Papers - NANASHIMA Atsushi
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Yasutake T., Hidaka S., Tanaka K., Jibiki M., Shibasaki S., Tsuji T., Nanashima A., Sawai T., Yamaguchi H., Nakagoe T., Nagayasu T.
Acta Medica Nagasakiensia 49 ( 1-2 ) 63 - 66 2004.6
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Acta Medica Nagasakiensia
Gastrointestinal stromal tumors (GISTs) are common form of submucosal tumors of the stomach. Treatment of recurrent GISTs had been unsuccessful because of resistance to chemotherapy and radiotherapy. Recently, GISTs were reported to markedly respond to the molecular target agent, imatinib mesylate. We present here a patient with recurrent GIST and c-kit mutation who was successfully treated with imatinib mesylate. A 66-year-old man underwent partial gastrectomy because of GIST. The tumor was 3 cm in size and positive for KIT expression. One year after the excision, spiral computed tomography (CT) scan revealed four intra-peritoneal recurrence lesions measuring 7, 4, 3 and 2 cm in diameter. One week after the CT scan, we started treatment with imatinib mesylate at 400 mg/day, which resulted within two months in 40% decrease in the sum of the longest diameter. The reduction of tumor size continued for more than 6 months. Analysis of the c-kit mutation of the primary tumor revealed the deletion of 18 bases in exon 11 (codon 551-557), while other exons showed no mutation. In this report, we showed the effectiveness of imatinib mesylate therapy for the recurrence of GIST, especially with c-kit mutation in exon 11.
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Taniguchi Y., Tagawa Y., Yasutake T., Nanashima A., Yamasaki N., Nakamura A., Sawai T., Tagawa T., Nagayasu T.
Acta Medica Nagasakiensia 49 ( 1-2 ) 33 - 38 2004.6
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Acta Medica Nagasakiensia
This study was designed to provide quantitative analysis using flow cytometry (FCM) and immunohistochemical analysis (IH) for p53 protein in 85 patients who underwent pulmonary resection for non-small cell lung carcinoma (NSCLC). We also examined the relationship among numerical aberrations of chromosome 17 and p53 locus, clinicopathological parameters and patient prognosis in NSCLC. PAb 1801 was used as the primary antibody for p53 and the Fluorescence Index (F.I.) was calculated by FCM. Fiffy-six patients (66%) showed a higher F.I (≧0.5), and had a higher rate of lymph node metastasis, more advanced stage and poor survival, while a positive expression of p53 protein by IH was associated with no clinicopathologic factors or patient survival. The F.I. of p53 protein was significantly higher in cases with imbalanced numbers between chromosome 17 and the p53 locus, particularly in patients with higher F.I. This indicated that quantitative analysis by FCM was the most useful method to detect the over-expression of p53 protein compared to that by IH. Higher F.I. (≧0.5) is a prognostic indicator for predicting malignant behavior and poor survival in patients with NSCLC.
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Relationship between indocyanine green test and technetium-99m galactosyl serum albumin scintigraphy in patients scheduled for hepatectomy: Clinical evaluation and patient outcome.
Nanashima A, Yamaguchi H, Shibasaki S, Morino S, Ide N, Takeshita H, Sawai T, Nakagoe T, Nagayasu T, Ogawa Y
Hepatology research : the official journal of the Japan Society of Hepatology 28 ( 4 ) 184 - 190 2004.4
Language：Japanese Publishing type：Research paper (scientific journal)
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Japanese Journal of Gastroenterological Surgery 37 ( 1 ) 92 - 97 2004.2
Language：Japanese Publishing type：Case report
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Additional radical surgery after colonoscopic snare polypectomy for T1 colorectal cancer: use of the minilaparotomy approach.
Nakagoe T, Sawai T, Tsuji T, Hidaka S, Tanaka K, Shibasaki S, Nanashima A, Yamaguchi H, Yasutake T
International surgery 89 ( 1 ) 10 - 4 2004.1
Language：Japanese Publishing type：Research paper (scientific journal)
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Numerical aberrations of chromosome 17 and the p53 locus in small hepatocellular carcinomas.
Yano H, Nanashima A, Hidaka S, Haseba M, Tanaka K, Yamaguchi H, Nakagoe T, Tagawa Y, Nagayasu T
Anticancer research 24 ( 1 ) 111 - 5 2004.1
Language：Japanese Publishing type：Research paper (scientific journal)
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Numerical Aberrations of Chromosome 17 and the p53 Locus in Small Hepatocellular Carcinomas
Yano H., Nanashima A., Hidaka S., Haseba M., Tanaka K., Yamaguchi H., Nakagoe T., Tagawa Y., Nagayasu T.
