Papers - SUZUKI Yoshihiro
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Tracing the source of difficult to settle fine particles which cause turbidity in the Hitotsuse reservoir, Japan Reviewed
Murakami T, Suzuki Y, Oishi H, Ito K, Nakao T
Journal of Environmental Management 120 37 - 47 2013.3
Language：English Publishing type：Research paper (scientific journal)
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FURUKAWA TAKASHI, SUZUKI YOSHIHIRO
Microbes and Environments 28 ( 4 ) 444 - 449 2013
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japanese Society of Microbial Ecology · The Japanese Society of Soil Microbiology
This study aimed to identify specific river sources of fecal contamination by applying pulsed-field gel electrophoresis (PFGE) to environmental water samples from a recreational beach in Japan. The genotypes of all <i>Enterococcus faecium</i> and <i>Enterococcus faecalis</i> strains used as indicators of fecal pollution on the recreational beach and rivers were analyzed by PFGE, and the PFGE profiles of the strains were classified at a 0.9 similarity level using dendrogram analysis. PFGE types of <i>E. faecium</i> isolated from Sakai River or urban drainage were classified in the same cluster. Therefore, the probable sources of fecal pollution on the recreational beach were Sakai River and urban drainage. The approaches for microbial source tracking employed in this study used PFGE with <i>Enterococcus</i> species as an indicator can be a potential tool to specify the source(s) of fecal pollution and contribute to improved public health in coastal environments.<br>
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Research on gasification rate of organic matter in modeled burial livestock and odor generation
Proceedings of the Annual Conference of Japan Society of Material Cycles and Waste Management 24 ( 0 ) 2013
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japan Society of Material Cycles and Waste Management
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Suzuki Yoshihiro, Takeshita Shinichi, Sekito Tomoo, Inagaki Hitone
Japanese Journal of Grassland Science 58 ( 4 ) 269 - 276 2013
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japanese Society of Grassland Science
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FURUKAWA Takashi, SHIMAUCHI Hidetaka, SUZUKI Yoshihiro
J. Env. cons. eng 42 ( 10 ) 617 - 624 2013
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Society of Environmental Conservation Engineering
By applying pulsed-field gel electrophoresis(PFGE)to the aquatic environment as a source tracking method of fecal pollution, the temporal variability of genetic patterns of<i> Enterococcus faecium</i>, as fecal indicator bacterium, was examined. Surface water samples were collected from a fixed point of the Miyazaki municipal river during a two-month period. <i>E. faecium</i> strains were identified and collected from each water sample.Genotypes(PFGE types)of all <i>E. faecium</i> strains were analyzed by PFGE. Then, the similarities between the PFGE types obtained from the first day and those from the other days were compared using dendrogram analysis. The PFGE type of <i>E. faecium</i> was not significantly changed for the initial three days. After the seventh day, the dominant PFGE types were changed. The PFGE types of the 30th and 60th days were not very similar to those of the first day. It was indicated that genetic patterns of <i>E. faecium</i> might change to completely dissimilar patterns in a fixed point of the municipal river. Therefore, it is very important to consider the temporal variability of genetic pattern of fecal indicator bacteria for source tracking of fecal pollution.
DOI： 10.5956/jriet.42.617
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一ツ瀬ダム上流域における長期濁水化に 関与する土砂分布地点と地質構造との関係 Reviewed
村上俊樹, 鈴木祥広, 大石博之, 中尾登志雄
土木学会論文集G（環境） 68 ( 7 ) 251 - 257 2012.12
Language：Japanese Publishing type：Research paper (scientific journal)
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豚ふん尿の熱処理によるメタン発酵能向上に関する研究 Reviewed
土手裕, 関戸知雄, 後藤吉史, 鈴木祥広
土木学会論文集G（環境） 68 ( 7 ) 433 - 451 2012.12
Language：Japanese Publishing type：Research paper (scientific journal)
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Suzuki Y., Kanda N., Furukawa T.
Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering 47 ( 11 ) 1500 - 1505 2012.9
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
Enterococci such as Enterococcus faecalis and E. faecium are considered as the most suitable indicators of fecal pollution in an aquatic environment, and new methods for Enterococcus determination have been developed, namely, membrane filtration (MF) using membrane-Enterococcus indoxyl-β-D-glucoside agar (mEI) and defined substrate technology (DST) using Enterolert®. This study used PCR analysis to identify E. faecalis and E. faecium among Enterococcus strains in river water isolated using both mEI plates and Enterolert® trays. There was a significantly high correlation between MF and DST in terms of enterococcal counts for river water samples. The combined percentages of E. faecalis and E. faecium with respect to the total number of all strains obtained using mEI plates and Enterolert® trays were approximately 30% and > 30%, respectively. Other than E. faecalis and E. faecium, a large number of Enterococcus species were unspecified in the actual urban river samples. A comparison of the predominance of E. faecalis and E. faecium found that the abundance of a species depended on the sampling river and date. E. faecium is a non-predominant species in intestinal and fecal Enterococci, and it was one of the main Enterococcus species detected in surface water. Copyright © Taylor & Francis Group, LLC.
