論文 - 丸山 治彦
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Differential serodiagnostics of toxocara canis and toxocara cati - is it possible? 査読あり 国際共著
Poulsen C., Skov S., Yoshida A., Skallerup P., Maruyama H., Thamsborg S., Nejsum P.
Parasite Immunology 37 ( 4 ) 204 - 207 2015年4月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Parasite Immunology
© 2015 John Wiley & Sons Ltd. One of the most common zoonotic helminth infections is caused by species in the genus Toxocara, particularly Toxocara canis and T. cati (Syn. T. mystax). However, their relative contribution to toxocarosis in humans remains largely unknown because causative larvae are seldom recovered and uncertainties regarding the validity of existing serological assays. In this study, we used sera from a pig model experimentally infected with T. canis and T. cati to evaluate whether a Western blot could discriminate between the two species. No proteins were observed that could be used as a diagnostic tool. In addition, a heterogenic protein pattern between individual hosts was found, which was most pronounced in the T. cati-infected pigs. There is therefore an urgent need to optimize and validate current methods or develop new species-specific serological methods in order to implement appropriate control measures.
DOI： 10.1111/pim.12181
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New zoonotic cases of Onchocerca dewittei japonica (Nematoda: Onchocercidae) in Honshu, Japan. 査読あり
Uni S, Fukuda M, Otsuka Y, Hiramatsu N, Yokobayashi K, Takahashi H, Murata S, Kusatake K, Morita E, Maruyama H, Hasegawa H, Shiwaku K, Ramli R, Azirun MS, Takaoka H
Parasites & vectors 8 59 2015年1月
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Paragonimiasis in Japan: a twelve-year retrospective case review (2001-2012). 査読あり
Nagayasu E, Yoshida A, Hombu A, Horii Y, Maruyama H
Internal medicine (Tokyo, Japan) 54 ( 2 ) 179 - 86 2015年
担当区分：最終著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Internal Medicine
© 2015 The Japanese Society of Internal Medicine. All rights reserved. Objective: Paragonimiasis, or lung fluke infection, is a food-borne parasitic disease caused by infection with trematodes belonging to the genus Paragonimus. Although paragonimiasis was once considered successfully controlled in the 1970s, new cases began to emerge in the late 1980s. To apprehend the current-day situation of the re-emergent cases of paragonimiasis in Japan, we conducted a retrospective review of 443 patients who were referred to our laboratory and diagnosed as having paragonimiasis during 2001-2012. Methods: Patients were diagnosed as having paragonimiasis based primarily on immunodiagnostic methods in addition to clinical, laboratory, and radiographic findings. Patient data were extracted from consultation sheets from attending physicians and were analyzed. Results: Majority of the patients were residents of Kyushu Island. However, a substantial number of cases were also from other parts of Japan. Immigrants (mostly from China, Thailand, and Korea) accounted for a quarter of the cases. Native Japanese contracted paragonimiasis by consuming wild boar meat or freshwater crabs, whereas immigrants contracted the infection almost exclusively by consumption of freshwater crabs. Eosinophilia and elevated serum IgE levels were found in around 80% of the patients. Parasite egg detection was documented only in 11.7% of the cases, showing the reliance on serological tests for diagnosing paragonimiasis in current clinical practice. Conclusion: Paragonimiasis remains a public health issue in Japan, and the situation should be closely monitored.
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Kodama M, Akaki M, Tanaka H, Maruyama H, Nagayasu E, Yokouchi T, Arimura Y, Kataoka H
Journal of medical case reports 8 ( 1 ) 346 2014年10月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Medical Case Reports
© 2014 Al-Kandari et al.; licensee BioMed Central Ltd. Introduction: Paragonimiasis is a food-borne infection caused by Paragonimus parasites. The lungs and pleura are the primary sites for the infection; however, ectopic infection can occur in other organs such as skin, liver and brain. It is difficult to make a diagnosis of ectopic paragonimiasis due to an ignorance of, and unfamiliarity with the disease. We report the case of a patient with subcutaneous paragonimiasis diagnosed by histopathological analysis and serological testing. Case presentation: A 39-year-old Chinese immigrant woman presented with a subcutaneous nodule in her left lower back. The nodule was initially suspected of lipoma and she was followed up on without any treatment. However, it gradually indurated and the nodule was resected surgically. A magnetic resonance imaging scan revealed a polycystic lesion with inhomogeneous low or high intensity on T1-or T2-weighted images, respectively. The rim of the lesion was enhanced after contrast enhancement, but the inside did not show high-signal intensity. A histological analysis of the surgically resected specimen revealed variable-sized tubulo-cystic structures. The cyst wall showed a granulomatous change with scant eosinophilic infiltration. A number of parasite ova were observed in the necrotic tissue inside the cysts, and a parasite body with a presumed oral sucker and reproductive organ was also detected, suggesting a trematode infection. A subsequent serological examination showed a positive reaction of her serum to the Paragonimus westermani antigen. No abnormal findings were found on her chest computed tomography scan. The diagnosis of subcutaneous paragonimiasis caused by Paragonimus westermani was made. Conclusions: We report a case presenting only as a non-migratory subcutaneous nodule without any pleuropulmonary lesion, which was initially suspected of lipoma but denied by magnetic resonance imaging scan results. The case was subsequently diagnosed as subcutaneous paragonimiasis from the results of histopathological analysis and serological testing.