Anticancer Research 24 ( 1 ) 111 - 115 2004.1
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Anticancer Research
To investigate numerical aberrations of chromosome 17 and the p53 locus in early stages of hepatocellular carcinoma (HCC), 12 fresh-frozen specimens of small HCCs (less than 30 mm in size) were examined by dual-color fluorescence in situ hybridization. We used a chromosome 17 alpha-satellite DNA probe and a p53 locus-specific DNA probe. We also performed immunohistochemical analysis for p53 protein in the same cases. Gain of chromosome 17 was the most frequently observed anomaly, present in 58% of cases, and deletion of the p53 locus was observed in 50% of cases. The combination of chromosome 17 gain and p53 locus deletion was observed in 33.3% of cases. However, overexpression of p53 protein was not observed in any specimens. Our results suggest that gain of chromosome 17 and deletion of the p53 locus could represent early genetic events, prior to overexpression of p53 protein due to mutation, in early stage HCC.
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Immunohistochemical analysis of tumor biological factors in hepatocellular carcinoma: relationship to clinicopathological factors and prognosis after hepatic resection.
Nanashima A, Yano H, Yamaguchi H, Tanaka K, Shibasaki S, Sumida Y, Sawai T, Shindou H, Nakagoe T
Journal of gastroenterology 39 ( 2 ) 148 - 54 2004
Language：Japanese Publishing type：Research paper (scientific journal)
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Survival and recurrence after a sphincter-saving resection and abdominoperineal resection for adenocarcinoma of the rectum at or below the peritoneal reflection: a multivariate analysis.
Nakagoe T, Ishikawa H, Sawai T, Tsuji T, Tanaka K, Hidaka S, Nanashima A, Yamaguchi H, Yasutake T
Surgery today 34 ( 1 ) 32 - 9 2004
Language：Japanese Publishing type：Research paper (scientific journal)
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Preoperative serum hyaluronic acid level as a good predictor of posthepatectomy complications.
Nanashima A, Yamaguchi H, Tanaka K, Shibasaki S, Tsuji T, Ide N, Hidaka S, Sawai T, Nakagoe T, Nagayasu T
Surgery today 34 ( 11 ) 913 - 9 2004
Language：Japanese Publishing type：Research paper (scientific journal)
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Minilaparotomy approach for the resection of laterally spreading tumors of the colon.
Nakagoe T, Sawai T, Tsuji T, Tanaka K, Shibasaki S, Hidaka S, Nanashima A, Yamaguchi H, Yasutake T
Surgery today 34 ( 9 ) 737 - 41 2004
Language：Japanese Publishing type：Research paper (scientific journal)
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Minilaparotomy approach for removal of a large colonic lipoma: report of two cases.
Nakagoe T, Sawai T, Tsuji T, Tanaka K, Nanashima A, Shibasaki S, Yamaguchi H, Yasutake T
Surgery today 34 ( 1 ) 72 - 5 2004
Language：Japanese Publishing type：Research paper (scientific journal)
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Nanashima A., Hisamatsu T., Sawai T., Tohyama H., Nakagoe T., Nagayasu T.
Japanese Journal of Gastroenterological Surgery 37 ( 5 ) 614 - 618 2004
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japanese Journal of Gastroenterological Surgery
A 69-year old man was admitted to our hospital with upper abdominal pain caused by the reflux esophagitis. An abdominal CT scan showed a 4cm cystic liver tumor in segment S7/8, and colonoscopy revealed an 8mm early flat-type (IIa + IIc) cancer with central depression and fold convergence in the recto-sigmoid area. Invasive colorectal carcinoma with liver metastasis was suspected, and high anterior resection of the rectum with D2 lymphadenectomy and partial hepatectomy were performed simultaneously. Histological examination revealed the moderately differentiated adenocarcinoma with slight invasion of the submucosal layer (sm1) and venous infiltration, but no lymph node metastasis. The liver tumor with central necrosis showed histological findings similar to those of the primary colorectal carcinoma. The pathological TNM stage according to the system of the Japanese Society for Cancer of the Colon and Rectum was stage IV, and surgical curability was B. We consider this case to be rare and valuable because of its rarity.
