論文 - 丸山 治彦
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症例 抗顎口虫抗体陽性で診断,イベルメクチン内服が奏効したCreeping Diseaseの1例
齋藤 麗子, 太田 瑞穂, 冲津 成実, 徳山 道生, 近藤 章生, 栄枝 重典, 橘 裕司, 石井 則久, 丸山 治彦, 馬渕 智生
皮膚科の臨床 64 ( 6 ) 1129 - 1133 2022年5月
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[Current real-world treatments for Japanese patients with eosinophilic esophagitis].
Fujiwara Y, Tanaka F, Nakata R, Kakiya Y, Yamamura M, Tanoue K, Sawada A, Higashimori A, Nishida Y, Maruyama H, Ominami M, Nadatani Y, Fukunaga S, Otani K, Hosomi S, Kamata N, Nagami Y, Taira K
Nihon Shokakibyo Gakkai zasshi = The Japanese journal of gastro-enterology 119 ( 10 ) 929 - 936 2022年
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大腿に生じたSpirometra decipiensによる孤虫症の1例 査読あり
與語 晶子, 今村 桜子, 永井 貴子, 原田 佳代, 長安 英治, 丸山 治彦, 占部 和敬
西日本皮膚科 83 ( 5 ) 477 - 477 2021年10月
記述言語：日本語 掲載種別：症例報告 出版者・発行元：日本皮膚科学会-西部支部
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Takaki Y, Kadekaru S, Takami Y, Yoshida A, Maruyama H, Une Y, Nagayasu E
Parasitology international 84 102399 2021年10月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Parasitology International
Strongyloides is a genus of parasitic nematodes of vertebrates that contains over 50 species, each with a variable host range. A recent molecular phylogenetic analysis on this genus showed that Strongyloides spp. from various carnivore hosts form a strongly supported clade together with Strongyloides stercoralis, a major pathogen of humans and dogs (named the “stercoralis/procyonis group”). In the present study, we obtained DNA sequencing data of Strongyloides sp. isolated from an imported meerkat (Suricata suricatta). Based on the phylogenetic analysis, we considered this a new member of the stercoralis/procyonis group. This study represents the first isolation and molecular characterization of a Strongyloides species from hosts belonging to the family Herpestidae (mongooses and meerkat). However, whether the meerkat serves as a natural host of this Strongyloides species remains to be investigated.
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Clinico-radiologic Characteristics of Pulmonary Visceral Larva Migrans Caused by Ascaris suum. 査読あり
Matsumoto N, Tsubouchi H, Setoguchi K, Horiguchi T, Shigekusa T, Tsuchida S, Matsuo A, Oda Y, Yanagi S, Maruyama H, Nakazato M
Internal medicine (Tokyo, Japan) 60 ( 18 ) 2899 - 2903 2021年9月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Internal Medicine
Objective Visceral larva migrans (VLM) caused by Ascaris suum is a major health problem in pig farming regions. The clinical characteristics of pulmonary VLM caused by A. suum, however, are unclear. We assessed the clinico-radiologic features of this disease. Methods Medical records, including the results of chest radiography and high-resolution computed tomography (HRCT), were retrospectively reviewed from January 2000 through June 2019, at the University of Miyazaki Hospital and Kyoritsuiin Hospital in Miyazaki Prefecture, Japan. Results Seven patients with VLM caused by A. suum were identified. All seven patients had a unique habit of consuming raw foods, such as organic vegetables, chicken, turkey, wild boar, and venison. All but one patient, who had eosinophilic pneumonia with a fever and severe fatigue, had only mild or no respiratory symptoms. All 7 patients had remarkable eosinophilia (median, 1,960/μL) and high serum IgE levels (median, 1,346 IU/mL). Chest HRCT revealed multiple nodules and multiple nodular ground-glass opacities in 57% and 29% of the patients, respectively. The pulmonary lesions were located predominantly in subpleural areas. All seven patients were treated with albendazole, which led to improvement within two to three months. Neither eggs nor parasites were detected in the feces or sputum of any patient. Conclusion Consumption of raw organic vegetables or raw meat is a possible route of A. suum infection. Infected patients exhibit mild respiratory symptoms, and multiple nodules with a halo in the subpleural area are a common finding on chest HRCT. Treatment with albendazole was effective in these cases.
