論文 - 丸山 治彦
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<i>Strongyloides stercoralis</i> colitis in a patient positive for human T-cell leukaemia virus with rheumatoid arthritis during an anti-rheumatic therapy: a case report. 査読あり 国際誌
Hashiba Y, Umekita K, Minami H, Kawano A, Nagayasu E, Maruyama H, Hidaka T, Okayama A
Modern rheumatology case reports 5 ( 1 ) 16 - 21 2021年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）
An elderly woman with rheumatoid arthritis (RA) presented with a chief complaint of abdominal pain and diarrhea while undergoing treatment with low-dose corticosteroids and abatacept. Endoscopic and histopathological findings revealed manifestations of ulcerative colitis (UC). An intermediate dose of corticosteroids and 5-aminosalicylic acid were administered. Abatacept was discontinued; the anti-TNF biologic, golimumab, was administered for treatment of both RA and UC. However, colitis worsened in response to this therapeutic regimen. Colonoscopy revealed severe mucosal lesions; larvae were detected in samples taken from multiple shallow mucosal ulcers. The patient was diagnosed with Strongyloides stercoralis colitis based on the results of an anti-parasite antibody test and examination of the larval DNA. Furthermore, serology revealed a positive test for antibodies against human T-cell leukemia virus type 1 (HTLV-1). Immunosuppressive treatment was terminated; ivermectin was administered, which resulted in improvements in colitis symptoms within a few weeks. There are several published reports describing Strongyloides stercoralis colitis as a lethal mimic of UC. Corticosteroid and anti-TNF therapies have been reported as among the major risk factors associated with strongyloidiasis in patients with HTLV-1 infection. Therefore, HTLV-1 and Strongyloides infections may be considered in cases of new-onset gastrointestinal symptoms during immunosuppressive therapy, particularly in HTLV-1-endemic regions.
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A Case of Sparganosis Caused by Spirometra decipiens on the Femur 査読あり
Yogo S., Imamura S., Nagai K., Harada K., Nagayasu E., Maruyama H., Urabe K.
Nishinihon Journal of Dermatology 83 ( 5 ) 453 - 456 2021年
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Nishinihon Journal of Dermatology
63 歳，女性。初診の 3 カ月程前から出現した左大腿の皮下結節を主訴に当科を紹介され受診した。初診時，左大腿内側に紫斑とその直下に 1 cm 程の皮下硬結を触れ，超音波検査では肉芽腫が疑われた。切除生検を行ったところ，病理組織検査で寄生虫が確認され，類結核性肉芽腫が真皮から皮下にかけてみられた。寄生虫は，DNA 検査の結果 <i>Spirometra decipiens</i> と同定された。<i>Spirometra decipiens</i> は，マンソン孤虫症の原因であるマンソン裂頭条虫とは異なる孤虫であり，今後広く認知されることが期待される。
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Aye KM, Nagayasu E, Nyunt MH, Zaw NN, Thant KZ, Kyaw MP, Maruyama H
BMC infectious diseases 20 ( 1 ) 906 - 906 2020年11月
担当区分：最終著者, 責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：BMC Infectious Diseases
Backgrounds: Primary infection with Toxoplasma gondii during pregnancy can pose serious health problems for the fetus. However, the epidemiological status of toxoplasmosis among reproductive-aged population in Myanmar is largely unknown. Although luciferase immunoprecipitation system (LIPS) assays for serodiagnosis of toxoplasmosis was developed mostly using mouse infection model, had not been tested by using field-derived human samples. Methods: A total of 251 serum samples were collected from reproductive-aged women, residing in Shwegyin township, Bago region, Myanmar and analyzed with a commercial ELISA kit, as well as in-house LIPS assays. Results: The overall seroprevalence for Toxoplasma gondii infection by the commercial ELISA was 11.5%. No clear risk factor was identified except for being in the younger age group (15–30 years old). Overall, LIPS assays showed low sensitivity when the commercial ELSA was used as a reference test. Conclusion: We identified the epidemiological situation of toxoplasmosis in some rural communities in Myanmar. The data obtained here will serve as a primary information for the effort to reduce toxoplasmosis in this region. Although looked promising in the previous experiments with mouse infection model, we found that the reported LIPS procedures need further improvements to increase the sensitivities.
