論文 - 丸山 治彦
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Comparison of a 22-gauge Franseen-tip needle with a 20-gauge forward-bevel needle for the diagnosis of type 1 autoimmune pancreatitis: a prospective, randomized, controlled, multicenter study (COMPAS study).
Kurita A, Yasukawa S, Zen Y, Yoshimura K, Ogura T, Ozawa E, Okabe Y, Asada M, Nebiki H, Shigekawa M, Ikeura T, Eguchi T, Maruyama H, Ueki T, Itonaga M, Hashimoto S, Shiomi H, Minami R, Hoki N, Takenaka M, Itokawa Y, Uza N, Hashigo S, Yasuda H, Takada R, Kamada H, Kawamoto H, Kawakami H, Moriyama I, Fujita K, Matsumoto H, Hanada K, Takemura T, Yazumi S
Gastrointestinal endoscopy 91 ( 2 ) 373 - 381.e2 2020年2月
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寄生虫症抗体検査のこれまでとこれから
丸山治彦
Clinical Parasitology 2019年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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寄生虫症抗体検査のこれまでとこれから
丸山治彦
Clinical Parasitology 2019年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Establishment of a serodiagnosis system for the detection of Toxocara spp. and Ascaris suum infection in chickens. 査読あり 国際共著 国際誌
Nguyen YTH, Hayata Y, Sonoda S, Nonaka N, Maruyama H, Yoshida A.
Parasitology International 75 102022 - 102022 2019年11月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Parasitology International
Chickens are considered to act as paratenic hosts for agents, Toxocara canis, T. cati and Ascaris suum; which cause ascarid larva migrans syndrome (ascarid LMS) in humans. In addition, they are the definitive host for Ascaridia galli, considered not to be infective for humans. All ascarid parasites can have a high homology of antigenicity, leading to cross-reactivity in serodiagnostic assays. This study was conducted to establish a procedure for the serological detection of those roundworm infections in chickens. Twenty-five male Julia chickens were divided into five groups (n = 5); T. canis-, T. cati-, Ascaris suum- and Ascaridia galli-infected, and an uninfected control group. In Ascaris suum-soluble worm antigen preparation (As-SWAP) ELISA, all infected groups showed an elevation of anti-ascarid antibodies, indicating the usefulness of As-SWAP as a screening antigen for the detection of ascarid infections. For infecting species identification, T. canis-excretory/secretory (Tc-ES) and Ascaris suum-ES (As-ES) antigen ELISA were conducted by serial dilution sera. Toxocara spp.-infected sera showed stronger binding to Tc-ES than As-ES, while Ascaris suum and Ascaridia galli-infected sera bound to As-ES more strongly than Tc-ES. To discriminate between Ascaris suum and Ascaridia galli infection, sera were pre-incubated with Ascaridia galli-SWAP antigen and applied to Tc-ES and As-ES ELISAs. In this pre-adsorbed ES antigen ELISAs, only the Ascaris suum infected group showed positive binding to As-ES, resulting from the adsorption of cross-reactive antibodies in Ascaridia galli-infected sera. Finally, anti-Toxocara specific antibodies were confirmed by Tc-ES western blot (WB). Toxocara spp.-infected sera showed toxocariasis-specific band pattern in Tc-ES WB, while no specific band appeared on any strip incubated with Ascaris suum, Ascaridia galli-infected and uninfected sera. In conclusion, the serodiagnostic assays evaluated in this study are useful for the detection of ascarid infections in chickens.
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Characterization of the RAGE-binding protein, Strongyloides venestatin, produced by the silkworm-baculovirus expression system. 査読あり
Tsubokawa D, Lee JM, Hatta T, Mikami F, Maruyama H, Arakawa T, Kusakabe T, Tsuji N.
Infection, Genetics and Evolution 2019年11月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Paragonimus and paragonimiasis in Asia: An update. 査読あり
Yoshida A, Doanh PN, Maruyama H.
Acta Tropica 2019年11月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Improved 18S and 28S rDNA primer sets for NGS-based parasite detection. 査読あり
Kounosu A, Murase K, Yoshida A, Maruyama H, Kikuchi T.
Scientific Reports 2019年10月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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First molecular identification of Strongyloides vituli in cattle in Japan and insights into the evolutionary history of Strongyloides parasites of ruminants. 査読あり
Ko, P.P., Sakaguchi, K., Yoshida, A., Maruyama, H., Nonaka, N., Nagayasu E.
Parasitology International 2019年10月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Sequential Changes in the Host Gut Microbiota During Infection With the Intestinal Parasitic Nematode Strongyloides venezuelensis. 査読あり
Afrin T., Murase K., Kounosu A., Hunt V., Bligh M., Maeda Y., Hino A., Maruyama H., Tsai I., Kikuchi T.