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Efficient de novo assembly of highly heterozygous genomes from whole-genome shotgun short reads.
Kajitani R, Toshimoto K, Noguchi H, Toyoda A, Ogura Y, Okuno M, Yabana M, Harada M, Nagayasu E, Maruyama H, Kohara Y, Fujiyama A, Hayashi T, Itoh T
Genome research 24 ( 8 ) 1384 - 95 2014年8月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Genome Research
Although many de novo genome assembly projects have recently been conducted using high-throughput sequencers, assembling highly heterozygous diploid genomes is a substantial challenge due to the increased complexity of the de Bruijn graph structure predominantly used. To address the increasing demand for sequencing of nonmodel and/or wildtype samples, in most cases inbred lines or fosmid-based hierarchical sequencing methods are used to overcome such problems. However, these methods are costly and time consuming, forfeiting the advantages of massive parallel sequencing. Here, we describe a novel de novo assembler, Platanus, that can effectively manage high-throughput data from heterozygous samples. Platanus assembles DNA fragments (reads) into contigs by constructing de Bruijn graphs with automatically optimized k-mer sizes followed by the scaffolding of contigs based on paired-end information. The complicated graph structures that result from the heterozygosity are simplified during not only the contig assembly step but also the scaffolding step. We evaluated the assembly results on eukaryotic samples with various levels of heterozygosity. Compared with other assemblers, Platanus yields assembly results that have a larger scaffold NG50 length without any accompanying loss of accuracy in both simulated and real data. In addition, Platanus recorded the largest scaffold NG50 values for two of the three low-heterozygosity species used in the de novo assembly contest, Assemblathon 2. Platanus therefore provides a novel and efficient approach for the assembly of gigabase-sized highly heterozygous genomes and is an attractive alternative to the existing assemblers designed for genomes of lower heterozygosity. © 2014 Kajitani et al.
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A 71-year-old Man from Japan with Eosinophilia and a Nodular Lesion in the Lung: Paragonimiasis
Nawa Y., Maruyama H., Tomita M.
Clinical Cases in Tropical Medicine 268 - 272 2014年5月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Clinical Cases in Tropical Medicine
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Assessment of helminth biodiversity in wild rats using 18S rDNA based metagenomics. 査読あり 国際共著
Tanaka R, Hino A, Tsai IJ, Palomares-Rius JE, Yoshida A, Ogura Y, Hayashi T, Maruyama H, Kikuchi T
PloS One 9 ( 10 ) e110769 2014年
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：PLoS ONE
© 2014 Tanaka et al. Parasite diversity has important implications in several research fields including ecology, evolutionary biology and epidemiology. Wide-ranging analysis has been restricted because of the difficult, highly specialised and time-consuming processes involved in parasite identification. In this study, we assessed parasite diversity in wild rats using 18S rDNA-based metagenomics. 18S rDNA PCR products were sequenced using an Illumina MiSeq sequencer and the analysis of the sequences using the QIIME software successfully classified them into several parasite groups. The comparison of the results with those obtained using standard methods including microscopic observation of helminth parasites in the rat intestines and PCR amplification/sequencing of 18S rDNA from isolated single worms suggests that this new technique is reliable and useful to investigate parasite diversity.
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Kanai S., Nakamura T., Nagato M., Taniguchi M., Nakamura I., Kawaguchi A., Shimomatsuya T., Maruhashi K., Kusui T., Maruyama H.
Japanese Journal of Gastroenterological Surgery 47 ( 5 ) 281 - 289 2014年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Japanese Journal of Gastroenterological Surgery
A 73-year-old man who consulted our hospital with a chief complaint of upper abdominal pain was admitted with a diagnosis of acute pancreatitis. An image of a localized irregular narrow segment was observed in the main pancreatic duct of the pancreatic head upon ERCP, with a poorly contrasting region measuring 8 mm in size, corresponding to the narrow segment observed on abdominal CT. A blocked main pancreatic duct and common bile duct were observed in the vicinity of the pancreaticobiliary duct upon MRCP. Based on the laboratory finding, pancreaticoduodenectomy and right nephrectomy were performed with a preoperative diagnosis of pancreatic cancer and right renal cell cancer. As no malignant findings were observed in the resected specimen, and only plant tissue was found in the main pancreatic duct in histopathological laboratory findings, it was diagnosed as obstructive pancreatitis caused by a foreign substance in the main pancreatic duct. The mechanism of the appearance of the foreign substance in the main pancreatic duct was unknown in this case because the patient had no history of drug use that was likely to influence the function of the papillary sphincter muscle or a history of choledocholithiasis, and because no evident morphological defects in the duodenal papilla were found through imaging.© 2014 The Japanese Society of Gastroenterological Surgery.