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Tanaka M, Kildemoes AO, Chadeka EA, Cheruiyot BN, Sassa M, Moriyasu T, Nakamura R, Kikuchi M, Fujii Y, de Dood CJ, Corstjens PLAM, Kaneko S, Maruyama H, Njenga SM, de Vrueh R, Hokke CH, Hamano S
Parasitology international 83 102346 2021年8月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Parasitology International
Schistosomiasis remains a worldwide public health problem, especially in sub-Saharan Africa. The World Health Organization targets the goal for its elimination as a public health problem in the 2030 Neglected Tropical Diseases (NTDs) Roadmap. Concerted action and agile responses to challenges will be necessary to achieve the targets. Better diagnostic tests can accelerate progress towards the elimination by monitoring disease trends and evaluating the effectiveness of interventions; however, current examinations such as Kato–Katz technique are of limited power to detect light-intensity infections. The point-of-care circulating cathodic antigen (POC-CCA) test shows a higher sensitivity compared to the reference standard, Kato-Katz technique, but it still lacks sufficient sensitivity with low infection intensity. In this study, we examined antibody reactions against recombinant protein antigens; Schistosoma mansoni serine protease-inhibitor (SmSerpin) and RP26, by enzyme-linked immunosorbent assay (ELISA) in plasma samples with light-intensity infection. The sensitivity using the cocktail antigen of recombinant SmSerpin and RP26 showed 83.7%. The sensitivity using S. mansoni soluble egg antigen (SmSEA) was 90.8%, but it showed poor specificity (29.7%), while the cocktail antigen presented improved specificity (61.4%). We conclude that antibody detection to the SmSerpin and RP26 protein antigens is effective to detect S. mansoni light-intensity infections. Our study indicates the potential of detecting antibody against recombinant protein antigens to monitor the transmission of schistosomiasis in low endemicity contexts.
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Tsubokawa D, Kikuchi T, Lee JM, Kusakabe T, Yamamoto Y, Maruyama H
PLoS pathogens 17 ( 6 ) e1009649 2021年6月
担当区分：最終著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：PLoS Pathogens
Parasitic helminths can reside in humans owing to their ability to disrupt host protective immunity. Receptor for advanced glycation end products (RAGE), which is highly expressed in host skin, mediates inflammatory responses by regulating the expression of pro-inflammatory cytokines and endothelial adhesion molecules. In this study, we evaluated the effects of venestatin, an EF-hand Ca2+-binding protein secreted by the parasitic helminth Strongyloides venezuelensis, on RAGE activity and immune responses. Our results demonstrated that venestatin bound to RAGE and downregulated the host immune response. Recombinant venestatin predominantly bound to the RAGE C1 domain in a Ca2+-dependent manner. Recombinant venestatin effectively alleviated RAGE-mediated inflammation, including footpad edema in mice, and pneumonia induced by an exogenous RAGE ligand. Infection experiments using S. venezuelensis larvae and venestatin silencing via RNA interference revealed that endogenous venestatin promoted larval migration from the skin to the lungs in a RAGE-dependent manner. Moreover, endogenous venestatin suppressed macrophage and neutrophil accumulation around larvae. Although the invasion of larvae upregulated the abundance of RAGE ligands in host skin tissues, mRNA expression levels of tumor necrosis factor-α, cyclooxygenase-2, endothelial adhesion molecules vascular cell adhesion protein-1, intracellular adhesion molecule-1, and E-selectin were suppressed by endogenous venestatin. Taken together, our results indicate that venestatin suppressed RAGE-mediated immune responses in host skin induced by helminthic infection, thereby promoting larval migration. The anti-inflammatory mechanism of venestatin may be targeted for the development of anthelminthics and immunosuppressive agents for the treatment of RAGE-mediated inflammatory diseases.
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Study Protocol for a Randomized, Double-Blind, Placebo-Controlled, Phase-II Trial: AdrenoMedullin for Ischemic Stroke Study.
Yoshimoto T, Saito S, Omae K, Hattori Y, Fukuma K, Kitamura K, Kakuta R, Kita T, Maruyama H, Yamamoto H, Ihara M
Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association 30 ( 6 ) 105761 2021年6月
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Nguyen YTH, Nonaka N, Maruyama H, Yoshida A
The Journal of veterinary medical science 83 ( 4 ) 671 - 674 2021年4月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Veterinary Medical Science
This study aimed to evaluate the sampling method for the detection of Ascaris suum larval DNA in chicken livers using real-time PCR. Chickens were inoculated with A. suum eggs of a single dose (Group A) or repeatedly low doses (Group B). White spots (WSs) were continuously observed on liver from day 3 after the last infection in Group B and day 14 in Group A. In Group A, larval DNA was detected in WS lesions (78.6%) at a significantly higher rate than in the remaining tissue samples (31.3%). In conclusion, applying WS lesions to the assay improved the detection rate of A. suum DNA in chicken livers, especially in the case of a single infection.
