論文 - 河原 聡
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Nakanishi T., Uchiyama T., Uchida M., Erickson L., Kawahara S.
British Poultry Science 2024年10月
担当区分：最終著者 掲載種別：研究論文（学術雑誌） 出版者・発行元：British Poultry Science
1. This study investigated the role of glutathione peroxidase 4 (GPX4), a key regulator of ferroptosis, a form of programmed cell death, in muscle biochemistry and meat quality, utilising broiler chickens whose ante-mortem GPX4 activity was inhibited pharmacologically. 2. Male broilers were divided into two groups, each receiving ante-mortem administration of the GPX4 inhibitor, Ras-selective lethal 3 (RSL3), or a vehicle only. After slaughter, breast muscles were collected and stored for 48 h. The expressions of ferroptosis-related genes, glutathione levels, pH, colour and water-holding capacity were evaluated at multiple time points during the storage period. 3. The RSL3 treatment decreased the expression of GPX4 and ferritin heavy chain 1, which are negative regulators of ferroptosis, while it increased the expression of a ferroptosis accelerator, acyl-CoA synthetase long chain family member 4. The ratio of reduced to oxidised glutathione was significantly decreased in the RSL3 group. The RSL3 treatment decelerated post-mortem pH decline and colour changes, such as a decrease in L* and an increase in a* were observed in the RSL3 group. In addition, the RSL3 group showed increased levels of water-holding capacity. 4. These findings suggested that ante-mortem GPX4 activity plays a role in determining meat quality, implying the possible involvement of ferroptosis in the mechanism by which skeletal muscle is converted after slaughter into meat that is eaten.
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カンショデンプン粕を給与した肥育豚の発育成績と肉質 査読あり
川島 知之, 林田 良, 河原 聡, 小林 郁雄, 高橋 俊浩
日本暖地畜産学会報 67 ( 2 ) 101 - 109 2024年9月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：日本暖地畜産学会
カンショデンプン粕を給与した肥育豚の発育成績と肉質を調査した．LWD 交雑種の去勢雄豚を対照区と試験区に4 頭ずつ配置した．試験開始体重は平均70 ㎏で，単独飼育，飽食給与，自由飲水で肥育試験を行い，体重約110 ㎏で出荷した．両区ともに可消化養分総量，粗タンパク質および細胞壁成分（OCW）が同じになるように，トウモロコシおよび大豆粕を主体として，対照区ではフスマを，試験区ではカンショデンプン粕を配合する設計とした．試験区の飼料は，OCW 中のセルラーゼ可溶画分（Oa）が対照区より多くなった．試験区では対照区と比較して乾物飼料摂取量，日増体量が低くなった．また，豚肉の理化学特性に有意差はなかったが，皮下脂肪内層中のオレイン酸含量が試験区で増加する傾向が見られた．試験区で糞中窒素含量が有意に増加した．官能評価において，試験区のカンショデンプン粕を給与した豚肉は香りの点で好ましいとの評価が高く，総合評価も高かった．<i>日本暖地畜産学会報67（2）：101-109，2024</i>
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Nakanishi T., Suga K., Wakitani S., Yamaguchi K., Sugamoto K., Erickson L., Kawahara S.
European Journal of Lipid Science and Technology 2024年3月
担当区分：最終著者 記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：European Journal of Lipid Science and Technology
Phytanic acid is one of the branched-chain fatty acids in the human diet. Humans incorporate it into their bodies through the ingestion of ruminant meat and milk since these animal products contain the 3RS, 7R, 11R-isomer, as naturally occurring phytanic acid (nPA). Although phytanic acid has been reported to have health-promoting effects such as immunomodulatory effects, these findings were obtained mostly from in vitro cell-based experiments. In this study, the in vivo anti-inflammatory effects of nPA were investigated using a mouse model of dextran sulfate sodium (DSS)-induced colitis. Mice were fed diets containing 0%, 0.05%, or 0.1% nPA for 4 weeks. During the fourth week, acute colitis was induced by administration of a 3% DSS solution. The intake of 0.05% and 0.1% nPA inhibited DSS-induced body weight loss in mice, and showed improvement of histopathological changes in colon epithelium. nPA also inhibited the production of proinflammatory cytokines, especially macrophage-related cytokines such as interleukin-1β in the colon. These results suggest that nPA exerts anti-inflammatory effects under in vivo physiological conditions. Practical application: There is a growing demand for fatty acids possessing distinct nutritional and physical characteristics in the realms of health, nutrition, and food-related applications. The present study demonstrated that a branched-chain fatty acid, phytanic acid, exerts anti-inflammatory activity under not only in vitro but also in vivo conditions, using a commonly used mouse model of human inflammatory bowel disease. Our findings encourage further studies to determine whether phytanic acid elicits beneficial properties in humans, and suggest that phytanic acid enrichment can be a novel product strategy in the food market.
