論文 - 河原 聡
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Malonaldehyde content in some breads and its chage during storage. 査読あり
T. Sakai, S. Kawahara
J. Food Hyg. Soc. Japan 46 ( 3 ) 121 - 123 2005年3月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Inhibitory profile of nonapeptide derived from porcine troponin C against angiotensinⅠ-converting enzyme. 査読あり
K. Katayama, M. Tomatsu, S. Kawahara, K. Yamauchi, H. Fuchu, Y. Kodama, Y. Kawamura, M. Muguruma
J. Agric. Food Chem. 52 ( 4 ) 771 - 775 2004年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Sakai T., Munasinghe D., Kashimura M., Sugamoto K., Kawahara S.
Meat Science 66 ( 4 ) 789 - 792 2004年4月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Meat Science
Pork and beef containing NaCl were stored at 0 °C and changes in 4-hydroxynonenal (HNE) content were analyzed after 0, 3, 7 and 10 days. As an index of the lipid peroxidation level, malonaldehyde (MA) content was also analyzed. In pork, HNE content in samples with 2% NaCl increased (P<0.05) after 7 and 10 days of storage. In beef, HNE content in all samples increased during storage. The HNE content in samples with NaCl was higher than those of the control, after 7 and 10 days of storage, although the difference was not always significant. Judging from MA content, NaCl may act as a pro-oxidant in pork and beef. HNE may accumulate in pork and beef in which lipid peroxidation is progress. © 2003 Published by Elsevier Ltd.
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Katayama K., Tomatsu M., Kawahara S., Yamauchi K., Fuchu H., Kodama Y., Kawamura Y., Muguruma M.
Journal of Agricultural and Food Chemistry 52 ( 4 ) 771 - 775 2004年2月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Journal of Agricultural and Food Chemistry
A novel angiotensin I-converting enzyme (ACE) inhibitory peptide (RMLGQTPTK; 9mer) from porcine skeletal troponin C was investigated for its inhibitory profile. This peptide was noncompetitive and as hydrophobic as the known ACE inhibitory peptides. Aminopeptidase M quickly hydrolyzed 9mer, resulting in production of MLGQTPTK and LGQTPTK with inhibitory activities similar to those of 9mer. The main hydrolysis product of 9mer with carboxypeptidase A and B was RMLGQTPT showing very weak activity. Most products derived from 9mer hydrolysis by ACE, aminopeptidase, or carboxypeptidase showed weak but definite ACE inhibitory activities. Thus, 9mer was estimated to be a wholly efficient inhibitor with these fragment peptides.
DOI： 10.1021/jf0350865
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河原 聡, 田丸 靜香, 窄野 昌信, 福田 亘博, 池田 郁男
榮養學雑誌 62 ( 1 ) 1 - 7 2004年2月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：日本栄養改善学会
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河原 聡, 田丸 靜香, 窄野 昌信, 福田 亘博, 池田 郁男
栄養学雑誌 62 ( 1 ) 1 - 7 2004年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：The Japanese Society of Nutrition and Dietetics
Conjugated linoleic acid (CLA) is the collective name for a group of geometric and positional isomers of octadecadienoic acid (18: 2) with conjugated double bonds. Interest in CLA has increased in the past decade as a result of its potential beneficial health effects on experimental animals. We summarize in this review the occurrence in food, biological effects and typical human consumption of CLA. We also review the efficacy and some problems of CLA supplementation as a weight loss agent, and the findings from animal studies and <i>in vitro</i> studies. Since CLA is biologically produced by the rumen bacteria of ruminant animals, ruminant meat and milk contain relatively higher amounts of CLA. The predominant isomers in those foods are 9-<i>cis</i>, 11-<i>trans</i> CLA. On the other hand, chemically synthesized CLA products which are commercially available in the US generally contain equal amounts of 9-<i>cis</i>, 11-<i>trans</i> and 10-<i>trans</i>, 12-<i>cis</i> CLA. The CLA isomers have been shown in animal models to protect against chemically induced cancer and atherosclerosis, and to reduce body fat. Although CLA appears to reduce fat mass in rodents such as mice and rats, we need more evidence to clarify the fat-reducing effect and safety of CLA in humans.
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山内 清, 河原 聡
西日本畜産学会報 47 11 - 19 2004年
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Warm Regional Society of Animal Science, Japan
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Itoi S., Kawahara S., Kondo H., Sakai T., Watabe S.