DOI： 10.1292/jvms.20-0404
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Abdominal paragonimiasis after consumption of wild boar meat. 査読あり 国際誌
Nakashima S, Takajo I, Maruyama H, Nagayasu E
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases 105 40 - 41 2021年4月
記述言語：英語 掲載種別：症例報告 出版者・発行元：International Journal of Infectious Diseases
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Surgically resected hepatic mass caused by fascioliasis. 査読あり
Wang H, Itoh S, Matsumoto Y, Nishie A, Kurihara T, Shimagaki T, Nagao Y, Toshima T, Harada N, Kohashi K, Oda Y, Ishigami K, Maruyama H, Yoshizumi T, Mori M
Clinical journal of gastroenterology 14 ( 2 ) 662 - 667 2021年4月
記述言語：英語 掲載種別：症例報告 出版者・発行元：Clinical Journal of Gastroenterology
Fascioliasis is a parasitic infestation caused by the digenetic trematodes Fasciola hepatica and F. gigantica. It is not commonly seen in developed countries, so diagnosis there is always difficult as a result of confusion with other hepatic or biliary disorders. A 56-year-old man presented at our hospital with a hepatic mass that had been inadvertently discovered by ultrasonography. Abdominal computed tomography revealed a multi-cystic lesion distributed along the branch of the right bile duct. Endoscopic retrograde cholangiopancreatography showed serrated changes ranging from the upper level of the common bile duct to the right hepatic bile duct. Eosinophilia was not observed and tumor marker levels were within normal ranges. Following right lobectomy combined with bile duct reconstruction, a histological examination revealed cholangitis with inflammatory cell infiltration accompanied by parasite egg-like structures and Charcot–Leyden crystals. An additional serologic test was positive for F. hepatica antibodies. A diagnosis of fascioliasis was thus confirmed by histopathology and serology. Fascioliasis should be suspected if imaging findings such as multiple small hypodense lesions in the liver are observed, and serologic tests can be useful for differential diagnosis.
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<i>Strongyloides stercoralis</i> colitis in a patient positive for human T-cell leukaemia virus with rheumatoid arthritis during an anti-rheumatic therapy: a case report. 査読あり 国際誌
Hashiba Y, Umekita K, Minami H, Kawano A, Nagayasu E, Maruyama H, Hidaka T, Okayama A
Modern rheumatology case reports 5 ( 1 ) 16 - 21 2021年1月
記述言語：英語 掲載種別：研究論文（学術雑誌）
An elderly woman with rheumatoid arthritis (RA) presented with a chief complaint of abdominal pain and diarrhea while undergoing treatment with low-dose corticosteroids and abatacept. Endoscopic and histopathological findings revealed manifestations of ulcerative colitis (UC). An intermediate dose of corticosteroids and 5-aminosalicylic acid were administered. Abatacept was discontinued; the anti-TNF biologic, golimumab, was administered for treatment of both RA and UC. However, colitis worsened in response to this therapeutic regimen. Colonoscopy revealed severe mucosal lesions; larvae were detected in samples taken from multiple shallow mucosal ulcers. The patient was diagnosed with Strongyloides stercoralis colitis based on the results of an anti-parasite antibody test and examination of the larval DNA. Furthermore, serology revealed a positive test for antibodies against human T-cell leukemia virus type 1 (HTLV-1). Immunosuppressive treatment was terminated; ivermectin was administered, which resulted in improvements in colitis symptoms within a few weeks. There are several published reports describing Strongyloides stercoralis colitis as a lethal mimic of UC. Corticosteroid and anti-TNF therapies have been reported as among the major risk factors associated with strongyloidiasis in patients with HTLV-1 infection. Therefore, HTLV-1 and Strongyloides infections may be considered in cases of new-onset gastrointestinal symptoms during immunosuppressive therapy, particularly in HTLV-1-endemic regions.
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A Case of Sparganosis Caused by Spirometra decipiens on the Femur 査読あり
Yogo S., Imamura S., Nagai K., Harada K., Nagayasu E., Maruyama H., Urabe K.