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In vitro characterization of anti-inflammatory activities of 3RS, 11R-phytanic acid 査読あり 国際共著
Tomonori Nakanishi, Mikihisa Izumi, Ryoji Suzuki, Kohta Yamaguchi, Kazuhiro Kagamizono, Laurie Erickson, Satoshi Kawahara
Journal of Dairy Research 2023年3月
担当区分：最終著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
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In vitro characterization of anti-inflammatory activities of 3RS, 7R, 11R-phytanic acid
Nakanishi T., Izumi M., Suzuki R., Yamaguchi K., Sugamoto K., Erickson L., Kawahara S.
Journal of Dairy Research 90 ( 1 ) 92 - 99 2023年2月
掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Dairy Research
The aim of the research described here was to investigate the in vitro immunomodulatory effects of 3RS, 7R, 11R-phytanic acid (3RS-PHY) from the perspective of efficacy against autoimmune diseases. 3RS-PHY is a milk component with strong agonist activity at the peroxisome proliferator activated receptor (PPAR). As PPAR is a therapeutic target for several human diseases, 3RS-PHY intake may have possible health benefits. Recently, we chemically synthesized a preparation of 3RS-PHY and demonstrated that 3RS-PHY inhibited T-cell production of interferon (IFN)-γ. However, the overall immunomodulatory effects were not evaluated. In this study, mouse splenocytes, purified T-cells and B-cells were stimulated by mitogens and incubated with 3RS-PHY, followed by evaluation of cytokine and antibody production. A macrophage-like cell line J774.1 was also incubated with 3RS-PHY to evaluate nitric oxide production. 3RS-PHY decreased mRNA levels not only of IFN-γ but also of interleukin (IL)-2, IL-10 and IL-17A in splenocytes and similar effects were confirmed at the protein level. In addition, 3RS-PHY had a direct action on T-cells with preferential inhibitory effects on Th1 and Th17 cytokines such as IFN-γ and IL-17A. Furthermore, 3RS-PHY suppressed antibody secretion by B-cells and nitric oxide production by J774.1 almost completely, indicating that 3RS-PHY is a bioactive fatty acid with anti-inflammatory properties. These findings encourage further investigations, including in vivo experiments, to evaluate whether 3RS-PHY actually shows the potential to prevent autoimmune diseases, and provide basic information to produce milk and dairy products with an increased 3RS-PHY concentration.
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Antemortem inhibition of phosphoinositide 3-kinase causes changes in meat quality traits in broiler chickens 査読あり 国際共著
Nakanishi T, Watanabe K, Erickson L, Kawahara S
Domestic Animal Endocrinology 75 1 - 7 2021年4月
担当区分：責任著者 記述言語：英語 掲載種別：研究論文（学術雑誌）
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Dietary phytanic acid-induced changes in tissue fatty acid profiles in mice 査読あり
Tomonori Nakanishi, Kazuhiro Kagamizono, Sayaka Yokoyama, Ryoji Suzuki, Hiroyuki Sakakibara, Kazuhiro Sugamoto, Laurie Erickson, Satoshi Kawahara
Journal of Dairy Research 87 ( 4 ) 498 - 500 2020年11月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Tomonori Nakanishi, Kazuhiro Kagamizono, Sayaka Yokoyama, Ryoji Suzuki, Hiroyuki Sakakibara, Laurie Erickson, Satoshi Kawahara
Animal Science Journal 91 ( 1 ) 1 - 8 2020年7月
記述言語：英語 掲載種別：研究論文（学術雑誌）
DOI： 10.1111/asj.13424
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Changes in expression of the autophagy-related genes microtubule-associated protein 1 light chain 3ß and autophagy related 7 in skeletal muscle of fattening Japanese Black cattle: a pilot study 査読あり
Nakanishi T., Tokunaga T., Ishida T., Kobayashi I., Katahama Y., Yano A., Erickson L., Kawahara S.
