論文 - 河原 聡
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低分子ヘパリン中の夾雑ムコ多糖類の工業的除去方法 査読あり
村田浩志，嶋村英雄，松久明生，山本啓一，杉山幸司，川村隆生，余田 光，植村英俊，戸田隆雄，六車三治男，河原 聡
日本食品科学工学会誌 61 ( 2 ) 85 - 94 2014年2月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
DOI： 10.3136/nskkk.61.85
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Takeda S., Fujimoto R., Takenoyama S., Takeshita M., Kikuchi Y., Tsend-Ayush C., Dashnyam B., Muguruma M., Kawahara S.
Food Science and Technology Research 19 ( 2 ) 245 - 253 2013年7月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Food Science and Technology Research
We screened suitable lactic acid bacteria (LAB) strains for use in fermented dairy products by analyzing milk fermented with 10 probiotic LAB strains from Mongolian dairy products. Lactobacillus paracasei paracasei strain 06TCa19 was selected because of its favorable effects on pH, lactic acid production, and viable bacterial numbers after fermentation of skim milk. Then, we prepared 06TCa19 and control fermented milks and conducted a randomized, double-blind crossover study with 46 healthy women to determine the effects of the strain on human defecation. The ingestion of 06TCa19 fermented milk improved the subjects' fecal characteristics, including shape and color. Analysis of stool samples from 8 subjects revealed that l-lactic acid levels and Lactobacillus and Bifidobacterium numbers increased. Moreover, strain 06TCa19 was suggested to reach and survive in the intestines, and is, therefore, suitable for fermented dairy products and can potentially improve human defecation.
DOI： 10.3136/fstr.19.245
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Effects of oral administration of probiotics from Mongolian dairy products on the Th1 immune response in mice 査読あり
Takeda S., Kawahara S., Hidake M., Yoshida H., Watanabe W., Takeshita M., Kikuchi Y., Bumbein D., Muguruma M., Kurokawa M.
Bioscience, Biotechnology, and Biochemistry 77 ( 7 ) 1372 - 1378 2013年7月
記述言語：英語 掲載種別：研究論文（学術雑誌）
DOI： 10.1271/bbb.120624
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Cytokine mediated immune responses in the Japanese pufferfish (Takifugu rubripes) administered with heat-killed Lactobacillus paracasei spp. paracasei (06TCa22) isolated from the Mongolian dairy product 査読あり
Biswas G., Korenaga H., Nagamine R., Kawahara S., Takeda S., Kikuchi Y., Dashnyam B., Yoshida T., Kono T, Sakai M.
International Immunopharmacology 17 ( 2 ) 358 - 365 2013年7月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Elevated cytokine responses to Vibrio harveyi infection in the Japanese pufferfish (Takifugu rubripes) treated with Lactobacillus paracasei spp. paracasei (06CTa22) isolated from the Mongolian dairy products 査読あり
Biswas G., Korenaga H., Nagamine R., Kawahara S., Takeda S., Kikuchi Y., Dashnyam B., Yoshida T., Kono T, Sakai M.
Fish & Shellfish immunology 35 ( 3 ) 756 - 765 2013年6月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Cytokine responses in the Japanese pufferfish (Takifugu rubripes) head kidney cells induced with heat-killed probiotics isolated from the Mongolian dairy products 査読あり
Biswas G., Korenaga H., Nagamine R., Takayama H., Kawahara S., Takeda S., Kikuchi Y., Dashnyam B., Kono T, Sakai M.
Fish & Shellfish immunology 34 ( 5 ) 1170 - 1177 2013年5月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Application of probiotics from Mongolian dairy products to fermented dairy products and its effects on human defecation 査読あり
Takeda S., Fujimoto R., Takenoyama S., Takeshita M., Kikuchi Y., Tsend-Ayush C., Dashnyam B., Muguruma M., Kawahara S.
Food Science and Technology Research 19 ( 2 ) 245 - 253 2013年3月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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メタボリックシンドロームモデルラット（SHR/NDmcr-cp）における肝臓水解物の血糖に及ぼす影響 査読あり
井上尚典，日髙修二，三浦直良，山田耕太郎，深堀勝博，丸山眞杉，河原 聡，太田一良，六車三治男
薬学雑誌 133 ( 1 ) 117 - 123 2013年1月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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ブタ肝臓水解物の成分分析と抗酸化活性及びアンギオテンシン変換酵素（ACE）阻害活性の検討 査読あり
井上尚典，浜崎敦子，日髙修二，三浦直良，深堀勝博，丸山眞杉，河原 聡，太田一良，六車三治男
薬学雑誌 133 ( 1 ) 107 - 115 2013年1月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Inoue N., Inoue N., Hamasaki A., Hidaka S., Miura N., Fukahori M., Maruyama M., Kawahara S., Kawahara S., Ohta K., Ohta K., Muguruma M., Muguruma M.
Yakugaku Zasshi 133 ( 1 ) 107 - 115 2013年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Yakugaku Zasshi
Hypertension and oxidant stress predispose to the onset and progression of arteriosclerotic diseases. In this study, the components of two kinds of porcine liver hydrolysates (LH-I and LH-II) were analyzed, and the antioxidant effects and angiotensin converting enzyme (ACE)-inhibiting effects of LH-I and LH-II were examined in vitro. Furthermore, the effects of LH-I and LH-II on the blood pressure were examined in spontaneously hypertensive rats (SHR). The results showed that peptides and amino acids accounted for 70% or more of the constituents of both LH-I and LH-II. The results of gel filtration HPLC showed that most of the nitrogen-containing components were peptides or amino acids with molecular weights of 6000 or less. The DPPH radical scavenging activities of LH-I and LH-II were 55.6 and 38.1 mM Trolox Equivalent/g, respectively. The IC50 values for the ACE-inhibiting activity of LH-I and LH-II were 0.18 and 0.31 mg/mL, respectively. Oral administration of 1 g/rat of LH-I or LH-II to SHR resulted in significant lowering of the blood pressure. These findings indicate that both LH-I and LH-II have antioxidant activity and ACE-inhibiting activity. Moreover, both exerted a blood pressure-lowering effect in SHR. The antioxidant activity and ACE-inhibiting activity of LH-I and LH-II are presumed to be based on the actions of the component peptides. © 2013 The Pharmaceutical Society of Japan.
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Effects of liver hydrolysate on the blood glucose in metabolic syndrome model rats (SHR/NDmcr-cp)
Inoue N., Inoue N., Hidaka S., Miura N., Yamada K., Fukahori M., Maruyama M., Kawahara S., Kawahara S., Ohta K., Ohta K., Muguruma M., Muguruma M.
Yakugaku Zasshi 133 ( 1 ) 117 - 123 2013年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Yakugaku Zasshi
Insulin resistance associated with visceral fat obesity has been suggested to be the pathological basis of metabolic syndrome. Many studies have demonstrated increased oxidant stress in diabetic patients and animal models of diabetes mellitus. In this study, the effect of liver hydrolysate administration on the blood glucose was examined in SHR/NDmcrcp (SHR-cp) rats that show spontaneously occurring metabolic syndrome-like abnormalities. The SHR-cp rats were fed diets containing 5% liver hydrolysate for 12 weeks, and the fasting blood glucose and HbA1c were determined every 3 weeks. After administration of the liver hydrolysate-containing feed for 12 weeks, an oral glucose tolerance test was conducted and the plasma angiotensin II (AngII) concentrations were determined. The liver hydrolysate administration had no effect on the blood insulin levels in the oral glucose tolerance test, but significantly inhibited the d-glucose-induced increases of the blood glucose levels. Furthermore, the liver hydrolysate had almost no effect on the fasting blood glucose level, but tended to inhibit the increase of HbA1c. The plasma AngII concentration after the 12-week administration of liver hydrolysate remained significantly lower than that in the control group. These results indicate that a component of liver hydrolysate inhibits d-glucose-induced increase of the blood glucose level, and may improve insulin resistance. The angiotensin converting enzyme (ACE)-inhibiting effect and antioxidant effect of liver hydrolysate may be involved in this effect. © 2013 The Pharmaceutical Society of Japan.
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未分画ヘパリン中のムコ多糖類の確認方法およびその工業的除去方法 査読あり
村田浩志，松久明生，山本啓一，戸田隆雄，六車三治男，河原 聡
日本食品科学工学会誌 59 ( 12 ) 628 - 636 2012年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Murata H., Murata H., Matsuhisa A., Yamamoto K., Toda T., Muguruma M., Kawahara S.
Nippon Shokuhin Kagaku Kogaku Kaishi 59 ( 12 ) 628 - 636 2012年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Nippon Shokuhin Kagaku Kogaku Kaishi
We have developed an analytical method for mucopolysaccharides (MSs) and a simple method for removing their impurities from un-fractionated heparin (UFH) preparations. An over-sulfated chondroitin sulfate standard (OSCS-STD) was de-polymerized with nitrous acid, as were UFH sodium salt containing OSCS and other MSs (OSHP-SH), OSCS reference standards containing heparin (CSMS-CE1 and CSMS-CE2), and various UFH products. Following de-polymerization, the molecular weight distributions of these samples were analyzed by gel permeation HPLC. The molecular weight distribution of OSCS-STD remained unchanged before and after nitrous acid depolymerization, and the molecular weight distribution of OSCS-STD was similar to that of OSHP-SH de-polymerized with nitrous acid. On the other hand, the molecular weight distributions of CSMS-CE1 and CSMS-CE2 were similar to that of standard chondroitin sulfate B. UFH sodium salt and several drug substances containing heparin and other MSs were then purified by ethanol precipitation. After nitrous acid de-polymerization, gel permeation HPLC and 1HNMR spectroscopy revealed that MSs resistant to de-polymerization with nitrous acid were concentrated in the supernatant and heparin was concentrated in the colloidal precipitate.
DOI： 10.3136/nskkk.59.628
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A combination of soybean and skimmed milk reduces osteoporosis in rats 査読あり
Muguruma M., Ahhmed A.M., Kawahara S., Kusumegi K., Hishinuma T., Ohya K., Nakamura T.
Journal of Functional Foods 4 ( 4 ) 810 - 818 2012年10月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Efficacy of oral administration of heat-killed probiotics from Mongolian dairy products against influenza infection in mice: Alleviation of influenza infection by its immunomodulatory activity through intestinal immunity 査読あり
Takeda S., Takeshita M., Kikuchi Y., Dashnyam B., Kawahara S., Yoshida H., Watanabe W., Muguruma M., Kurokawa M.
International Immunopharmacology 11 ( 12 ) 1976 - 1983 2011年12月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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TAKEDA Shiro, YAMASAKI Keiko, TAKESHITA Masahiko, KIKUCHI Yukiharu, TSEND-AYUSH Chuluunbat, DASHNYAM Bumbein, AHHMED Abdulatef M., KAWAHARA Satoshi, MUGURUMA Michio
Animal science journal 82 ( 4 ) 571 - 579 2011年8月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Expression and postmortem proteolysis of cytoskeletal protein talin in chicken meat 査読あり
Yoshito Tomisaka, Abdulatef M. Ahhmed, Satoshi Kawahara, Michio Muguruma
Journal of Warm Regional Society of Animal Science, Japan 54 ( 1 ) 39 - 48 2011年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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冨阪 吉登, AHHMED Abdulatef M, 河原 聡, 六車 三治男
日本暖地畜産学会報 54 ( 1 ) 39 - 48 2011年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Warm Regional Society of Animal Science, Japan
細胞骨格タンパク質talinは，integrinとactin細胞骨格との間の重要な架橋として働いている．ある研究では，熟成した肉の保水性に関与すると報告されている．しかし，talinには２つのアイソフォーム，talin1（T1）およびtalin2（T2）があり，それぞれの生物学的役割は十分には理解されていない．また，T1とT2の肉質における特異的な役割も明確にはなっていない．本研究において，我々は，ニワトリの胸肉，腿肉，砂のう筋および心筋におけるT1とT2の発現量を調べた．また，腿肉におけるtalinの死後変化も調べた．T1のmRNA発現量は，砂のう筋で高く，胸肉で低かった（<I>P</I> < 0.01）が，T2の発現量は砂のう筋で低く胸肉で高かった（<I>P</I> < 0.01）．また，砂のう筋におけるT1は腿肉や心筋よりも高かった（<I>P</I> < 0.01）．8d4，anti-talin2または TA205抗体を用いてウエスタンブロットを行った結果，anti-talin2だけが骨格筋におけるtalinを検出することができた．また，免疫組織染色法においても，8d4ではなくanti-talin2が腿肉の筋繊維の周囲にtalinが局在することを示した．さらに，anti-talin2によってラベルされた腿肉のtalinは死後の貯蔵の間，早期に消失した．これらの結果から，ニワトリ骨格筋における（T1ではなく）T2の死後分解は，鶏肉の肉質に影響する可能性があると考えられた．
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Effects of osmotic dehydration sheets on freshness parameters of Thunnus thynnus stored at cold temperatures 査読あり
K. Saito, N. Hamada-Sato, A.M. Ahhmed, S. Kawahara, M. Muguruma
Journal of Food Processing and Preservation 34 1103 - 1120 2010年12月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Saito K., Hamada-Sato N., Ahhmed A., Ahhmed A., Kawahara S., Muguruma M., Muguruma M.
Journal of Food Processing and Preservation 34 ( 6 ) 1103 - 1120 2010年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Food Processing and Preservation
In this study, we evaluated the effects of osmotic dehydration sheets (ODSs) on quality parameters and morphological observation of tuna stored at 4C for 4 days. The use of ODSs did not affect Hunter L*and b*values, although use of low performance ODSs (ODS-L) gave higher a*values and lower metmyoglobin percentage (Met-Mb%) than other samples (P < 0.05). As increasingly higher-performance ODSs were used, the total sample content of ATP-related compounds and IMP (a component of umami) increased and the K values decreased (P < 0.05), indicating maintenance of tuna freshness. However, Hunter color values and Met-Mb% revealed that the optimum dehydration level exists at low dehydration performance. ODS was effective in removing intercellular water of thawed tuna tissue. We therefore suggest ODS-L as a functional tool for maintaining the color of tuna, and high performance ODS as a functional tool for maintaining freshness and reform watery texture of chilled stored tuna. © 2009 Wiley Periodicals, Inc.