Papers - SUZUKI Yoshihiro
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Genotypic Characterization of Enterococci Collected from a Coastal Area Using PFGE Reviewed
FURUKAWA Takashi, YOSHIDA Terutoyo, SUZUKI Yoshihiro
J. Env. cons. eng 40 ( 3 ) 138 - 143 2011.3
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Society of Environmental Conservation Engineering
Development of microbial source tracking (MST) is crucial to ensure public health and bacteriological safety in coastal areas. In this study, pulsed-field gel electrophoresis (PFGE) , utilized for pollution source tracking of hospital infections, was applied to the aquatic environment as a MST method. <I>Enterococcus faecium</I> and <I>Entecococcus faecalis</I>, fecal indicator bacterias, were isolated and identified from a port area, and the genotype was analyzed by PFGE. A total of 230 enterococcal strains were isolated by PFGE, and forty strains were identified as <I>E. faecium</I> using both PCR and the Api 20 Strep test. Dendrogram analysis of the PFGE types revealed that all the 40 <I>E. faecium</I> strains can be classified separately into 7 different groups at a 0.7 similarity level. The genotypic characterization of <I>E. faecium</I> from the coastal port area revealed diverse types. It is suggested that PFGE can be used to differentiate and characterize fecal pollution indicating bacteria by applying it to the aquatic environment, as well as provide detailed information to specify the pollution source.
DOI： 10.5956/jriet.40.138
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マイクロプレートを用いたバイオアッセイによる宮崎県五ヶ瀬川水系の藻類増殖制限物質の検索 Reviewed
鈴木祥広, 黒澤津翔, 金丸祐加, 高見徹, 鬼束幸樹
用水と廃水 53 ( 2 ) 134 - 141 2011.2
Language：Japanese Publishing type：Research paper (scientific journal)
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Fall J., Chakraborty G., Kono T., Maeda M., Suzuki Y., Itami T., Sakai M.
Fisheries Science 77 ( 1 ) 129 - 134 2011
Language：English Publishing type：Research paper (scientific journal) Publisher：Fisheries Science
Vibrio nigripulchritudo is considered one of the major pathogens threatening shrimp aquaculture. In this study, we developed a novel and highly specific quantitative loop-mediated isothermal amplification (Q-LAMP) assay. A set of four specific primers were designed targeting the V. nigripulchritudo intergenic spacer region. The reaction time and temperature were optimized for 60 min at 63°C. Quantitative analysis was then performed by measuring the turbidity of the reaction solution using a real-time turbidimeter, allowing for quantification of the initial DNA concentration with a sensitivity of 10 2 copy numbers equivalent to 2.3 colony forming units/ml or 0.3 fg/μl. The LAMP assay was able to specifically detect two representative strains of V. nigripulchritudo, whereas other Vibrio and non-Vibrio species were not amplified. A standard curve was generated for V. nigripulchritudo by plotting the threshold time (T t ) versus the log of bacterial number. A high correlation coefficient (R 2 = 0.9749) was observed for the Q-LAMP reaction. In conclusion, Q-LAMP assay is a sensitive, rapid, and simple tool that can be used for the detection and quantification of V. nigripulchritudo in shrimp, thereby facilitating surveillance of vibriosis infection. © 2010 The Japanese Society of Fisheries Science.
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Study on characteristics of early phase leachate from simulated carcasses burial site
sekito tomoo, dote yutaka, morita tetsuo, inagaki hitone, suzuki yoshihiro
Proceedings of the Annual Conference of Japan Society of Material Cycles and Waste Management 22 ( 0 ) 199 - 199 2011
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japan Society of Material Cycles and Waste Management
Recently mass carcasses burial has been occurred in order to prevent further spread of the animal disease such as foot and mouth disease and bird flu. It is important to estimate environmental contaminations from the burial sites. In this study, leachate characteristics from carcasses burial site were investigated by laboratory scale column test. In this study, rats were used as modeled carcasses. From the analysis of the leachate, significant high concentrations of COD (20,000-40,000 mg/L) and NH3-N (5,000-10,000 mg/L) in the early period of experiment were determined. The generation of initial leachate from a column with saw dust as a part of soil could be delayed. Further study for kinetics of organic degradation and material balance is necessary.