Asian-Australasian Journal of Animal Science 32 ( 4 ) 592 - 598 2019年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
DOI： 10.5713/ajas.18.0370
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Naturally occurring 3RS,7R,11R-phytanic acid suppresses in vitro T-cell production fo interferon-gamma 査読あり
Nakanishi T., Motoba I., Anraku M., Suzuki R., Yamaguchi Y., Erickson L., Eto N., Sugamoto K., Matsushita Y., Kawahara S.
Lipids in Health and Disease 17 147 - 154 2018年6月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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カンショ由来生焼酎粕給与が肥育豚の飼養成績と肉質に及ぼす影響 査読あり
勝俣 沙智, 鎌倉 美彩子, 磯島 聖良, 高橋 俊浩, 小林 郁雄, 河原 聡, 坂本 信介, 新美 光弘, 川島 知之
日本畜産学会報 89 ( 2 ) 199 - 206 2018年6月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
焼酎の蒸留副産物である生焼酎粕は揮発性のアルコールや有機酸を含むが，熱乾燥法により求めた成分値にはこれらが反映されていない．生焼酎粕を飼料原料として有効活用するため，生焼酎粕を含む飼料を肥育豚に給与し，飼養成績と肉質に及ぼす影響を調べた．LWD去勢雄8頭（体重69kg）を，4頭ずつ生焼酎粕区と乾燥焼酎粕区に割り当てた．両区ともに乾物あたり焼酎粕17%，配合飼料73%，トウモロコシ10%の割合で配合した飼料を給与し体重約115kgまで肥育した．行動解析により生焼酎粕を給与した肥育豚は活動性が低く，より多く休息していることが分かった．増体日量に差はなく，生焼酎粕供与による飼育成績は良好だった．乾燥焼酎粕区と比較し生焼酎粕区の胸最長筋のL*値が低い傾向にあった．また，胸最長筋の剪断力価は低い傾向にあり，消費者型官能評価によっても生焼酎粕給与により肥育された豚肉は柔らかいという結果となった．
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Effect of pre-slaughter autophagic status on postmortem proteolysis in skeletal muscle of mice 査読あり
Nakanishi, T., Yamashita, M., Nishimoto, N., Erickson, L., Kawahara, S.
American Journal of Animal and Veterinary Sciences 12 ( 2 ) 58 - 64 2017年12月
記述言語：英語 掲載種別：研究論文（学術雑誌） 出版者・発行元：American Journal of Animal and Veterinary Sciences
© 2017 Tomonori Nakanishi, Miho Yamashita, Naoto Nishimoto, Laurie Erickson and Satoshi Kawahara. Post mortem proteolysis is a key event during the aging process, where muscle converts to meat. However, its precise mechanism remains unclear. In this study, the effects of pre-slaughter autophagic status on post mortem proteolysis were investigated using mouse skeletal muscle. Mice were fasted for 14 h and administered with or without the autophagy inhibitor wortmannin, whereas control mice were given free access to diet. Skeletal muscle samples were collected and stored at 4°C. Muscle proteins were extracted at several time points, followed by evaluation of microtubule-associated protein Light Chain 3 (LC3)-II expression as an autophagic marker and of post mortem changes in water-soluble proteins by High Performance Liquid Chromatography (HPLC) analysis. Mice fasted before sacrifice showed significantly higher expression of LC3-II than control mice and this effect was inhibited by the administration of wortmannin. Results on HPLC analysis indicated typical post mortem changes in water-soluble muscle proteins and these changes were accelerated when autophagy was induced by starvation. In addition, the administration of wortmannin canceled acceleration of post mortem changes by starvation. These findings could suggest that autophagy is a mediator of post mortem proteolysis in skeletal muscle and contributes to meat quality.