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Phylogenetic relationships of Strongyloides species in carnivore hosts. 査読あり 国際共著 国際誌
Ko PP, Suzuki K, Canales-Ramos M, Aung MPPTHH, Htike WW, Yoshida A, Montes M, Morishita K, Gotuzzo E, Maruyama H, Nagayasu E
Parasitology international 78 102151 - 102151 2020年10月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Parasitology International
Strongyloides stercoralis is a parasitic nematode and a major pathogen responsible for human strongyloidiasis. The presence of this species in the dog population has led to an interest in studying the phylogenetic relationships among Strongyloides spp. in carnivore hosts. In the present study, Strongyloides spp. from various carnivore hosts (raccoon, Japanese badger, Siberian weasel, raccoon dog, masked palm civet, and domestic cat) were sought. Except for civets, Strongyloides spp. were identified in all host species. Based on 18S rDNA sequences, nine OTUs (operational taxonomy units) were identified. Molecular phylogenetic analyses using 18S[sbnd]28S rDNA and mitochondrial cox1 (cytochrome c oxidase subunit 1) sequences clustered them into two groups. The first group (named the stercoralis/procyonis group) was comprised of six OTUs and occurred in cats, raccoon dogs, raccoons (S. procyonis), Siberian weasels, and Japanese badgers and included S. stercoralis from humans and dogs. The second group (named the planiceps group) was made up of Strongyloides spp. from raccoon dogs (two OTUs) and one OTU from Siberian weasels. Subsequent analysis using almost the full-length nucleotide sequences of protein-coding genes in their mitochondrial genomes placed Strongyloides spp. of cats in a sister taxon position to S. stercoralis, whereas S. procyonis from raccoons was more distantly related to them. The presence of Strongyloides spp. from various carnivore hosts, which are close relatives of S. stercoralis, suggests this group of Strongyloides (the stercoralis/procyonis group) essentially evolved as parasites of carnivores, although more data on Strongyloides spp. from primate hosts are needed.
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A case of seronegative longitudinally extensive transverse myelitis with possible neuro sweet disease. 査読あり
Toko M, Nezu T, Ueno H, Kamimura T, Aoki S, Hosomi N, Maruyama H
eNeurologicalSci 18 100227 2020年3月
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我が国における妊婦マラリアの検討 査読あり
古賀 道子, 木村 幹男, 長谷川 千尋, 武藤 義和, 忽那 賢志, 加藤 康幸, 丸山 治彦
感染症学雑誌 94 ( 2 ) 254 - 255 2020年3月
担当区分：最終著者 記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：(一社)日本感染症学会
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Comparison of a 22-gauge Franseen-tip needle with a 20-gauge forward-bevel needle for the diagnosis of type 1 autoimmune pancreatitis: a prospective, randomized, controlled, multicenter study (COMPAS study).
Kurita A, Yasukawa S, Zen Y, Yoshimura K, Ogura T, Ozawa E, Okabe Y, Asada M, Nebiki H, Shigekawa M, Ikeura T, Eguchi T, Maruyama H, Ueki T, Itonaga M, Hashimoto S, Shiomi H, Minami R, Hoki N, Takenaka M, Itokawa Y, Uza N, Hashigo S, Yasuda H, Takada R, Kamada H, Kawamoto H, Kawakami H, Moriyama I, Fujita K, Matsumoto H, Hanada K, Takemura T, Yazumi S
Gastrointestinal endoscopy 91 ( 2 ) 373 - 381.e2 2020年2月
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寄生虫症抗体検査のこれまでとこれから
丸山治彦
Clinical Parasitology 2019年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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寄生虫症抗体検査のこれまでとこれから
丸山治彦
Clinical Parasitology 2019年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Establishment of a serodiagnosis system for the detection of Toxocara spp. and Ascaris suum infection in chickens. 査読あり 国際共著 国際誌
Nguyen YTH, Hayata Y, Sonoda S, Nonaka N, Maruyama H, Yoshida A.
Parasitology International 75 102022 - 102022 2019年11月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Parasitology International
Chickens are considered to act as paratenic hosts for agents, Toxocara canis, T. cati and Ascaris suum; which cause ascarid larva migrans syndrome (ascarid LMS) in humans. In addition, they are the definitive host for Ascaridia galli, considered not to be infective for humans. All ascarid parasites can have a high homology of antigenicity, leading to cross-reactivity in serodiagnostic assays. This study was conducted to establish a procedure for the serological detection of those roundworm infections in chickens. Twenty-five male Julia chickens were divided into five groups (n = 5); T. canis-, T. cati-, Ascaris suum- and Ascaridia galli-infected, and an uninfected control group. In Ascaris suum-soluble worm antigen preparation (As-SWAP) ELISA, all infected groups showed an elevation of anti-ascarid antibodies, indicating the usefulness of As-SWAP as a screening antigen for the detection of ascarid infections. For infecting species identification, T. canis-excretory/secretory (Tc-ES) and Ascaris suum-ES (As-ES) antigen ELISA were conducted by serial dilution sera. Toxocara spp.-infected sera showed stronger binding to Tc-ES than As-ES, while Ascaris suum and Ascaridia galli-infected sera bound to As-ES more strongly than Tc-ES. To discriminate between Ascaris suum and Ascaridia galli infection, sera were pre-incubated with Ascaridia galli-SWAP antigen and applied to Tc-ES and As-ES ELISAs. In this pre-adsorbed ES antigen ELISAs, only the Ascaris suum infected group showed positive binding to As-ES, resulting from the adsorption of cross-reactive antibodies in Ascaridia galli-infected sera. Finally, anti-Toxocara specific antibodies were confirmed by Tc-ES western blot (WB). Toxocara spp.-infected sera showed toxocariasis-specific band pattern in Tc-ES WB, while no specific band appeared on any strip incubated with Ascaris suum, Ascaridia galli-infected and uninfected sera. In conclusion, the serodiagnostic assays evaluated in this study are useful for the detection of ascarid infections in chickens.
