture of Japanese flounder were examined. The closed system consisted of rearing thank, foam-separation and nitrification units, pH and temperature controlling units, and a circulation pump. Fish were fed commercial pellet diet for 90 days. Average values of oxygen saturation and turbidity in the rearing water through the culture were 96% and 3.1 TU, respectively. Ammonia was mostly kept at less than 1 mg-N/l, and nitrate increased linearly according to the amount of diet supplied. It is estimated that 33% of dietary carbon (C) was accumulated in the fish, as well as 52% of nitrogen (N), and 26% of phosphorus (P). The carbon released as CO 2 was 63%. Nitrate in the rearing water corresponded to 28% of N-input, and 66% of P was settled in the nitrification unit as precipitate.
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SUZUKI Yoshihiro, MARUYAMA Toshiroh, TAKEMOTO Susumu, ODA Risa
Journal of Japan Society on Water Environment 22 ( 11 ) 896 - 903 1999.11
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japan Society on Water Environment
If a closed recirculating aquaculture system that need not discharge the effluents is developed, a large amount of the pollutant load on water bodies can be reduced. In this study, rearing experiment of eel were reared in a closed recirculating system. The system consisted of a fish tank, a foam separation unit, nitrification and denitrification units, pH and temperature control units, and a circulation pump. The foam separation unit has an air inhalation-type aerator and supplies numerous bubbles into the rearing water. Fish were fed a commercial diet for 3 months.The survival rate of eel was 100% during the experiment under the congested condition. The foam separation unit kept oxygen saturation in the rearing water at about 90%. Furthermore, colloidal matters were absorbed on stable foam formed from eel mucus and were removed from the rearing water by foam separation. Ammonia oxidation and the removal of suspend matters were accomplished rapidly at the same time with the nitrification unit, and ammonia concentration and turbidity were kept at less than 0.21mg-N·l-1 and 0.981 TU, respectively. When the denitrification process was operated, nitrate accumulated in the rearing water was reduced to a concentration less than 7mg-N·l-1. Based on these results, an intensive aquaculture of freshwater fish such as eel can be achieved by a closed recirculating system.
DOI： 10.2965/jswe.22.896
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MARUYAMA Toshiroh, SUZUKI Yoshihiro, SATO Daisuke, KANDA Takeshi, MICHISHITA Tamotsu, Toshiroh Maruyama, Yoshihiro Suzuki, Daisuke Sato, Takeshi Kanda, Tamotsu Michishita, Department of Civil and Environmental Engineering Miyazaki University, Department of Civil and Environmental Engineering Miyazaki University, Department of Civil and Environmental Engineering Miyazaki University, Fisheries Research Laboratory Miyazaki University, Fisheries Research Laboratory Miyazaki University
NSUGAF 65 ( 5 ) 818 - 825 1999.9
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：The Japanese Society of Fisheries Science
Rearing experiment of Japanese flounder Paralichthys olivaceus was conducted with two different culture systems, a closed recirculating system having a foam-separation unit and a flow-through system in which natural seawater was continuously supplied. The closed recirculating system consisted of a fish tank of 1m<SUP>3</SUP> in water volume, foam-separation and nitrification units, pH and temperature controlling units, and a circulation pump. A key feature of this system is the foam-separation unit, which is equipped with an air inhalation-type aerator that vigorously injects numerous fine bubbles into the rearing water. Fish were fed commercial pellet diet for 90 days. The survival rate of fish was 93.5%, and the gross weight of fish increased from 28.4kg to 50.0kg during the experiment in the closed recirculating system without the effluent. In the flow-through system, the survival rate was 98%, and the weight increased from 33.8kg to 64.1kg. Based on these results, intensive aquaculture can be achieved with the closed recirculating system.
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The role of terrestrial humic substances on the shift of kelp community to crustose coralline algae community of the southern Hokkaido Island in the Japan Sea Reviewed
K. Matsunaga, T. Kawaguchi, Y. Suzuki, G. Nigi
Journal of Experimental Marine Biology and Ecology 241 ( 2 ) 193 - 205 1999.8
Language：English Publishing type：Research paper (scientific journal)
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TAKAMI Tohru, MARUYAMA Toshiroh, SUZUKI Yoshihiro, MIURA Akio
Journal of Japan Society on Water Environment 22 ( 1 ) 29 - 34 1999.1
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japan Society on Water Environment
In the short-term bioassay using Porphyra yezoensis conchospores, it is basically important to clarify the most appropriate exposure period and end point to evaluate the effects of toxicants on the growth of Porphyra yezoensis conchospores. In this study, short-term bioassays (120 hours) were conducted on copper (Cu) and monochloramine (NH2Cl), which have different chemical characteristics. Three kinds of end points (survival ratio, germination ratio and growth ratio) at exposure periods every 24 hours were compared to define the most appropriate exposure period and end point.The minimum LOECs (lowest-observed-effect concentration) for Cu occurred at exposure period of 96 hours in the three kinds of end points. The 96-h LOECs from the survival ratio, the germination ratio and the growth ratio were 0.046 ± 0.026mg Cu·l-1 (n=5), 0.033 ± 0.027mg Cu·l-1 (n=5), 0.021 ± 0.019mg Cu·l-1 (n=5), respectively. The minimum LOECs for NH2Cl occurred at exposure period of 96 hours in the survival ratio and the growth ratio of 0.093 ± 0.026mg Cl2·l-1 (n=5) and 0.038 ± 0.016mg Cl2·l-1 (n=5), respectively. The minimum LOEC (0.036 ± 0.000mg Cl2·l-1, n=5) for NH2Cl from the germination ratio occurred at the exposure period of 48 hours.Therefore, taking into account of the simplicity of bioassay and its sensitivity to toxicants, it is concluded that the most appropriate exposure period and end point is 96 hours for Cu and 48 hours for NH2Cl judging from the end point of the germination ratio.
DOI： 10.2965/jswe.22.29
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海藻（スサビノリ殻胞子）を用いた生物検定における適切な暴露時間と判定指標の検討 Reviewed
高見徹，丸山俊朗，鈴木祥広，三浦昭雄
水環境学会誌, 22巻, 1号, 29-34頁 22 ( 1 ) 29 - 34 1999.1
Language：Japanese Publishing type：Research paper (scientific journal)
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Maruyama T., Suzuki Y., Sato D., Kanda T., Michishita T.
Nippon Suisan Gakkaishi (Japanese Edition) 65 ( 5 ) 818 - 825 1999.1
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Nippon Suisan Gakkaishi (Japanese Edition)
Rearing experiment of Japanese flounder Paralichthys olivaceus was conducted with two different culture systems, a closed recirculating system having a foam-separation unit and a flow-through system in which natural seawater was continuously supplied. The closed recirculating system consisted of a fish tank of 1 m 3 in water volume, foam-separation and nitrification units, pH and temperature controlling units, and a circulation pump. A key feature of this system is the foam-separation unit, which is equipped with an air inhalation-type aerator that vigorously injects numerous fine bubbles into the rearing water. Fish were fed commercial pellet diet for 90 days. The survival rate of fish was 93.5%, and the gross weight of fish increased from 28.4 kg to 50.0 kg during the experiment in the closed recirculating system without the effluent. In the flow-through system, the survival rate was 98%, and the weight increased from 33.8 kg to 64.1 kg. Based on these results, intensive aquaculture can be achieved with the closed recirculating system.
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MARUYAMA Toshiroh, SUZUKI Yoshihiro
Doboku Gakkai Ronbunshuu B 15 i - xiv 1999
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japan Society of Civil Engineers
DOI： 10.2208/prooe.15.i
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TAKAMI Tohru, MARUYAMA Toshiroh, SUZUKI Yoshihiro, KAIGA Nobuyoshi, MIURA Akio
Journal of Japan Society on Water Environment 21 ( 11 ) 711 - 718 1998.11
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japan Society on Water Environment
Chlorination of secondary effluents produce strong toxic substance such as monochloramine or oxidant on growth of aquatic organisms. Therefore, alternative disinfection methods to chlorination should be considered. However, the effect of effluents disinfected with the alternatives on seaweed has not been clarified. In this study, the toxicity of secondary effluents disinfected with chlorine (NaOCl), chlorine dioxide (ClO2), ozone (O3), and ultraviolet rays (UV) were investigated by bioassay using Porphyra yezoensis conchospores. Secondary effluents used in this study were disinfected with disinfectants or rays which inactivated coliform groups in secondary effluents of 99.9%. Lowest-observed-effect concentrations (LOECs) of secondary effluents disinfected with NaOCl, ClO2, and O3 for germination ratio of conchospores were 1.0, 10, and 100% of effluent concentration, respectively. The toxicity of effluents disinfected with NaOCl and ClO2 were very strong. In contrast, the effluents disinfected with UV rays did not affect the germination of conchospores. The toxicity of effluents disinfected with NaOCl, ClO2, and O3 were considered to depend on initial total residual oxidant (TRO). Dechlorination with Na2SO3 of effluents disinfected with NaOCl or ClO2 did not affect the germination of conchospores, even in 100% of effluent concentration.
DOI： 10.2965/jswe.21.711
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藻類増殖阻害試験における生物量の測定方法が毒性評価に及ぼす影響 Reviewed
鈴木祥広，森下玲子，高見徹，丸山俊朗
環境工学研究論文集 35 101 - 109 1998.11
Language：Japanese Publishing type：Research paper (scientific journal)
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Riverine input of bioavailable iron supporting phytoplankton growth in Kesennuma Bay Reviewed
K. Matsunaga, J. Nishioka, K. Kuma, T. Toya, Y. Suzuki
Water Research 32 ( 11 ) 3436 - 3442 1998.11
Language：English Publishing type：Research paper (scientific journal)
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Riverine input of bioavailable iron supporting phytoplankton growth in Kesennuma Bay (Japan)
Matsunaga K., Nishioka J., Kuma K., Toya K., Suzuki Y.
Water Research 32 ( 11 ) 3436 - 3442 1998.11
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Water Research
The effects of riverain iron and nutrient inputs on phytoplankton growth in Kesennuma Bay were studied. The effects of iron and Fulvic acid-iron complex additions on phytoplankton growth were studied in iron-enriched and -limited culture experiments of coastal marine diatom Chaetoceros sp. (the dominant species inside and outside of the bay) using media prepared from bay and outer waters. Bay water is not iron-limited. The addition of Fe(lII) to bay water or autoclaved bay water gave no increase in cell yield. However, when bay water was autoclaved after UV-irradiation, there was little growth. This suggests that the UV irradiation destroyed organic compounds that affected iron bioavailability. Outer water is iron-limited. The addition of Fe(III) to outer water increased cell yield and iron-enriched outer water prepared by autoclaving after adding Fulvic acid-Fe increased also cell yield. When outer water after adding Fe(III) was autoclaved, there was little growth. This suggests that fulvic acid made the iron bioavailable. The riverain inputs of organically bound iron, such as fulvic acid-Fe, and nutrients probably play an important role for supporting phytoplankton growth in the bay.
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SUZUKI Yoshihiro, MARUYAMA Toshiroh, TAKAMI Tohru, MIURA Akio
Journal of Japan Society on Water Environment 21 ( 10 ) 670 - 675 1998.10
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japan Society on Water Environment
Effects of suspended and accumulated kaolinite particles on the adhesion and the development of Undaria pinnatifida zoospores on the substratum were investigated under the following three types of condition; Case-1: suspended particles together with zoospores, Case-2: zoospores released after accumulation of particles on the substratum, and Case-3 : accumulated particles on zoospores adhered on the substratum.Case-2 was the most effective inhibition for the adhesion of zoospores, and 50% of effective concentration of particles (EC50) was 29 μg·cm-2. It was difficult for gametophytes to develop to sporophytes in Case-3. Furthermore, in comparing Undaria zoospores with Porphyra conchospores, the zoospores showed higher tolerance to suspended particles.
DOI： 10.2965/jswe.21.670
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森林起源のフルボ酸鉄がコンブやワカメの生長に果たす効果 Reviewed
松永 勝彦, 和 吾郎, 鈴木 祥広, 安井 肇, ディーン グリサダ
日本海水学会誌 52 ( 5 ) 315 - 318 1998.10
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：日本海水学会
森林起源のフルポ酸鉄が海藻に果たす効果について, コンブと.ワカメの遊走子 (胞子) ならびにコンブの幼体を用いて検討した.遊走子が卵を形成する割合を フルポ-Fe, EDTA-Fe, 無定形水酸化鉄をそれぞれ0.2μMとして, 10℃で培養した.30日後, 卵形成はそれぞれ70, 54, 25%であった. ワカメの卵形成についてはそれぞれ55, 42, 25%であった.また, 幼体の生長はフルポ酸-Fe共存下では無定形水酸化鉄に比べ3倍も速かった.<BR>コンブによる鉄の摂取速度を フルポ酸-Feあるいは無定形水酸化鉄を用いて測定したが, フルポ酸-Feの方が1オーダ速い摂取速度を示した. フルポ酸-Fe共存下で卵形成割合が高いことならびに幼体の生長が速いことは鉄摂取速度の相違によって説明出来ると思われる.<BR>なお, 本研究はソルト・サイエンス研究財団のプロジェクト研究「 沿岸海水環境の変化と生態系への影響」の一部として行なった.プ ロジェクトリーダーである東京大学名誉教授堀部純男先生にまた有益なご助言をいただいた東京農業大学名誉教授杉二郎先生に心から謝意を表します.