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Investigation of lactic acid bacterial strains for meat fermentation and the product's antioxidant and angiotensin-I-converting enzyme inhibitory activities 査読あり
Takeda, T., Matsufuji, H., Nakade, K., Takenoyama, S., Ahhmed, A., Sakata, R., Kawahara, S., Muguruma, M.
Animal Science Journal 88 507 - 516 2017年3月
記述言語：英語 掲載種別：研究論文（学術雑誌）
DOI： 10.1111/asj.12673
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Orally administered conjugated linoleic acid ameliorates allergic dermatitis induced by repeated applications of oxazolone in mice 査読あり
Nakanishi, T., Tokunaga, Y., Yamasaki, M., Erickson, L., Kawahara, S.
Animal Science Journal 87 1554 - 1561 2016年12月
記述言語：英語 掲載種別：研究論文（学術雑誌）
DOI： 10.1111/asj.12603
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ACE inhibitory activity of original peptides derived from "milky miso" on hypertensive rats and people 査読あり
Muguruma, M., Kawahara, S., Kusumegi, K., Hishinuma, T., Maruyama, M., Nakamura, T.
Proceedings of the 17th AAAP Animal Science Congress 17 287 - 287 2016年8月
記述言語：英語 掲載種別：研究論文（国際会議プロシーディングス）
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Protein degradation by autophagy during postmortem storage of mouse skeletal muscle 査読あり
Yamashita, M., Kawahara, S., Morita, T., Kaminaka, S., Sakakibara, S., Nakanishi, T.
Proceedings of the 17th AAAP Animal Science Congress 17 287 - 287 2016年8月
記述言語：英語 掲載種別：研究論文（国際会議プロシーディングス）
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Phytanic acid and pristanic acid contents in milk and dairy products originated from Japan and European countries 査読あり
Suzuki, R., Nakanishi, T., Matsushita, Y., Kawahara, S.
Proceedings of the 17th AAAP Animal Science Congress 17 361 - 361 2016年8月
記述言語：英語 掲載種別：研究論文（国際会議プロシーディングス）
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Novel immunomodulatory effects of phytanic acid and its related substances in mice 査読あり
Tomonori Nakanishi, Mayuko Anraku, Ryoji Suzuki, Tomoya Kono, Laurie Erickson, Satoshi Kawahara
Journal of Functional Foods 21 283 - 289 2016年2月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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各種食肉の消化酵素分解物における抗酸化作用の検討－その畜種別差異の比較検討－ 査読あり
竹田 志郎, 久保田 大樹, 竹之山 愼一, 河原 聡, 六車 三治男
日本暖地畜産学会報 58 ( 1 ) 103 - 108 2015年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Warm Regional Society of Animal Science, Japan
本研究では食肉の消化酵素分解物の抗酸化作用についてDPPH ラジカル消去法および酸素ラジカル吸収能(ORAC) 法により検討した。その結果、牛肉、豚肉、鶏肉の消化酵素分解物に、抗酸化物質であるカルノシンと同等以上の抗酸化活性が認められたが、それらの活性値に大きな差異は認められなかった。ORAC 法により、各種食肉の消化酵素分解物の抗酸化力持続能を比較したところ、消化酵素分解鶏肉の抗酸化力持続継時変化様式は、消化酵素分解牛肉および豚肉の継時変化様式と異なった。この要因として、消化酵素処理により生じたペプチドの種類が影響している可能性が考えられた。以上より、ヒトが食肉を摂取したとき、体内で消化により生じる食肉分解物が抗酸化作用を発揮する可能性が考えられた。また、各種食肉消化酵素分解物を構成するアミノ酸類の違いにより、 ORAC 法の抗酸化力持続継時変化様式が異なることが示唆された。
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Antiallergic activity of probiotics from Mongolian dairy products on type I allergy in mice and mode of antiallergic action 査読あり
Takeda S., Hidaka M., Yoshida H., Takeshita M., Kikuchi Y., Tsend-Ayush C., Dashnyam B., Kawahara S., Muguruma M., Watanabe W., Kurokawa M.
Journal of Functional Foods 9 ( 4 ) 60 - 69 2014年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）