Frontiers in Cellular and Infection Microbiology 2019年6月
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Maeda Y., Palomares-Rius J., Hino A., Afrin T., Mondal S., Nakatake A., Maruyama H., Kikuchi T.
Parasites and Vectors 12 ( 1 ) 21 2019年1月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Parasites and Vectors
© 2019 The Author(s). Background: Parasites excrete and secrete a wide range of molecules that act as the primary interface with their hosts and play critical roles in establishing parasitism during different stages of infection. Strongyloides venezuelensis is a gastrointestinal parasite of rats that is widely used as a laboratory model and is known to produce both soluble and insoluble (adhesive) secretions during its parasitic stages. However, little is known about the constituents of these secretions. Results: Using mass spectrometry, we identified 436 proteins from the infective third-stage larvae (iL3s) and 196 proteins from the parasitic females of S. venezuelensis. The proteins that were secreted by the iL3s were enriched with peptidase activity, embryo development and the oxidation-reduction process, while those of the parasitic females were associated with glycolysis, DNA binding (histones) and other unknown functions. Trypsin inhibitor-like domain-containing proteins were identified as the main component of the adhesive secretion from parasitic females. An absence of secretion signals in many of the proteins indicated that they are secreted via non-classical secretion pathways. Conclusions: We found that S. venezuelensis secretes a wide range of proteins to establish parasitism. This includes proteins that have previously been identified as being involved in parasitism in other helminths as well as proteins that are unique to this species. These findings provide insights into the molecular mechanisms underlying Strongyloides parasitism.
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Prevalence and associated risk factors of Strongyloides stercoralis infection in Lower Myanmar. 査読あり
Aung M., Hino A., Oo K., Win K., Maruyama H., Htike W., Nagayasu E.
Tropical medicine and Health 46 ( 1 ) 43 2018年12月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Tropical Medicine and Health
© 2018 The Author(s). Background: Strongyloidiasis is prevalent in Southeast Asian regions along with other soil-transmitted helminthiases, but only limited present-day data was available for Myanmar. Methods: A prevalence survey for Strongyloides stercoralis infection was conducted among villagers in rural areas of three townships located in the Lower Myanmar during 2014-2016 by agar plate culture method in combination with specific identification by molecular assays. Risk factors associated with S. stercoralis infection were assessed by analyzing questionnaires obtained from study participants. Results: Strongyloides stercoralis was identified in 40 out of 703 participants (5.7% overall prevalence). The highest prevalence (14.4%) was observed in Htantabin, while other two communities (Thabaung and Thanlyin) had much lower prevalence (2.2 and 2.5%, respectively). Infection was relatively rare (1.2%) in younger generations under 20 years compared to older generations (9.5%). Even in Htantabin, none of the female residents under age 40 (n = 33) had infection. In adult Htantabin residents, those who answered that they do not wear shoes regularly had an elevated risk of infection (odds ratio = 2.50, 95% confidence interval = 1.03-6.08). Conclusions: This study showed that there is still an on-going transmission of strongyloidiasis in Lower Myanmar. It is highly desirable that the soil should be free of fecal contamination by improving the management of fecal waste. Meanwhile, health education to promote shoe-wearing would be beneficial to reduce the risk of transmission, especially for those who have frequent and intense contact with soil.
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ヒトとイヌに由来するStrongyloides stercoralisの分子系統解析
長安英治, 丸山治彦
Clinical Parasitology 2018年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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ヒトとイヌに由来するStrongyloides stercoralisの分子系統解析
長安英治, 丸山治彦
Clinical Parasitology 2018年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Evaluation of LIPS (luciferase immunoprecipitation system) for serodiagnosis of Toxoplasmosis. 査読あり
Aye, K.M., Nagayasu, E., Baba, M., Yoshida, A., Takashima, Y., Maruyama, H.