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Paragonimus and paragonimiasis in Asia: An update. 査読あり
Yoshida A, Doanh PN, Maruyama H.
Acta Tropica 2019年11月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Characterization of the RAGE-binding protein, Strongyloides venestatin, produced by the silkworm-baculovirus expression system. 査読あり
Tsubokawa D, Lee JM, Hatta T, Mikami F, Maruyama H, Arakawa T, Kusakabe T, Tsuji N.
Infection, Genetics and Evolution 2019年11月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Improved 18S and 28S rDNA primer sets for NGS-based parasite detection. 査読あり
Kounosu A, Murase K, Yoshida A, Maruyama H, Kikuchi T.
Scientific Reports 2019年10月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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First molecular identification of Strongyloides vituli in cattle in Japan and insights into the evolutionary history of Strongyloides parasites of ruminants. 査読あり
Ko, P.P., Sakaguchi, K., Yoshida, A., Maruyama, H., Nonaka, N., Nagayasu E.
Parasitology International 2019年10月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Sequential Changes in the Host Gut Microbiota During Infection With the Intestinal Parasitic Nematode Strongyloides venezuelensis. 査読あり
Afrin T., Murase K., Kounosu A., Hunt V., Bligh M., Maeda Y., Hino A., Maruyama H., Tsai I., Kikuchi T.
Frontiers in Cellular and Infection Microbiology 2019年6月
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Maeda Y., Palomares-Rius J., Hino A., Afrin T., Mondal S., Nakatake A., Maruyama H., Kikuchi T.
Parasites and Vectors 12 ( 1 ) 21 2019年1月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Parasites and Vectors
© 2019 The Author(s). Background: Parasites excrete and secrete a wide range of molecules that act as the primary interface with their hosts and play critical roles in establishing parasitism during different stages of infection. Strongyloides venezuelensis is a gastrointestinal parasite of rats that is widely used as a laboratory model and is known to produce both soluble and insoluble (adhesive) secretions during its parasitic stages. However, little is known about the constituents of these secretions. Results: Using mass spectrometry, we identified 436 proteins from the infective third-stage larvae (iL3s) and 196 proteins from the parasitic females of S. venezuelensis. The proteins that were secreted by the iL3s were enriched with peptidase activity, embryo development and the oxidation-reduction process, while those of the parasitic females were associated with glycolysis, DNA binding (histones) and other unknown functions. Trypsin inhibitor-like domain-containing proteins were identified as the main component of the adhesive secretion from parasitic females. An absence of secretion signals in many of the proteins indicated that they are secreted via non-classical secretion pathways. Conclusions: We found that S. venezuelensis secretes a wide range of proteins to establish parasitism. This includes proteins that have previously been identified as being involved in parasitism in other helminths as well as proteins that are unique to this species. These findings provide insights into the molecular mechanisms underlying Strongyloides parasitism.
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Prevalence and associated risk factors of Strongyloides stercoralis infection in Lower Myanmar. 査読あり
Aung M., Hino A., Oo K., Win K., Maruyama H., Htike W., Nagayasu E.
Tropical medicine and Health 46 ( 1 ) 43 2018年12月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Tropical Medicine and Health
© 2018 The Author(s). Background: Strongyloidiasis is prevalent in Southeast Asian regions along with other soil-transmitted helminthiases, but only limited present-day data was available for Myanmar. Methods: A prevalence survey for Strongyloides stercoralis infection was conducted among villagers in rural areas of three townships located in the Lower Myanmar during 2014-2016 by agar plate culture method in combination with specific identification by molecular assays. Risk factors associated with S. stercoralis infection were assessed by analyzing questionnaires obtained from study participants. Results: Strongyloides stercoralis was identified in 40 out of 703 participants (5.7% overall prevalence). The highest prevalence (14.4%) was observed in Htantabin, while other two communities (Thabaung and Thanlyin) had much lower prevalence (2.2 and 2.5%, respectively). Infection was relatively rare (1.2%) in younger generations under 20 years compared to older generations (9.5%). Even in Htantabin, none of the female residents under age 40 (n = 33) had infection. In adult Htantabin residents, those who answered that they do not wear shoes regularly had an elevated risk of infection (odds ratio = 2.50, 95% confidence interval = 1.03-6.08). Conclusions: This study showed that there is still an on-going transmission of strongyloidiasis in Lower Myanmar. It is highly desirable that the soil should be free of fecal contamination by improving the management of fecal waste. Meanwhile, health education to promote shoe-wearing would be beneficial to reduce the risk of transmission, especially for those who have frequent and intense contact with soil.
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ヒトとイヌに由来するStrongyloides stercoralisの分子系統解析
長安英治, 丸山治彦
Clinical Parasitology 2018年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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ヒトとイヌに由来するStrongyloides stercoralisの分子系統解析
長安英治, 丸山治彦
Clinical Parasitology 2018年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌）