論文 - 丸山 治彦
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Nagayasu E., Aung M., Hortiwakul T., Hino A., Tanaka T., Higashiarakawa M., Olia A., Taniguchi T., Win S., Ohashi I., Odongo-Aginya E., Aye K., Mon M., Win K., Ota K., Torisu Y., Panthuwong S., Kimura E., Palacpac N., Kikuchi T., Hirata T., Torisu S., Hisaeda H., Horii T., Fujita J., Htike W., Maruyama H.
Scientific reports 7 ( 1 ) 4844 2017年12月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Scientific Reports
© 2017 The Author(s). Humans and dogs are the two major hosts of Strongyloides stercoralis, an intestinal parasitic nematode. To better understand the phylogenetic relationships among S. stercoralis isolates infecting humans and dogs and to assess the zoonotic potential of this parasite, we analyzed mitochondrial Cox1, nuclear 18S rDNA, 28S rDNA, and a major sperm protein domain-containing protein genes. Overall, our analyses indicated the presence of two distinct lineages of S. stercoralis (referred to as type A and type B). While type A parasites were isolated both from humans and dogs in different countries, type B parasites were found exclusively in dogs, indicating that the type B has not adapted to infect humans. These epidemiological data, together with the close phylogenetic relationship of S. stercoralis with S. procyonis, a Strongyloides parasite of raccoons, possibly indicates that S. stercoralis originally evolved as a canid parasite, and later spread into humans. The inability to infect humans might be an ancestral character of this species and the type B might be surmised to be an origin population from which human-infecting strains are derived.
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小腸アニサキス症の2例
森紘一朗，佐原利典，藤田裕晃，中村（内山）ふくみ，大西健児，丸山治彦
Clinical Parasitology 2017年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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熱帯病治療薬研究班の登録患者からみたわが国の輸入熱帯病・寄生虫症の動向
丸山治彦，加藤康幸，古賀道子，菊地 正，木村幹男，熱帯病治療薬研究班
Clinical Parasitology 2017年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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熱帯病治療薬研究班の登録患者からみたわが国の輸入熱帯病・寄生虫症の動向
丸山治彦，加藤康幸，古賀道子，菊地 正，木村幹男，熱帯病治療薬研究班
Clinical Parasitology 2017年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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小腸アニサキス症の2例
森紘一朗，佐原利典，藤田裕晃，中村（内山）ふくみ，大西健児，丸山治彦
Clinical Parasitology 2017年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Uni S, Fukuda M, Ogawa K, Lim YA, Agatsuma T, Bunchom N, Saijuntha W, Otsuka Y, Bhassu S, Mat Udin AS, Zainuri NA, Omar H, Nakatani J, Matsubayashi M, Maruyama H, Ramli R, Azirun MS, Takaoka H
Parasitology international 66 ( 5 ) 593 - 595 2017年10月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Parasitology International
© 2017 Elsevier B.V. An 11-year-old boy living in Otsu City, Shiga Prefecture, Kansai Region, Western Honshu, Japan had zoonotic onchocercosis. The patient developed a painful swelling on the little finger of his left hand. The worm detected in the excised mass had external transverse ridges but did not have inner striae in the cuticle. On the basis of the parasite's histopathological characteristics, the causative agent was identified as a female Onchocerca dewittei japonica (Spirurida: Onchocercidae). The species of the filarial parasite was confirmed by sequencing the cox1 gene of the parasite. The Japanese wild boar Sus scrofa leucomystax is a definitive host for O. dewittei japonica, which is then transmitted by blackflies as the vector to humans. The current case described occurred in the Kansai Region, Western Honshu, where such infections were previously not reported.
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糞線虫症研究の新展開
長安英治, 菊池泰生, 丸山治彦
医学と薬学 2017年10月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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糞線虫症研究の新展開
長安英治, 菊池泰生, 丸山治彦
医学と薬学 2017年10月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Imported malaria in pregnant women experienced in Japan. 査読あり
Kimura M, Koga M, Hasegawa C, Mutoh Y, Kato Y, Maruyama H.
J Infect Chemother. 23 ( 8 ) 545 - 549 2017年8月
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Daigo Tsubokawa, Takeshi Hatta, Taisei Kikuchi, Hiroki Maeda, Fusako Mikami, M Abdul Alim, Haruhiko Maruyama, Naotoshi Tsuji
International journal for parasitology 47 ( 8 ) 501 - 509 2017年7月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：International Journal for Parasitology
© 2017 Australian Society for Parasitology The secretory EF-hand Ca ++ -binding proteins act as calcium signaling molecules for control of cell functions, but those proteins from parasitic helminths are poorly understood. Here, we have identified and characterized an EF-hand Ca ++ -binding protein from the rodent nematode, Strongyloides venezuelensis, termed ‘venestatin’, which is highly conserved in Strongyloides spp. Canonical two EF-hand domains and a signal peptide are present in venestatin. A gel mobility shift assay and Ruthenium red staining indicated that the recombinant venestatin possesses binding ability with Ca ++ ions. Endogenous venestatin was seemingly localized in the hypodermis and gut of the worms and was found in the excretory-secretory products. Quantitative reverse transcription-PCR data showed that venestatin-specific transcript was upregulated in the parasitic stages of S. venezuelensis, and the upregulation occurred promptly after larval invasion through the host's skin, but not in the case of in vitro incubation. Immunization of mice with recombinant venestatin caused a 55% reduction in larval migration to the lungs, and lung hemorrhaging was mild compared with non-immunized groups, suggesting that anti-venestatin sera may interfere with larval migration from skin to lung. Our results suggest that venestatin is secreted from the hypodermis and gut of S. venezuelensis, and has pivotal roles in larval migration.
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Tsuchido Y, Nakamura-Uchiyama F, Toyoda K, Iwagami M, Tochitani K, Shinohara K, Hishiya N, Ogawa T, Uno K, Kasahara K, Ouji Y, Kano S, Mikasa K, Shimizu T, Yoshikawa M, Maruyama H
The American journal of tropical medicine and hygiene 96 ( 5 ) 1185 - 1189 2017年5月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：American Journal of Tropical Medicine and Hygiene
Copyright © 2017 by The American Society of Tropical Medicine and Hygiene. Recently, reports of delayed hemolytic anemia after treatment with artemisinin and its derivatives have emerged. Here we report two cases of delayed hemolytic anemia in a patient with severe falciparum malaria after treatment with oral artemether-lumefantrine (AL). The first patient, a 20-year-old Japanese male student, was diagnosed with falciparum malaria and was administered AL. As having a high parasitemia rate (20.6%) was the only severe malaria criterion met in this case and his general condition was stable, we continued with AL treatment. Despite disappearance of malarial parasites after 4 days of AL administration, a persistent fever remained. On days 13 and 16, a diagnosis of hemolytic anemia was made (lactate dehydrogenase [LDH]: 1,466 U/L, hemoglobin [Hb] : 7.2 g/dL). A blood smear at that time revealed no parasites. He recovered naturally from delayed hemolysis. The second patient, a 27-year-old Japanese female student, was diagnosed with falciparum malaria (parasitemia: 4.5%) and treated initially with oral quinine hydrochloride and doxycycline. The following day, parasitemia increased to 7.9% and oral AL was initiated. She was discharged on day 4 after achieving parasite clearance and afebrility. However, on day 5, fever (body temperature > 38C) recurred, and on day 11, a diagnosis of hemolytic anemia was made (LDH: 712 U/L, Hb: 8.8 g/dL). A follow-up confirmed that her condition improved gradually. AL treatment of severe malaria can cause delayed hemolytic anemia. Patients should be followed up for up to 4 weeks to detect signs of hemolysis and provide appropriate symptomatic treatment.
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Nguyen, Y.T.H., Wang, Z., Maruyama, H., Horii, Y., Nonaka, N., Yoshida, A.
Journal of Food Safety 37 ( 2 ) 2017年5月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Food Safety
© 2016 Wiley Periodicals, Inc. Ascarid larva migrans syndrome (ascarid LMS) is caused by ascarid roundworms including Ascaris suum. In East Asia, ascarid LMS has been considered as a food-borne disease in adulthood who has the dietary habitat of consuming raw or lightly cooked meat and organ meats. To evaluate the potential risk of A. suum infection from these foods, Ascaris specific real-time PCR was developed. The assay could constantly detect A. suum DNA up to 10 fg. Its specificity was confirmed by non-amplification with Toxocara canis and Toxocara cati DNA. A. suum DNA could be amplified from not only a single larva but also mouse liver spiked with a larva. Moreover, the assay could detect A. suum DNA in experimentally infected mouse liver, and showed higher sensitivity than a conventional digestion method. This real-time PCR assay would be useful for detecting the A. suum larval contamination in meat and organ meats. Practical applications: It has been considered that one of the most important risk factors for ascarid LMS in humans is the consumption of raw or undercooked meat or organ meats of domestic animals infected with Ascaris suum. Thus, we developed the novel real-time PCR with the high sensitivity and specificity in order to specifically detect A. suum DNA from animal tissues. This assay can be applied to the meat inspection procedure for the identification of parasite larval contamination that may adversely impact on public health as well as on animal health and welfare.
DOI： 10.1111/jfs.12301
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Kambe, D., Takeoka, K., Ogawa, K., Doi, K., Maruyama, H., Yoshida, A., Suenaga, T., Kageyama, T.
Multiple sclerosis and related disorders 13 116 - 118 2017年4月
記述言語：英語 掲載種別：症例報告 出版者・発行元：Multiple Sclerosis and Related Disorders
© 2017 Elsevier B.V. A 53-year-old woman was admitted to the department of neurology in Tenri Hospital because of progressive thoracic myelitis a month after she had eaten uncooked bovine liver. A previous episode of right optic neuritis and a positive test for serum anti-aquaporin-4 antibodies indicated a diagnosis of neuromyelitis optica spectrum disorders. Although the patient initially recovered with the reduction of anti-aquaporin-4 antibodies during treatment with intravenous methylprednisolone infusion and plasma exchange, her neurological symptoms deteriorated soon after the completion of plasma exchange. Western blotting analysis detected anti-Toxocara canis antibodies in the serum; thus, the patient underwent oral albendazole treatment. This resulted in the alleviation of her symptoms. We therefore consider that rigorous investigation should be encouraged to detect rare pathogens including parasites in cases of treatment-resistant neuromyelitis optica spectrum disorders.
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Toxocara canis myelitis involving the lumbosacral region: a case report. 査読あり
Hiramatsu Y, Yoshimura M, Saigo R, Arata H, Okamoto Y, Matsuura E, Maruyama H, Takashima H
The journal of spinal cord medicine 40 ( 2 ) 241 - 245 2017年3月
記述言語：英語 掲載種別：症例報告 出版者・発行元：Journal of Spinal Cord Medicine
© 2015, © The Academy of Spinal Cord Injury Professionals, Inc. 2015. Context: Toxocara canis is a parasite known to cause visceral larva migrans. The infection rarely affects the central nervous system but there have been several reports of myelitis caused by visceral larva migrans due to Toxocara canis. In previous reported cases, the lesions were located in the thoracic or cervical spinal cord. To the best of our knowledge, this is the first report of a lesion involving the lumbosacral region. Findings: A 60-year-old man developed weakness and dysesthesia in the lower limbs. The symptoms resolved spontaneously, but recurred after five months. One month later, the patient developed pollakiuria and constipation. He was a dog owner and frequently ate raw chicken meat and beef liver. Sagittal T2-weighted image (T2WI) showed swelling and hyperintensity in the spinal cord from T10 to the lumbosacral region and focal nodular enhancement on the posterior segment of the lumbar spinal cord. Blood cell counts showed slight eosinophilia and elevated serum immunoglobulin E level. Cerebrospinal fluid examination showed slight pleocytosis with eosinophilia. Enzyme-linked immunosorbent assay showed high levels of anti-Toxocara antibodies in the serum and cerebrospinal fluid. In addition, confirmatory test by Western blot was positive. The patient was initially treated with intravenous methylprednisolone with slight improvement in muscle weakness. Albendazole was added with a second course of intravenous methylprednisolone. The muscle weakness in the lower limbs improved considerably, and swelling and hyperintensity on T2WI almost disappeared. Conclusion: Our results suggest that Toxocara canis myelitis cannot be discounted even if the myelitis involves the lumbosacral region.
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Kikuchi T, Hino A, Tanaka T, Aung MP, Afrin T, Nagayasu E, Tanaka R, Higashiarakawa M, Win KK, Hirata T, Htike WW, Fujita J, Maruyama H
PLoS neglected tropical diseases 10 ( 12 ) e0005253 2016年12月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：PLoS Neglected Tropical Diseases
© 2016 Kikuchi et al. The helminth Strongyloides stercoralis, which is transmitted through soil, infects 30–100 million people worldwide. S. stercoralis reproduces sexually outside the host as well as asexually within the host, which causes a life-long infection. To understand the population structure and transmission patterns of this parasite, we re-sequenced the genomes of 33 individual S. stercoralis nematodes collected in Myanmar (prevalent region) and Japan (non-prevalent region). We utilised a method combining whole genome amplification and next-generation sequencing techniques to detect 298,202 variant positions (0.6% of the genome) compared with the reference genome. Phylogenetic analyses of SNP data revealed an unambiguous geographical separation and sub-populations that correlated with the host geographical origin, particularly for the Myanmar samples. The relatively higher heterozygosity in the genomes of the Japanese samples can possibly be explained by the independent evolution of two haplotypes of diploid genomes through asexual reproduction during the auto-infection cycle, suggesting that analysing heterozygosity is useful and necessary to infer infection history and geographical prevalence.
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Optimal ELISA antigen for the diagnosis of Ascaris suum infection in humans. 査読あり
Yoshida A, Kikuchi T, Nakagaki S, Maruyama H
Parasitology research 115 ( 12 ) 4701 - 4705 2016年12月
担当区分：最終著者, 責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Parasitology Research
© 2016, Springer-Verlag Berlin Heidelberg. Ascarid nematodes, Ascaris suum, Toxocara canis and Toxocara cati, are the most important causative species of larva migrans syndrome (LMS) in humans. Although the diagnosis of ascarid LMS is generally based on serological tests, specific serological tests for A. suum infection have not been fully developed. In the present study, the sensitivity and specificity of three A. suum antigen preparations, i.e., the somatic adult worm antigen (As-SWAP), larval excretory-secretory (ES) antigens derived from infective L3 (AsiL3-ES) and larval ES from tissue migratory L3 (AsmL3-ES), were evaluated for the serodiagnosis of A. suum infection in enzyme-linked immunosorbent assay (ELISA). We found that all A. suum antigen preparations showed positive reaction to all sera from A. suum-infected mice, while only AsmL3-ES obtained 100 % detection of anti-A. suum antibodies in human visceral ascarosis patients. Comparing the reactivity of each A. suum antigen, sera from both A. suum-infected mice and human patients bound to AsiL3-ES significantly weaker than As-SWAP and AsmL3-ES. Moreover, the OD 450 values of ELISA with the A. suum antigen preparations and T. canis larval ES antigen (TciL3-ES) were compared in order to discriminate between ascarosis and toxocarosis. Linear discriminant analysis showed that diagnosis based on TciL3-ES and AsmL3-ES ELISA gave the most reliable result for the discrimination of infecting species. In conclusion, the application of AsmL3-ES antigen in ELISA can be recommended for the serodiagnosis of A. suum infection in humans.
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A novel method to assess the biodiversity of parasites using 18S rDNA Illumina sequencing; parasitome analysis method. 査読あり
Hino A, Maruyama H, Kikuchi T
Parasitology international 65 ( 5 Pt B ) 572 - 575 2016年10月
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Thoracoscopic examination of empyema in a patient with sparganosis mansoni.
Takeda K, Suzuki J, Nagai H, Watanabe K, Yokoyama A, Ando T, Suzuki J, Ohshima N, Masuda K, Tamura A, Akagawa S, Kitani M, Hebisawa A, Matsui H, Kobayashi N, Maruyama H, Ohta K
Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy 22 ( 2 ) 120 - 3 2016年2月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Infection and Chemotherapy
© 2015 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. A 27-year-old man was admitted to our hospital with right pleural effusion. He had suffered from right chest and back pain and a high fever for one week prior to the admission. He had been treated with clarithromycin without improvement. Since thoracoscopy under local anesthesia revealed purulent effusion, synechiae and fibrous septa in the thoracic cavity, synechiotomy was performed and we started antibiotic treatment with the diagnosis of acute bacterial empyema. At the same time, we also suspected parasitic infection because of massive eosinophilic infiltration in pleural effusion and his dietary history of eating raw frogs. During the course of the disease, he had an infiltration in the right lower lobe and pneumothorax. Finally, we diagnosed him with sparganosis mansoni because his serum as well as pleural effusion was positive for the binding to sparganosis mansoni plerocercoid antigen, without any positive findings in bacteriology. His pleural effusion and lung infiltration were resolved after the administration of a high-dose praziquantel. We report this rare parasitic empyema with findings by thoracoscopic examination.
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Palomares-Rius JE, Tsai IJ, Karim N, Akiba M, Kato T, Maruyama H, Takeuchi Y, Kikuchi T
BMC genomics 16 ( 1 ) 845 2015年10月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：BMC Genomics
© 2015 Palomares-Rius et al. Background: Bursaphelenchus xylophilus is an emerging pathogenic nematode that is responsible for a devastating epi demic of pine wilt disease across Asia and Europe. In this study, we report the first genome-wide variation analysis of the nematode with an aim to obtain a full picture of its diversity. Methods: We sequenced six keyB.xylophilusstrains using Illumina HiSeq sequencer. All the strains were isolated in Japan and have been widely used in previous studies.Detection of genomic variations were done by mapping the reads to the reference genome. Results: Over 3Mb of genetic variations, accounting for 4.1% of the total genome, were detected as single nucleotide polymorphisms or small indels, suggesting multiple introductions of this invaded species from its native area into the country. The high level of genetic diversity of the pine wood nematode was related to its pathogenicity and ecological trait differences. Moreover, we identified a gene set affected by genomic variation, and functional annotation of those genes indicated that some of them had potential roles in pathogenesis. Conclusions: This study provides an important resource for understanding the population structure, pathogenicity and evolutionary ecology of the nematode, and further analysis based on this study with geographically diverse B. xylophilus populations will greatly accelerate our understanding of the complex evolutionary/epidemic history of this emerging pathogen.
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Shimizu S, Kikuchi T, Koga M, Kato Y, Matsuoka H, Maruyama H, Kimura M, Research Group on Chemotherapy of Tropical Diseases.
Travel medicine and infectious disease 13 ( 3 ) 235 - 40 2015年5月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Travel Medicine and Infectious Disease
© 2014 Elsevier Ltd. All rights reserved. Background Recently, a dose of 30 mg (base) primaquine daily for 14 days is increasingly recommended for radical cure of Plasmodium vivax malaria. However, total primaquine doses, or those per body weight, are also recognized as important. In Japan, primaquine is not a licensed medicine, but has been used through the Research Group on Chemotherapy of Tropical Diseases for > 3 decades. Methods Based on clinical records submitted to the Research Group, patients with P. vivax and Plasmodium ovale malaria treated with primaquine were analyzed to determine the efficacy and safety of the antimalarial drug. Results Seventy-five P. vivax cases, including 3 in children, and 19 P. ovale cases were enrolled. Five of the P. vivax cases demonstrated at least one relapse despite primaquine therapy. Total primaquine doses per body weight were obtained in 4 of the 5 relapsed patients, presenting 9 malaria episodes totally, and most of the primaquine failures were caused with a total dose ≥3.5 mg/kg. Liver function disturbance was reported in 2 cases. Conclus ion In order to optimize radical cure of P. vivax, the total primaquine dose per body weight should be considered, at least 3.5 mg/kg or even more if contracted in countries with significant drug resistance. Possibility of primaquine hepatotoxicity in chronic liver disease patients remains to be elucidated.