Journal of immunological methods 462 91 - 100 2018年11月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Immunological Methods
© 2018 Elsevier B.V. Development of reliable, quantitative technologies for serodiagnosis of Toxoplasma gondii infection remains desirable. The luciferase immunoprecipitation system (LIPS) is a relatively simple, highly sensitive, and rapid quantitative immunoassay. The major advantages of this assay over ELISA are a wider dynamic range, shorter overall assay time, and less sample volume. In this study, we aimed to use this method for the serodiagnosis of toxoplasmosis. Recombinant Toxoplasma antigens (dense granule antigens GRA6, GRA7, and GRA8 and bradyzoite antigen BAG1) fused with nanoluciferase (Nluc, a small luciferase enzyme) were expressed in Escherichia coli, purified, and tested in LIPS assays with sera from experimental mice infected with T. gondii and a WHO standard anti-Toxoplasma human immunoglobulin (TOXM). In the experimentally infected mice, LIPS assays detected antibodies against Nluc-GRA6, Nluc-GRA7, and Nluc-GRA8 as early as day 14, whereas antibodies against Nluc-BAG1 remained undetected until day 21 and then showed significant elevation on day 60. In TOXM sera, LIPS assays with each Nluc recombinant protein produced reliable standard curves with a coefficient of determination (R 2 ) of 0.980–0.989 for GRA6, 0.986–0.990 for GRA7, 0.998–0.999 for GRA8, and 0.942–0.987 for BAG1. The detection limits were estimated to be 3.9, 2, 1, and 1 IU/ml for rGRA6, rGRA7, rGRA8, and rBAG1, respectively. The LIPS assay for toxoplasmosis could detect antibodies against T. gondii in the mouse and human sera with a reasonably high sensitivity. We consider the LIPS assay to be a promising alternative tool for screening, diagnosing, and monitoring toxoplasmosis. In particular, detection of antibodies against BAG1 may be useful for a longitudinal seroprevalence study in suspected high-risk areas on the basis of its elevated serum concentration in the chronic phase.
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Takotsubo cardiomyopathy associated with Paragonimiasis westermani. 査読あり
Matsuoka R, Muneuchi J, Nagatomo Y, Shimizu D, Okada S, Iida C, Shirouzu H, Watanabe M, Takahashi Y, Maruyama H
Paediatrics and international child health 38 ( 4 ) 302 - 307 2018年11月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Paediatrics and International Child Health
© 2017, © 2017 Informa UK Limited, trading as Taylor & Francis Group. An 11-year-old boy collapsed during morning assembly at his junior high school. The automated external defibrillator detected ventricular fibrillation and provided shock delivery. He was successfully resuscitated and reverted to sinus rhythm. Electrocardiography showed ST-T elevation in the precordial leads. Echocardiography and angiography demonstrated akinesia of the apex and mid-wall of the left ventricle with preserved contraction of the basal segments, which suggested Takotsubo cardiomyopathy. The patient and his family had often eaten uncooked crab, and his father had a past history of infection with Paragonimiasis westermani. The patient had had a persistent cough and chest pain for several weeks. Chest radiograph showed cystic cavities in the left upper lung. Microbiological examination of the sputum demonstrated an egg of P. westermani and immunological assay showed a raised antibody titre to P. westermani. On the12th day of admission, he developed seizures, and magnetic resonance imaging demonstrated cerebral involvement. After the administration of praziquantel for 3 days, the clinical manifestations improved immediately, and echocardiography normalised within 3 weeks. The patient was discharged on the 32nd day + and follow-up was normal. Takotsubo cardiomyopathy following a potentially fatal arrhythmia is a rare cardiac complication associated with pulmonary and central nervous system infection by P. westermani.
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Cerebral Paragonimiasis With Hemorrhagic Stroke in a Developed Country. 査読あり
Kashida Y, Niiro M, Maruyama H, Hanaya R
Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association 27 ( 10 ) 2648 - 2649 2018年10月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Stroke and Cerebrovascular Diseases
© 2018 Elsevier Ltd Paragonimiasis is a food-borne parasitic disease caused by Paragonimus lung flukes, which are epidemic in Asia. Cerebral paragonimiasis accounts for <1% of symptomatic paragonimiasis but is the most common extrapulmonary infection. Cerebral paragonimiasis often mimics stroke and sometimes causes severe neurological sequelae. A 61-year-old woman was admitted to the hospital for severe headache. A head computed tomography scan revealed intracerebral hemorrhage with subarachnoid hemorrhage. The patient also had lesions in the lungs. She frequently ate Japanese mitten crab. Peripheral blood examination results of increased eosinophilia and immunological testing results confirmed the diagnosis of Paragonimus westermani infection. The patient was successfully treated with praziquantel as the first-line agent. Cerebral paragonimiasis is currently rare in developed countries; however, it is an important disease to consider.
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Ohno T, Kai T, Miyasaka Y, Maruyama H, Ishih A, Kino H
Parasitology international 67 ( 4 ) 357 - 361 2018年8月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Parasitology International
© 2018 Elsevier B.V. Hymenolepis diminuta is a parasitic tapeworm of the rat small intestine and is recognized as a useful model for the analysis of cestode-host interactions. In this study, we analyzed factors affecting the biomass of the tapeworm through use of rat strains carrying genetic mutations, namely X-linked severe combined immunodeficiency (xscid; T, B and NK cells deficiency), nude (rnu; T cell deficiency), and mast cell deficient rats. The worm biomass of F344-xscid rats after infection with 5 cysticercoids was much larger than control F344 rats from 3 to 8 weeks. The biomass of F344-rnu rats was also larger than the controls, but was intermediate between F344-xscid and control rats. These observations demonstrated that host immunity can control the maximal tapeworm biomass, i.e., carrying capacity, of the rat small intestine. Both T cell and other immune cells (B and NK cells) have roles in determining the carrying capacity of tapeworms. Total worm biomass and worm numbers in mast cell deficient rats (WsRC-Ws/Ws) were not significantly different from control WsRC-+/+ rats after 3 and 6 weeks of primary infection. Mast cell deficient rats displayed reinfection resistance for worm biomass but not worm expulsion. These findings suggest that the mast cell has a role for controlling the biomass of this tapeworm in reinfection alone, but does not affect the rate of worm expulsion. Overall, our findings indicate that the mast cell is not a major effector cell for the control of the carrying capacity of tapeworms. The identity of the major effector cell remains unknown.