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置砂施工に用いるダム堆砂の洗浄工程で発生する濁水の簡易凝集処理 Reviewed
鈴木祥広
用水と廃水 54 ( 8 ) 605 - 612 2012.8
Language：Japanese Publishing type：Research paper (scientific journal)
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Monitoring of Fecal Bacteria in a Coastal Recreation Area during Summer Months Reviewed
FURUKAWA Takashi, KAWABATA Hayato, SUZUKI Yoshihiro
J. Env. cons. eng 41 ( 3 ) 138 - 145 2012.3
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Society of Environmental Conservation Engineering
Fecal coliform (FC)and enterococci(ENT), frequently used as fecal indicators were monitored in the coastal recreation area of Miyazaki, Japan, from June to September 2009. The FC and ENT counts in coastal water were found to range from Below detection limits(BDL)to 6.3× 10<sup>3</sup>CFU/100 mL and from BDL to 5.2× 10<sup>2</sup>CFU/100 mL, respectively. These counts varied depending on the numbers of sampling days and stations, and exceeded the criteria for marine recreation water recommended by the US Environmental Protection Agency during sampling events. Both bacteria showed a significant positive correlation with rainfall (FC:r=0.83, ENT:r=0.99), and a negative correlation with salinity (FC:r=-0.60, ENT :r=-0.75). In the identification test of enterococcal strains using PCR, <I>Enterococcus faecium</I> and <I>Enterococcus faecalis</I> were detected at high frequencies in some stations. Our findings demonstrate that during heavy rains, some stations in the coastal recreation area could be affected by fecal pollution from human and livestock feces with the inflow of inland water.
DOI： 10.5956/jriet.41.138
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ダム底泥に含まれる鉄が海藻ヤツマタモクの生長と増殖に及ぼす効果（共著） Reviewed
鈴木祥広, 濱崎祥大, 荒武久道, 齋藤剛
用水と廃水 54 ( 2 ) 136 - 144 2012.2
Language：Japanese Publishing type：Research paper (scientific journal)
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MURAKAMI Toshiki, SUZUKI Yoshihiro, OISHI Hiroyuki, NAKAO Toshio
PROCEEDINGS OF ENVIRONMENTAL AND SANITARY ENGINEERING RESEARCH 68 ( 7 ) III_251 - III_257 2012
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japan Society of Civil Engineers
Through a soil survey, the authors collected soil samples from different strata at locations where landslip had occurred in the upstream area of a dam reservoir suffering from long-term turbidity. And soil specimens that might be responsible for accelerating the turbidity were obtained from the soil samples, using a simple suspension method. The soil specimens were categorized into four risk levels, indicating their degree of turbidity. Further, we prepared a detailed geological block diagram, which specifies different geological structures based on metamorphic grades. As a result, it was found that there was a significant correlation between the distribution of geological structure and the locations where high-risk-level soil samples were collected. This information is useful for determination of the distribution of soil structure at specific locations where there is soil causing turbidity, contributing to effective countermeasures for long-term turbidity in dam reservoirs.
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Effect of thermal treatment of pig manure on methane fermentation
DOTE Yutaka, SEKITO Tomoo, GOTOH Yoshifumi, SUZUKI Yoshihiro
PROCEEDINGS OF ENVIRONMENTAL AND SANITARY ENGINEERING RESEARCH 68 ( 7 ) III_443 - III_451 2012
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japan Society of Civil Engineers
Thermal treatment of pig manure followed by batch and continuous methane fermentation was conducted in order to evaluate its effect on fermentation performance. Up to 200°C of thermal treatment, solubilization of VSS proceeded, but transformation of dissolved TOC with high molecule count into VFA was minimal. The high temperature led to retardation of initial gas generation and low gas generation rate. At 250°C, no gas generation occurred. At 150°C, methane generation per input VS with thermal treatment was 1.2 times greater than that without thermal treatment. The thermal treatment of standard pig manure was disadvantageous to net energy recovery. However, it was estimated that thermal treatment of manure with SS concentration of greater than 2.3 times the SS of standard manure increased net energy recovery over that without thermal treatment.
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Mass balance and gas generation from modeled column of livestock bulial site.
