Papers - AYABE Takanori
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Ayabe T., Takencika H., Onitsnka T., Shibata K., Takenaka O., Uesitgi S., Hamada M.
Enzyme and Protein 49 ( 5-6 ) 305 - 312 1996
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Enzyme and Protein
Adenylate kinase (AK; EC 2.7.4.3, hAK1) catalyzes the reaction: MgATP2- + AMP2- ⇆ MgADP + ADP3-. To elucidate the catalytic and structural roles of threonine residues in human AK, Thr35 and Thr39 mutants were analyzed by steady-state kinetics. The K(m) values of T35P and T35Y were not changed for MgATP2- and AMP2-, and the k(cat) values were decreased by 1/39 compared to those of wild-type AK. Thr35 was suggested to be essential for catalysis. The K(m), values of T39S, T39V, and T39P were increased 5.6- to 59.0-fold for AMP2-; however, the k(cat) values were not reduced. Although the K(m) values of T39F and T39L were unchanged, the k(cat) values were reduced by more than 1/57. Thr39 appears to play an important role in the binding of AMP2- and to be essential for catalysis. As noted above, a hydroxyl group of the Thr residue in human AK appears to be important.
DOI： 10.1159/000468640
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入所及び在宅筋ジストロフィー患者の血清トコフェロール分画 Reviewed
木村恒, 丸山英晴, 綾部貴典, 濱田稔
厚生省精神・神経疾患研究委託費研究報告書 筋ジストロフィーの療養と看護に関する臨床的,社会学的研究平成6年度 Page81 1995.3
Language：Japanese Publishing type：Research paper (scientific journal)
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人工呼吸器からの離脱が困難であった高度肺高血圧を合併した僧帽弁狭窄術後におけるPGE1の効果 Reviewed
鬼塚敏男, 桑原正知, 中村都英, 矢野裕士, 矢野光洋, 綾部貴典, 松崎泰憲, 柴田紘一郎, 関屋 亮, 古賀保範
現代医療 26 3725 - 3728 1994.9
Language：Japanese Publishing type：Research paper (scientific journal)
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血管造影後の感染性仮性、外腸骨動脈瘤破裂の1治験例 Reviewed
桑原正知, 鬼塚敏男, 中村都英, 福元廣次, 篠原立大, 大岐実枝子, 綾部貴典, 中村栄作, 柴田紘一郎, 古賀保範
外科 56 ( 2 ) 203 - 206 1994.5
Language：English Publishing type：Research paper (scientific journal)