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動物由来回虫症に対するアルベンダゾールの有効性の検討
本部エミ、吉田彩子、長安英治、黒木美香、丸山治彦
Clinical Parasitology 24 ( 1 ) 91 - 93 2013年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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寄生虫症血清診断から見た肺吸虫症の最近の動向
長安英治、吉田彩子、本部エミ、黒木美香、丸山治彦
Clinical Parasitology 24 ( 1 ) 100 - 102 2013年12月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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動物由来回虫症に対するアルベンダゾールの有効性の検討
本部エミ、吉田彩子、長安英治、黒木美香、丸山治彦
Clinical Parasitology 24 ( 1 ) 91 - 93 2013年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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寄生虫症血清診断から見た肺吸虫症の最近の動向
長安英治、吉田彩子、本部エミ、黒木美香、丸山治彦
Clinical Parasitology 24 ( 1 ) 100 - 102 2013年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Efficacy and safety of paromomycin for treating amebiasis in Japan. 査読あり
Kikuchi T, Koga M, Shimizu S, Miura T, Maruyama H, Kimura M.
Parasitology International 62 ( 6 ) 497 - 501 2013年7月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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The role of B-cells in immunity against adult Strongyloides venezuelensis. 査読あり
El-Malky MA, Maruyama H, Al-Harthi SA, El-Beshbishi SN, Ohta N.
Parasites & Vectors 24 ( 6 ) 148 2013年5月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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山川 英晃, 高柳 昇, 石黒 卓, 多田 麻美, 丸山 治彦, 杉田 裕
日本呼吸器学会誌 = Annals of the Japanese Respiratory Society 2 ( 3 ) 289 - 294 2013年5月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：日本呼吸器学会
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Identification of a bacteria-like ferrochelatase in Strongyloides venezuelensis, an animal parasitic nematode. 査読あり
Nagayasu E, Ishikawa SA, Taketani S, Chakraborty G, Yoshida A, Inagaki Y, Maruyama H.
PLoS One 8 ( 3 ) e58458 2013年3月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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ke ferrochelatase in Strongyloides venezuelensis, an animal parasitic nematode. 査読あり
Nagayasu E, Ishikawa SA, Taketani S, Chakraborty G, Yoshida A, Inagaki Y, Maruyama H
PLoS One 8 ( 3 ) 58458 - 58458 2013年3月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Transcriptomic analysis of four developmental stages of Strongyloides venezuelensis. 査読あり
Nagayasu E, Ogura Y, Itoh T, Yoshida A, Chakraborty G, Hayashi T, Maruyama H
Parasitology International 62 ( 1 ) 57 - 65 2013年2月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Transcriptomic analysis of four developmental stages of Strongyloides venezuelensis.
Nagayasu E, Ogura Y, Itoh T, Yoshida A, Chakraborty G, Hayashi T, Maruyama H
Parasitology International 62 ( 1 ) 57 - 65 2013年2月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Transcriptomic analysis of four developmental stages of Strongyloides venezuelensis.
Nagayasu E, Ogura Y, Itoh T, Yoshida A, Chakraborty G, Hayashi T, Maruyama H
Parasitology international 62 ( 1 ) 57 - 65 2013年2月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Parasitology International
Strongyloides venezuelensis is one of some 50 species of genus Strongyloides, obligate gastrointestinal parasites of vertebrates, responsible for strongyloidiasis in humans and other domestic/companion animals. Although S. venezuelensis has been widely used as a model species for studying human/animal strongyloidiasis, the sequence information for this species has been quite limited. To create a more comprehensive catalogue of expressed genes for identification of genes potentially involved in animal parasitism, we conducted a de novo sequencing analysis of the transcriptomes from four developmental stages of S. venezuelensis, using a Roche 454 GS FLX Titanium pyrosequencing platform. A total of 14,573 contigs were produced after de novo assemblies of over 2. million sequencing reads and formed a dataset "Vene454". BLAST homology search of Vene454 against proteome and transcriptome data from other animal-parasitic and non-animal-parasitic nematode species revealed several interesting genes, which may be potentially related to animal parasitism, including nicotinamide phosphoribosyltransferase and ferrochelatase. The Vene454 dataset analysis also enabled us to identify transcripts that are specifically enriched in each developmental stage. This work represents the first large-scale transcriptome analysis of S. venezuelensis and the first study to examine the transcriptome of the lung L3 developmental stage of any Strongyloides species. The results not only will serve as valuable resources for future functional genomics analyses to understand the molecular aspects of animal parasitism, but also will provide essential information for ongoing whole genome sequencing efforts in this species. © 2012 Elsevier Ireland Ltd.