Fujiwara Naohiro, Sekito Tomoo, Dote Hiroshi, Suzuki Yoshihiro, Inagaki Hitone, Morita Tetsuo
Proceedings of the Annual Conference of Japan Society of Material Cycles and Waste Management 23 ( 0 ) 2012
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japan Society of Material Cycles and Waste Management
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Monitoring of Fecal Bacteria on Aoshima Beach, Miyazaki, Japan Reviewed
FURUKAWA Takashi, KAI Ryusuke, DOTE Yutaka, SUZUKI Yoshihiro
Japan journal of water pollution research 34 ( 12 ) 197 - 201 2011.12
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japan Society on Water Environment
Recently, coastal recreation areas have been polluted by pathogenic microorganisms from human and animal feces. In fact, it has been reported that the risk of infection by pathogens is not negligible in coastal areas. Fecal bacteria (e.g., fecal coliforms (FCs) and enterococci (ENTs)) were monitored on Aoshima Beach, Miyazaki, Japan, to understand the contamination by fecal bacteria, indicating the presence of pathogens in coastal recreation areas. Coastal water samples were collected at 5 stations on Aoshima Beach between May and August, 2010. The FC and ENT counts in coastal water were found to range from below detection limits (BDL) to 1.4×10<SUP>3</SUP> CFU·100 mL<SUP>-1</SUP> and from BDL to 4.6×10<SUP>2</SUP> CFU·100 mL<SUP>-1</SUP>, respectively. The monitoring carried out daily between June 23 and July 21 indicated that the concentrations of both fecal bacteria increased during rainfall, exceeding the guideline levels set by U. S. Environmental Protection Agency (i.e., FC, 800 CFU·100 mL<SUP>-1</SUP>; ENT, 104 CFU·100 mL<SUP>-1</SUP>). However, the high fecal bacterial counts decreased to approximately 10 CFU·100 mL<SUP>-1</SUP> within 3 days after the rainfall.
DOI： 10.2965/jswe.34.197
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鶏ふん焼却灰からのリン回収における硫酸除去のためのカルシウム源の評価に関する研究（共著） Reviewed
土手裕, 関戸知雄, 園田忠道, 矢野浩司, 鈴木祥広
土木学会論文集G(環境） 67 ( 7 ) 195 - 202 2011.11
Language：Japanese Publishing type：Research paper (scientific journal)
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Suzuki Yoshihiro, Narimatsu Shogo, Furukawa Takashi, IWAKIRI Akira, MIURA Miho, YAMAMOTO Shogo, KATAYAMA Hiroyuki
Journal of bioscience and bioengineering 112 ( 4 ) 369 - 372 2011.10
Language：English Publishing type：Research paper (scientific journal) Publisher：公益社団法人日本生物工学会
The monitoring of NVs in municipal wastewater by both real-time RT-LAMP and real-time RT-PCR, and the comparison of these two methods with respect to NV detection were carried out. The change in NVs detected by real-time RT-LAMP agreed well with that detected by real-time RT-PCR. In contrast, the correlation between the copy number determined by real-time RT-PCR and the threshold time (Tt) determined by real-time RT-LAMP obtained during monitoring was not significant (0.1<p) for both NV-GI and NV-GII.
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Furukawa T., Takahashi H., Yoshida T., Suzuki Y.
Microbes and Environments 26 ( 2 ) 181 - 183 2011.6
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Microbes and Environments
PFGE has potential applications in the source tracking of fecal pollution in aquatic environments. We tried to distinguish the genotypes of Enterococcus faecium collected from fecal-contaminated water using PFGE. Well identified 115 strains of E. faecium were classified into 25 PFGE patterns, and characteristics distinctive to each genotype were recognized. Analysis of the characteristics of genotypes using PFGE can be used to track source of fecal pollution.
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Application of PFGE to source tracking of fecal pollution in coastal recreation area: A case study in Aoshima Beach, Japan"jointly worked" Reviewed
Furukawa T, Yoshida T, Suzuki Y
Journal of Applied Microbiology 110 688 - 696 2011.5
Language：English Publishing type：Research paper (scientific journal)
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Suzuki Y., Suzuki T., Kono T., Mekata T., Sakai M., Itami T.
Journal of Virological Methods 173 ( 2 ) 227 - 232 2011.5
Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Virological Methods
The white spot disease virus (WSDV), which is most virulent in shrimp, is a cause of serious damage in the shrimp production industry. However, it is difficult to track the infection route and behaviour of WSDV in shrimp farms because it is present at extremely low concentrations in culture sea water. In this study, the concentration of WSDV in sea water foam was examined using dispersed bubbles and milk casein as a surface-active protein. WSDV concentrations were assessed using real-time PCR. When ferric colloid adsorption was performed prior to foam separation, WSDV was effectively removed from sea water and concentrated in the generated foam within 5. min. The removal efficiency was greater than 90% at the optimum iron and casein concentrations of 1. mg Fe/l and 1. mg/l, respectively. Furthermore, to analyse the dissolution of the collected ferric colloid, the WSDV concentration in the colloid-dissolved solution was set to be 200-fold higher than that found in raw water. This represents a novel method of concentrating WSDV for its detection in sea water using a combination of ferric colloid adsorption and foam separation that is easy to perform, rapid and efficient. © 2011 Elsevier B.V.