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Wang, Z., Shibata, M., Nguyen, Y.T.H., Hayata, Y., Nonaka, N., Maruyama, H., Yoshida, A.
Parasitology international 67 ( 5 ) 622 - 626 2018年6月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Parasitology International
© 2018 Elsevier B.V. Ascarid Larva Migrans Syndrome (ascarid LMS) is a clinical syndrome in humans, caused by the migration of animal roundworm larvae such as Toxocara canis, Toxocara cati and Ascaris suum. Humans may acquire infection by ingesting embryonated eggs, or infective larvae of these parasites in contaminated meat and organ meats. To detect these pathogenic contaminations, a novel nested multiplex PCR system was developed. Our novel nested multiplex PCR assay showed specific amplification of T. canis, T. cati and Ascaris spp. Detection limit of the nested multiplex PCR was tested with serial dilution of T. canis, T. cati or A. suum genomic DNA (gDNA) from 100 pg to 100 ag and found to be 10 fg, 1 fg and 100 fg, respectively. When larvae were spiked into chicken liver tissue, DNA of T. canis and A. suum was detected from the liver spiked with a single larva, while the assay required at least 2 larvae of T. cati. Moreover, the ascarid DNA was detected from the liver of mice infected with 100 and 300 eggs of T. canis, T. cati or A. suum. This nested multiplex PCR assay could be useful for the detection of contamination with ascarid larvae in meat and organ meats.
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Nakamura-Uchiyama F, Katanami Y, Kikuchi T, Takaya S, Kutsuna S, Kobayashi T, Mizuno Y, Hasegawa T, Koga M, Yoshimura Y, Hasegawa C, Kato Y, Kimura M, Maruyama H, Research Group on Chemotherapy of Tropical Diseases, Japan.
Travel medicine and infectious disease 22 40 - 45 2018年3月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Travel Medicine and Infectious Disease
© 2018 Elsevier Ltd Background: The Research Group on Chemotherapy of Tropical Diseases, Japan, introduced artemether–lumefantrine (AL) in late 2002, mainly for treating uncomplicated Plasmodium falciparum malaria. Because AL was on the market in Japan in March 2017, the effectiveness and safety of AL were analyzed to help medical personnel use AL optimally. Methods: Case report forms submitted by the attending physicians were analyzed. When necessary, direct contact with the attending physicians was made to obtain detailed information. Results: Effectiveness analysis was performed for 62 cases and safety analysis was performed for 66 cases. In P. falciparum malaria, the overall cure rate was 91.1% (51/56), of which the cure rates for Japanese and non-Japanese patients were 82.1% (23/28) and 100% (28/28), respectively. The successfully treated cases included severe P. falciparum malaria, with parasite densities exceeding 500,000/μL. Adverse events were reported in 14 patients, including delayed hemolytic anemia which occurred in the top four highest parasitemic cases. Conclusions: AL treatment failure in P. falciparum malaria may not be rare among non-immune individuals, including Japanese. The possibility of delayed hemolytic anemia, which occurs preferentially in high parasitemic cases, should be considered following AL treatment.
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Hasegawa C, Kudo M, Maruyama H, Kimura M
Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy 24 ( 3 ) 216 - 219 2018年3月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Infection and Chemotherapy
© 2017 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases Delayed haemolytic anaemia has been reported in association with intravenous artesunate treatment in patients with severe Plasmodium falciparum malaria, and furthermore, oral artemisinin-based combination therapies including artemether-lumefantrine (AL) have also been incriminated. However, definite cases of delayed haemolytic anaemia associated with AL appear to be scarce, as reported cases were often treated concomitantly with other anti-malarials. In this study, we report a severe case of delayed haemolytic anaemia following AL alone in a Japanese traveller with severe parasitaemia caused by numerous P. falciparum parasites and a few P. vivax parasites. We also stress the need by further studies to differentiate between delayed haemolytic anaemia and blackwater fever, the latter being another malaria-related haemolytic condition, more clearly than they are now.