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バッチプラントでの硫酸を用いた鶏ふん焼却灰からのリン回収に関する研究 Reviewed
土手裕, 関戸知雄, 太田靖子, 鈴木祥広
環境工学研究論文集 47 451 - 458 2010.11
Language：Japanese Publishing type：Research paper (scientific journal)
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物理・化学的性状解析によるダム貯水池の長期濁水化の原因となる難沈降性粒子の発生源追跡手法の提案 Reviewed
村上俊樹, 鈴木祥広, 瀬崎満弘, 伊藤健一, 中尾登志雄
環境工学研究論文集 47 199 - 206 2010.11
Language：Japanese Publishing type：Research paper (scientific journal)
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沿岸レクリエーションエリアにおけるふん便性細菌汚染の調査 Reviewed
古川 隼士, 川畑 勇人, 鈴木 祥広
環境技術 39 ( 8 ) 493 - 499 2010.8
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Society of Environmental Conservation Engineering
本研究では，宮崎県内の沿岸域における細菌学的調査を実施した．沿岸水試料のふん便性大腸菌群（FC）とふん便性連鎖球菌（FS）は，それぞれ０～1.9×10<sup>3</sup>CFU/100mLと０～2.0×10<sup>3</sup>CFU/100mLで検出され，浜砂試料では，それぞれ０～3.0×10<sup>3</sup>CFU/100g-dryと０～1.4×10<sup>4</sup>CFU/100g-dryで検出された．海水浴場やサーフィンエリアのレクリエーションエリアでは，降雨による影響が考えられる６月と10月の調査日を除けば，晴天時では両細菌ともに100CFU/100mL以下であった．各細菌数は，季節ごと，地点ごとにおいて変動が大きかった．
DOI： 10.5956/jriet.39.493
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Spawning of Kuruma Shrimp in a Closed Recirculating Aquaculture System after Eyestalk Ablation Reviewed
SUZUKI Yoshihiro, SAGISU Yuji, SUZUKI Takahiko, MEKATA Thoru, KONO Tomoya, KOSHIO Shunsuke, SAKAI Masahiro, ITAMI Toshiaki
58 ( 2 ) 161 - 166 2010.6
Language：Japanese Publishing type：Research paper (scientific journal)
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Behavior of Fecal Bacteria in a Coastal Urban River
FURUKAWA Takashi, TANAKA Akihiko, YOSHIDA Terutovo, SUZUKI Yoshihiro
J. Env. cons. eng 39 ( 3 ) 170 - 176 2010.3
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Society of Environmental Conservation Engineering
DOI： 10.5956/jriet.39.170
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Biota of fecal bacteria concentrated in stable foam formed along water's edge in coastal zones Reviewed
Furukawa T., Yoshida T., Suzuki Y.
Bubble Science, Engineering and Technology 2 ( 1 ) 25 - 30 2010.3
Language：English Publishing type：Research paper (scientific journal) Publisher：Bubble Science, Engineering and Technology
Since the coastal zone plays an important role in recreation, humans might be directly or indirectly exposed to pathogenic bacteria. Along the water's edge at the coast, stable yellowish brown foam generated by breaking waves is observed frequently. We examined the foam, and determined the fecal bacteria count in coastal water, sand and the foam on the Miyazaki coast, Japan. Fecal coliform and fecal streptococcus counts in the foam were markedly higher than those in the coastal water and sand. Furthermore, the concentration of dissolved organic carbon in the foam was much higher than that in the coastal water. We concluded that the fecal bacteria were concentrated in the foam formed by bubbles generated in the water and that the dissolved organic substances exhibit surface activity. In addition, the main species of fecal streptococcus in the foam was enterococcus faecium, which is often used as an indicator of fecal pollution. © 2010 W. S. Maney & Son Ltd.
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河川水と海水のバッチ式混合実験におけるエストロゲンの挙動（共著）
古川隼士, 鈴木祥広
宮崎大学工学部紀要 2010.3
Language：Japanese Publishing type：Research paper (bulletin of university, research institution)
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海水との混合による都市河川水中のふん便性細菌の挙動に関する基礎的検討.共著） Reviewed
古川隼士, 田中昭彦, 吉田照豊, 鈴木祥広
環境技術 39 ( 3 ) 170 - 176 2010.3
Language：Japanese Publishing type：Research paper (scientific journal)
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Genotypic analysis of enterococci collected from a coastal sone by pulsed-field gel electrophoresis (PFGE) for application of microbial source tracking."jointly worked" Reviewed
Furukawa, T., Yoshida, T. and Suzuki, Y.
ASPIRE2009 65 - 65 2009.10
Language：English Publishing type：Research paper (international conference proceedings)
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A simple non-enzymatic method for the preparation of white spot syndrome virus (WSSV) DNA from the haemolymph of Marsupenaeus japonicus using FTA matrix cards."jointly worked" Reviewed
Sudhakaran, R., Mekata, T., Kono, T., Supamattaya, K., Linh, N.T.H., Suzuki, Y., Sakai, M. and Itami, T.
Journal of Fish Diseases 32 611 - 617 2009.10
Language：English Publishing type：Research paper (scientific journal)
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Residual of 17β-estradiol in digestion liquid generated from a biogas plant using livestock waste
Suzuki Y., Kubota A., Furukawa T., Sugamoto K., Asano Y., Takahashi H., Sekito T., Dote Y., Sugimoto Y.
Journal of Hazardous Materials 165 ( 1-3 ) 677 - 682 2009.6
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Hazardous Materials
A biogas plant using livestock waste in which a methane fermentation process is applied is a useful facility for generating energy. The digestion liquid generated from the biogas plant as a residue has high potential for use as a crop fertilizer. However, high-density estrogens such as 17β-estradiol (E2) are included in livestock waste, and there is little information on the behavior of E2 in the digestion liquid. In this study, a survey of E2 concentration at each process in a biogas plant using livestock waste was carried out. In addition, the efficiencies of E2 removal from the digestion liquid by activated carbon adsorption and soil infiltration were examined. The total concentration of E2 in raw livestock waste was reduced to 2 μg/l after treatment, and the removal efficiency of E2 was about 80% for the plant. The methane fermentation process is important not only for the generation of methane but also for the removal of E2. The proportion of E2 conjugates comprising the total E2 concentration was 10% or less in all treated samples. In the plant, there is no likelihood of an increase in estrogen activity by the cleaving of E2 conjugates. By carrying out activated carbon adsorption to remove E2 from the digestion liquid, a large portion of E2 was removed from the digestion liquid, but an E2 concentration of 0.5 μg/l still remained in the treated digestion liquid. In contrast, it was possible to purify the digestion liquid to an E2 concentration of less than 0.002-0.011 μg/l by soil infiltration. It is thus possible to utilize the digestion liquid as a fertilizer without causing aquatic environmental pollution, but factors such as application rate, soil characteristics, and the E2 concentration of digestion liquid should be considered first. © 2008 Elsevier B.V. All rights reserved.
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宮崎県小丸川水系のダム堆積土砂に含まれる難沈降粒子の物理・化学的性状 Reviewed
鈴木祥広, 出口近士, 関戸知雄, 塩盛弘一郎, 杉尾哲
土木学会論文集 65 ( 1 ) 16 - 25 2009.2
Language：Japanese Publishing type：Research paper (scientific journal)
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Suzuki Y., Narimatsu S., Furukawa T., Mekata T., Kono T., Sakai M., Itami T., Katayama H.