DOI： 10.5833/jjgs.37.614
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Increased serum levels of interleukin-6 in malnourished patients with colorectal cancer.
Nakagoe T, Tsuji T, Sawai T, Tanaka K, Hidaka S, Shibasaki Si, Nanashima A, Ohbatake M, Yamaguchi H, Yasutake T, Sugawara K, Inokuchi N, Kamihira S
Cancer letters 202 ( 1 ) 109 - 15 2003.12
Language：Japanese Publishing type：Research paper (scientific journal)
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Increased serum levels of interleukin-6 in malnourished patients with colorectal cancer
Nakagoe T., Tsuji T., Sawai T., Tanaka K., Hidaka S., Shibasaki S., Nanashima A., Ohbatake M., Yamaguchi H., Yasutake T., Sugawara K., Inokuchi N., Kamihira S.
Cancer Letters 202 ( 1 ) 109 - 115 2003.12
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Cancer Letters
Peri-operative serum levels of interleukin-6 (IL-6) were determined in 62 patients who underwent resection of colorectal cancer to clarify the relationship between nutritional status and IL-6 response. Patients were divided into two groups based on creatinine height index: malnourished group (n=13) and normally nourished group (n=49). The preoperative median serum level of IL-6 in the malnourished group was significantly higher than in the normally nourished group (P=0.041). The postoperative median serum level of IL-6 in the malnourished group also tended to be higher. In conclusion, the peri-operative IL-6 response may be activated in malnourished colorectal cancer patients. © 2003 Elsevier Ireland Ltd. All rights reserved.
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Nakagoe T., Ishikawa H., Sawai T., Tsuji T., Hidaka S., Tanaka K., Takeshita H., Ohbatake M., Nanashima A., Akamine S., Yamaguchi H., Yasutake T., Nagayasu T., Kamihira S.
Acta Medica Nagasakiensia 48 ( 3-4 ) 129 - 133 2003.12
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Acta Medica Nagasakiensia
The aim of this study was to clarify whether or not pre-operative serum levels of sialyl Lewis a (CA19-9), sialyl Lewisx (SLX), and sialyl Tn (STN) antigens are predictors for diffuse type gastric cancer. Eighty-two patients with diffuse type and 96 patients with intestinal type cancers were studied. Univariate logistic regression analysis showed that the following factors were significantly associated with diffuse type cancer: high levels of serum STN, young age ( < 62 years), female gender, tumor in the middle stomach, macroscopic type 3/type 4 cancer, presence of lymphatic invasion, peritoneal dissemination, stage III/IV, and non-curative resection. Multivariate analysis revealed that diffuse type cancer was independently related to young age ("62 years), female gender, tumor in the middle stomach, and macroscopic type 3/type 4 cancer. In conclusion, none of the preoperative serum levels of CA19-9, SLX, and STN were predictors for diffuse type cancer.
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Nakagoe T., Yamaguchi E., Tanaka K., Sawai T., Tsuji T., Shibasaki S., Nanashima A., Yamaguchi H., Yasutake T., Ayabe H.
Annals of Surgical Oncology 10 ( 2 ) 163 - 170 2003.12
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Annals of Surgical Oncology
Background: The aim of this study was to clarify the prognostic value of distal intramural spread of tumor for survival and recurrence in patients with rectal cancer. Methods: Microscopic distal intramural spread was examined in 134 consecutive specimens of resected rectal cancer. Correlations among distal intramural spread, established clinicopathologic factors, and patients' prognoses were examined by univariate and multivariate analyses. American Joint Committee on Cancer classification and stage groupings were used for tumor assessment. Results: Thirty-three patients (24.6%) had distal intramural spread. Multivariate logistical regression analysis revealed that T3/T4 and M1 were independent predictive variables for the presence of distal intramural spread. Patients with distal intramural spread had a shorter disease-specific or disease-free survival time after curative surgery than those without distal intramural spread (P = .0003 and P = .0006, respectively). Most patients with distal intramural spread developed distant recurrence. Cox's regression with multiple covariates showed that distal intramural spread is an independent factor in predicting distant recurrence and worse outcomes after curative surgery in patients with rectal cancer. Conclusions: Distal intramural spread is an independent risk factor for distant metastasis and poor prognosis in patients with rectal cancer. © 2003 The Society of Surgical Oncology, Inc.