Nishinihon Journal of Dermatology 83 ( 5 ) 453 - 456 2021年
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Nishinihon Journal of Dermatology
63 歳，女性。初診の 3 カ月程前から出現した左大腿の皮下結節を主訴に当科を紹介され受診した。初診時，左大腿内側に紫斑とその直下に 1 cm 程の皮下硬結を触れ，超音波検査では肉芽腫が疑われた。切除生検を行ったところ，病理組織検査で寄生虫が確認され，類結核性肉芽腫が真皮から皮下にかけてみられた。寄生虫は，DNA 検査の結果 <i>Spirometra decipiens</i> と同定された。<i>Spirometra decipiens</i> は，マンソン孤虫症の原因であるマンソン裂頭条虫とは異なる孤虫であり，今後広く認知されることが期待される。
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Aye KM, Nagayasu E, Nyunt MH, Zaw NN, Thant KZ, Kyaw MP, Maruyama H
BMC infectious diseases 20 ( 1 ) 906 - 906 2020年11月
担当区分：最終著者, 責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：BMC Infectious Diseases
Backgrounds: Primary infection with Toxoplasma gondii during pregnancy can pose serious health problems for the fetus. However, the epidemiological status of toxoplasmosis among reproductive-aged population in Myanmar is largely unknown. Although luciferase immunoprecipitation system (LIPS) assays for serodiagnosis of toxoplasmosis was developed mostly using mouse infection model, had not been tested by using field-derived human samples. Methods: A total of 251 serum samples were collected from reproductive-aged women, residing in Shwegyin township, Bago region, Myanmar and analyzed with a commercial ELISA kit, as well as in-house LIPS assays. Results: The overall seroprevalence for Toxoplasma gondii infection by the commercial ELISA was 11.5%. No clear risk factor was identified except for being in the younger age group (15–30 years old). Overall, LIPS assays showed low sensitivity when the commercial ELSA was used as a reference test. Conclusion: We identified the epidemiological situation of toxoplasmosis in some rural communities in Myanmar. The data obtained here will serve as a primary information for the effort to reduce toxoplasmosis in this region. Although looked promising in the previous experiments with mouse infection model, we found that the reported LIPS procedures need further improvements to increase the sensitivities.
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Phylogenetic relationships of Strongyloides species in carnivore hosts. 査読あり 国際共著 国際誌
Ko PP, Suzuki K, Canales-Ramos M, Aung MPPTHH, Htike WW, Yoshida A, Montes M, Morishita K, Gotuzzo E, Maruyama H, Nagayasu E
Parasitology international 78 102151 - 102151 2020年10月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Parasitology International
Strongyloides stercoralis is a parasitic nematode and a major pathogen responsible for human strongyloidiasis. The presence of this species in the dog population has led to an interest in studying the phylogenetic relationships among Strongyloides spp. in carnivore hosts. In the present study, Strongyloides spp. from various carnivore hosts (raccoon, Japanese badger, Siberian weasel, raccoon dog, masked palm civet, and domestic cat) were sought. Except for civets, Strongyloides spp. were identified in all host species. Based on 18S rDNA sequences, nine OTUs (operational taxonomy units) were identified. Molecular phylogenetic analyses using 18S[sbnd]28S rDNA and mitochondrial cox1 (cytochrome c oxidase subunit 1) sequences clustered them into two groups. The first group (named the stercoralis/procyonis group) was comprised of six OTUs and occurred in cats, raccoon dogs, raccoons (S. procyonis), Siberian weasels, and Japanese badgers and included S. stercoralis from humans and dogs. The second group (named the planiceps group) was made up of Strongyloides spp. from raccoon dogs (two OTUs) and one OTU from Siberian weasels. Subsequent analysis using almost the full-length nucleotide sequences of protein-coding genes in their mitochondrial genomes placed Strongyloides spp. of cats in a sister taxon position to S. stercoralis, whereas S. procyonis from raccoons was more distantly related to them. The presence of Strongyloides spp. from various carnivore hosts, which are close relatives of S. stercoralis, suggests this group of Strongyloides (the stercoralis/procyonis group) essentially evolved as parasites of carnivores, although more data on Strongyloides spp. from primate hosts are needed.
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Morphologically and Genetically Diagnosed Dermal Dioctophyme Larva in a Chinese Man: Case Report 査読あり
Tanaka T., Tokiwa T., Hasegawa H., Kadosaka T., Itoh M., Nagaoka F., Maruyama H., Mizuno Y., Kanoh H., Shirai N.
SN Comprehensive Clinical Medicine 2 ( 4 ) 468 - 471 2020年4月
掲載種別：研究論文（学術雑誌） 出版者・発行元：SN Comprehensive Clinical Medicine
We present a case of a nematode larva infection found in a cutaneous nodule excised from the left flank of a 48-year-old Chinese male, who was naturalized in Japan and comes and goes frequently to China and Japan. Clinical diagnosis at his first visit to a local hospital was epidermoid cyst. Pathological diagnosis of surgical specimen was cutaneous larva migrans and detailed morphological observation suggested that the worm in the subcutis was a dioctophimatid larva. DNA sequence analysis of the formalin-fixed and paraffin-embedded tissues revealed the larva as the giant kidney worm, Dioctophyme renale (Goeze, 1972).
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A case of seronegative longitudinally extensive transverse myelitis with possible neuro sweet disease. 査読あり
Toko M, Nezu T, Ueno H, Kamimura T, Aoki S, Hosomi N, Maruyama H
eNeurologicalSci 18 100227 2020年3月
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我が国における妊婦マラリアの検討 査読あり
古賀 道子, 木村 幹男, 長谷川 千尋, 武藤 義和, 忽那 賢志, 加藤 康幸, 丸山 治彦
感染症学雑誌 94 ( 2 ) 254 - 255 2020年3月
担当区分：最終著者 記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：(一社)日本感染症学会