Separation Science and Technology 44 ( 3 ) 569 - 584 2009.2
Language：English Publishing type：Research paper (scientific journal) Publisher：Separation Science and Technology
To prevent the spread of acute gastroenteritis, which prevails during winter and is called the winter vomiting disease, it is necessary to develop a technology for the detection and removal of noroviruses (NVs) from contaminated water. In this study, the removal from municipal wastewater and concentration in foam of NVs using dispersed bubbles and milk casein as a surface-active protein were examined. Real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) as a new nucleic acid amplification method was used for NV detection. Although NV-GII was detected in two wastewater samples, NV-GI was not detected. By foam separation using dispersed bubbles, NV-GII was removed from wastewater and concentrated into the generated foam. The concentration of NV-GII in the collapsed foam water was more than approximately 8-fold higher than that in the wastewater. Because the generated foam can be analyzed by real-time RT-LAMP, it is possible to sensitively monitor NV pollution in aqueous environments. Here, we show for the first time the removal from aqueous solution and concentration of NVs in foam using dispersed bubbles and a surface-active substance.
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Mekata T., Sudhakaran R., Kono T., U-taynapun K., Supamattaya K., Suzuki Y., Sakai M., Itami T.
Journal of Virological Methods 162 ( 1-2 ) 81 - 87 2009.1
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Virological Methods
A real-time reverse transcription loop-mediated isothermal amplification (real-time RT-LAMP) method was applied for detecting the replicase polyprotein-encoding gene of yellow head virus (YHV) in shrimp, Penaeus monodon. It is a novel, gene-specific assay that amplifies nucleic acid with high specificity, sensitivity and rapidity under isothermal conditions using a set of six specially designed primers that recognize eight distinct sequences of the target gene. This method works with even low copies of DNA and is based on magnesium pyrophosphate turbidity detection by an inexpensive photometer for quantitative analysis. A user-friendly protocol was developed with optimal conditions standardized at 63 °C for 60 min. With this protocol, the assay sensitivity was 10 times higher than the widely used YHV nested RT-PCR system. Cross-reactivity analysis using other shrimp virus DNA/cDNA and YHV-negative shrimp demonstrated high specificity of the assay. The real-time RT-LAMP method was performed also for an internal control gene, EF-1α, to compare with the expressions of the YHV gene in different organs of infected shrimp, and the resulting standard curves showed high correlation coefficient values. These results suggest that this assay is applicable widely as a new quantitative detection method in the pursuit of YHV-free shrimp culture. © 2009 Elsevier B.V. All rights reserved.
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SUZUKI Yoshihiro, DEGUCHI Chikashi, SEKITO Tomoo, SHIOMORI Koichiro, SUGIO Satoru
Journal of Japan Society of Civil Engineers, Ser. G (Environmental Research) 65 ( 1 ) 16 - 25 2009
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japan Society of Civil Engineers
The characteristics of fine particles such as grain size and mineral composition contained into the sediment in three dam reservoirs (Dokawa, Matsuo and Kawaharu dams) along river Omaru in Miyazaki were investigated. The size of particles, which were difficult to settle as the cause factor of the turbid dam water for a long period, were average 2μm, and the shape was plane. By examining from the particles composition, the origin of fine particles in Matsuo and Kawaharu dams was identical with the fine particles in sediment of Dokawa dam. The content of fine particles was 10 mg/g-dry sediment and the settling velocity was markedly low (2 mm/hr). It was indicated that the clarification of suspension water in the dam reseroir containing the large amount of fine particles would be difficult by gravity sedimentation.
DOI： 10.2208/jscejg.65.16
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SUZUKI Yoshihiro, SUZUKI Takahiko, SAGISHU Yuji, MEKATA Thoru, KONO Tomoya, KOSHIO Shunsuke, YOKOYAMA Saichiro, SAKAI Masahiro, ITAMI Toshiaki
56 ( 4 ) 479 - 485 2008.12
Language：Japanese Publishing type：Research paper (scientific journal)