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Resected or Remnant Liver Volume and Standard Liver Volume Ratio in Patients with Major Hepatectomy
Nanashima A., Yamaguchi H., Shibasaki S., Morino S., Sumida Y., Ide N., Taguchi T., Nakagoe T., Nagayasu T.
Acta Medica Nagasakiensia 48 ( 3-4 ) 125 - 128 2003.12
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Acta Medica Nagasakiensia
To clarify the relationship between resected (RSV) or remnant hepatic volume (RMV) in major hepatectomy, and standard liver volume (SLV) and its clinical significance, the RSV/SLV, RMV/SLV and the volume of regeneration (RGV)/RMV were examined in 41 patients including 19 with chronic hepatitis and 5 with obstructive jaundice who underwent lobectomy or extended lobectomy. The hepatic function was maintained in all patients. SLV was calculated by the body-surface area using Urata's formula. RGV was calculated by subtracting the RMV from the remnant liver volume at day 28 after hemi-hepatectomy. Measurement of the hepatic volume was performed by computed tomography. The means of RSV, RMV, RGV and SLV were 591±173, 459±119, 667±129 and 1128=129cm3, respectively. The means of RSV/SLV, RMV/SLV and RGV/RMV were 0.52±0.14, 0.41±0.12 and 1.54±0.47, respectively. RGV was inversely correlated with RMV/SLV (p<0.001) but not with the other parameters. RSV/SLV and RMV/SLV were not associated with long-term ascites and hepatic failure. The tendency of these results was similar in each patient with a normal liver, obstructive jaundice and chronic viral hepatitis. If the hepatic functional reserve is maintained, a liver with lower hepatic volume has potentially sufficient regeneration even in patients with an injured liver.
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Tsuji T., Hidaka S., Sawai T., Nakagoe T., Yano H., Haseba M., Komatsu H., Shindou H., Fukuoka H., Yoshinaga M., Shibasaki S., Nanashima A., Yamaguchi H., Yasutake T., Tagawa Y.
Clinical Cancer Research 9 ( 10 I ) 3700 - 3704 2003.10
Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Clinical Cancer Research
Thymidylate synthase (TS) is the target enzyme of 5-fluoropyrimidines. The TS gene promoter enhancer region (TSER) possesses tandem, repeated, regulatory sequences that are polymorphic in humans. This polymorphism has been reported to influence TS expression in vitro and in vivo. In this study, we assessed whether or not the TSER genotype is an efficacious marker for tumor sensitivity to 5-fluorouracil (5-FU)-based oral adjuvant chemotherapy for colorectal cancer. One hundred and thirty-five Japanese patients who received curative resection and 5-FU-based oral adjuvant chemotherapy were studied. TSER genotypes of the tumors were analyzed by PCR. The numbers of repeated sequences of representative bands were determined by direct sequence. The genotypes of two-/two-repeats (TSER 2/2), two-/three-repeats (TSER 2/3), three-/ three-repeats (TSER 3/3) and three-/five-repeats (TSER 3/5) were found in 11 (8.1%), 32 (23.7%), 85 (63.0%), and 7 (5.2%) tumors, respectively. Patients were classified into two groups: TSER 2/2 or 2/3 group; and the TSER 3/3 group. The relationship between the TSER genotype group and disease-free intervals was analyzed by univariate and multivariate analyses. Five-year disease-free survivals of the TSER 2/2 or 2/3 group and the TSER 3/3 group were 77% and 75%, respectively (P = 0.89). Multivariate analysis revealed that stage was the only independent prognostic factor and that the TSER genotype did not have a prognostic significance (hazard ratio for TSER 3/3, 0.91; P = 0.84). In conclusion, TSER genotype is not an efficacious marker for tumor sensitivity to 5-FU-based oral adjuvant chemotherapy for Japanese colorectal cancer patients after curative resection.
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Polymorphism in the thymidylate synthase promoter enhancer region is not an efficacious marker for tumor sensitivity to 5-fluorouracil-based oral adjuvant chemotherapy in colorectal cancer.
Tsuji T, Hidaka S, Sawai T, Nakagoe T, Yano H, Haseba M, Komatsu H, Shindou H, Fukuoka H, Yoshinaga M, Shibasaki S, Nanashima A, Yamaguchi H, Yasutake T, Tagawa Y
Clinical cancer research : an official journal of the American Association for Cancer Research 9 ( 10 Pt 1 ) 3700 - 4 2003.9
Language：Japanese Publishing type：Research paper (scientific journal)