Fish Physiology and Biochemistry 29 ( 3 ) 237 - 244 2003年12月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Fish Physiology and Biochemistry
Carp undergo temperature acclimation of respiratory function by altering mitochondrial ATP synthase (F0F1-ATPase) both quantitatively and qualitatively (Itoi et al. 2003). To address such acclimation temperature-dependent changes of F0F1-ATPase activity, we investigated in this study the correlation between the fatty acid composition and F0F1-ATPase activity in fast muscle of thermally acclimated carp. The quantities of saturated fatty acids of mitochondria from carp acclimated to 10 °C were significantly lower than those of carp acclimated to 30 °C. While mono- and poly-unsaturated fatty acids tended to increase with cold acclimation of carp, the molar concentration of 16:0 aldehyde in mitochondria from the 10 °C-acclimated carp were less than those from the 30 °C-acclimated fish. The specific activities of F0F1 -ATPase in the 10 °C- and 30 °C-acclimated fish mitochondria were calculated to be 167 ± 22 and 56 ± 10 nmol/min · mg mitochondrial protein, respectively, the difference being significant at P < 0.005. Taken together, the increase in F0F1-ATPase activity in fast muscle mitochondria of carp after cold temperature acclimation may be closely related to the increase of unsaturated fatty acids in mitochondria. © 2004 Kluwer Academic Publishers.
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Katayama K., Katayama K., Fuchu H., Fuchu H., Sugiyama M., Sugiyama M., Kawahara S., Yamauchi K., Kawamura Y., Kawamura Y., Muguruma M.
Asian-Australasian Journal of Animal Sciences 16 ( 9 ) 1384 - 1389 2003年9月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Asian-Australasian Journal of Animal Sciences
In order to clarify one of the biological functions of pork, we investigated whether a peptic hydrolysate of denatured porcine crude myosin showed inhibitory activity against angiotensin I-converting enzyme (ACE), which contributed to hypertension. Our results indicated that this hydrolysate showed relatively strong activity, and we therefore attempted to separate the involved peptides, which were considered to be active substances. To isolate these active peptides, the hydrolysate was separated using a solid-phase separation, gel filtration high-performance liquid chromatography (HPLC), and two kinds of reverse phase HPLC. In each stage of separation, many fractions were detected, almost all of which showed ACE inhibitory activity. Thus, we suggested that the activity of the hydrolysate as a whole was a result of the activities of the many individual peptides. Six peaks were distinguished, with yields from 34 to 596 ppm of original crude myosin. In addition to the six peaks, many other active fractions were found throughout the separation steps, strongly suggesting that whole porcine crude myosin itself had ACE inhibitory activity. Moreover, pork as food was considered to function as an ACE inhibitory material in vivo, because pork proteins consist primarily of crude myosin, which included almost all the myofibrillar structural proteins.
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Peptic hydrolysate of porcine crude myosin has many active fractions inhibiting angiotensinⅠ-converting enzyme. 査読あり
K. Katayama, H. Fuchu, M. Sugiyama, S. Kawahara, K. Yamauchi, Y. Kawamura, M. Muguruma
Asian-Aust. J. Anim. Sci. 16 ( 9 ) 1384 - 1389 2003年9月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Changes in mitochondrial fatty acid composition following temperature acclimation of carp and their possible effects on F0F1-ATPase activity. 査読あり
S. Itoi, S. Kawahara, H. Kondo, T. Sakai, S. Watabe
Fish. Physiol. Biochem. 29 ( 3 ) 237 - 244 2003年9月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Dietary conjugated linoleic acid reduces cerebral prostaglandin E<inf>2</inf> in mice
Nakanishi T., Koutoku T., Kawahara S., Murai A., Furuse M.
Neuroscience Letters 341 ( 2 ) 135 - 138 2003年5月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Neuroscience Letters
Conjugated linoleic acids (CLA) are naturally occurring fatty acids that have been recognized to modify prostaglandin (PG) production in specific tissues. So far, no relationship between PG production and CLA has been reported in the brain. Thus, the effects of CLA on cerebral prostaglandin E2 (PGE2) levels and cyclooxygenase (COX)-1 and COX-2 mRNA expression in mice were determined in the present study. Mice given the diet with or without 3% CLA at the age of 9 weeks were mated, reproduced and lactated. Offspring mice given each diet until 25 days and 8 weeks of age were sacrificed and their brains removed. Production of PGE2 was reduced while COX mRNA was quantitatively increased by CLA supplementation. These results imply that brain PGE2 production can be reduced by dietary CLA without inhibition of COX gene expression in mice. © 2003 Elsevier Science Ireland Ltd. All rights reserved.
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Dietary conjugated linoleic acid reduces cerebral prostaglandin E2 in mice. 査読あり
T. Nakanishi, T. Koutoku, S. Kawahara, A. Murai, M. Furuse
Neurosci. Lett. 341 ( 2 ) 135 - 138 2003年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Effect of NaCl on lipid peroxidation-derived aldehyde, 4-hydroxy-2-nonenal formation in minced pork and beef. 査読あり
T. Sakai, D.M.S. Munasinghe, M. Kashimura, K. Sugamoto, S. Kawahara
Meat Sci. 66 ( 4 ) 789 - 792 2003年4月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Katayama K., Katayama K., Katayama K., Fuchu H., Fuchu H., Sakata A., Sakata A., Kawahara S., Yamauchi K., Kawamura Y., Kawamura Y., Muguruma M.
Asian-Australasian Journal of Animal Sciences 16 ( 3 ) 417 - 424 2003年3月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Asian-Australasian Journal of Animal Sciences
Inhibitory activities against angiotensin I-converting enzyme (ACE) of enzymatic hydrolysates of porcine skeletal muscle proteins were investigated. Myosin B, myosin, actin, tropomyosin, troponin and water-soluble proteins extracted from pork loin were digested by eight kinds of proteases, including pepsin, α-chymotrypsin, and trypsin. After digestion, hydrolysates produced from all proteins showed ACE inhibitory activities, and the peptic hydrolysate showed the strongest activity. In the case of myosin B, the molar concentration of peptic hydrolysate required to inhibit 50% of the activity increased gradually as digestion proceeded. The hydrolysates produced by sequential digestion with pepsin and α-chymotrypsin, pepsin and trypsin or pepsin and pancreatin showed weaker activities than those by pepsin alone, suggesting that ACE inhibitory peptides from peptic digestion might lose their active sequences after digestion by the second protease. However, the hydrolysates produced by sequential digestion showed stronger activities than those by α-chymotrypsin, trypsin or pancreatin alone. These results suggested that the hydrolysates of porcine meat were able to show ACE inhibitory activity, even if they were digested in vivo, and that pork might be a useful source of physiologically functional factors.
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AngiotensinⅠ-converting enzyme inhibitory activities of porcine skeletal muscle proteins following enzyme digestion. 査読あり
K. Katayama, H. Fuchu, A. Sakata, S. Kawahara, K. Yamauchi, Y. Kawamura, M. Muguruma
Asian-Aust. J. Anim. Sci. 16 ( 3 ) 417 - 424 2003年3月
記述言語：英語 掲載種別：研究論文（学術雑誌）
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Lipid peroxidation-derived hepatotoxic aldehydes, 4-hydroxy-2E-hexenal in smoked fish meat products
Munasinghe Deepthi Ms, Ichimaru Ken-ichiro, Ryuno Miyo, UEKI NOBUHIKO, MATSUI TAKANAO, SUGAMOTO KAZUHIRO, KAWAHARA SATOSHI, SAKAI TADASHI
Fisheries science : FS 69 ( 1 ) 189 - 194 2003年2月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Japanese Society of Fisheries Science
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KATAYAMA Kazunori, TOMATSU Makoto, FUCHU Hidetaka, SUGIYAMA Masaaki, KAWAHARA Satoshi, YAMAUCHI Kiyoshi, KAWAMURA Yukio, MUGURUMA Michio
Animal science journal 74 ( 1 ) 53 - 58 2003年2月
記述言語：日本語 掲載種別：研究論文（学術雑誌）
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Microbial transglutaminase modifies gel properties of porcine collagen
Erwanto Y., Kawahara S., Katayama K., Takenoyama S., Takenoyama S., Fujino H., Fujino H., Yamauchi K., Morishita T., Morishita T., Kai Y., Kai Y., Watanabe S., Watanabe S., Muguruma M.
Asian-Australasian Journal of Animal Sciences 16 ( 2 ) 269 - 276 2003年2月
記述言語：日本語 掲載種別：研究論文（学術雑誌） 出版者・発行元：Asian-Australasian Journal of Animal Sciences
We studied the gel properties of porcine collagen with microbial transglutaminase (MTGase) as a catalyst. A creep meter was used to measure the mechanical properties of gel. The results showed samples with high concentration of MTGase gelled faster than those with a low concentration of MTGase. The gel strength increased with incubation time and the peaks of breaking strength for 0.1, 0.2 and 0.5% MTGase were obtained at 40, 20 and 10 min incubation time, respectively. According to SDS-PAGE, the MTGase was successfully created a collagen polymer with an increase in molecular weight, whereas no change in formation was shown without MTGase. The sample with 0.5% MTGase began to polymerize after 10 or 20 min incubation at 50°C, and complete polymerization occurred after 40-60 min incubation. Scanning electron microscopic analysis revealed that the gel of porcine collagen in the presence of MTGase produced an extremely well cross-linked network. The differential scanning calorimetric analysis showed the peak thermal transition of porcine collagen gel was at 36°C, and that with MTGase no peak was detected during heating from 20 to 120°C. The melting point of porcine collagen gel could be controlled by MTGase concentration, incubation temperature and protein concentration. Knowledge of the structural and physicochemical properties of porcine collagen gel catalyzed with MTGase could facilitate their use in food products.
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Microbial transglutaminase modifies gel properties of porcine collagen. 査読あり
Y. Erwanto, S. Kawahara, K. Katayama, S. Takenoyama, H. Fujino, K. Yamauchi, T. Morishita, Y. Kai, S. Watanabe, M, Muguruma
Asian-Aust. J. Anim. Sci. 16 ( 2 ) 269 - 276 2003年2月
記述言語：英語 掲載種別：研究論文（学術雑誌）