Papers - KITAMURA Kazuo
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Yoshibayashi M., Kamiya T., Kitamura K., Saito Y., Kangawa K., Nishikimi T., Matsuoka H., Eto T., Matsuo H.
American Journal of Cardiology 79 ( 11 ) 1556 - 1558 1997.6
Publishing type：Research paper (scientific journal) Publisher：American Journal of Cardiology
To elucidate the pathophysiologic significance of adrenomedullin in pulmonary hypertension, we measured plasma adrenomedullin-like immunoreactivity (AM-LI) concentrations in blood samples obtained from various sites during cardiac catheterization by using radioimmunoassay in patients with pulmonary hypertension in comparison with patients without pulmonary hypertension. In patients with pulmonary hypertension, plasma AM- LI concentrations were significantly elevated and there was a significant uptake of AM-LI in pulmonary circulation, indicating the involvement of adrenomedullin in the cardiovascular regulation of pulmonary circulation in pulmonary hypertension.
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Nishio K., Akai Y., Murao Y., Doi N., Ueda S., Tabuse H., Miyamoto S., Dohi K., Minamino N., Shoji H., Kitamura K., Kangawa K., Matsuo H.
Critical Care Medicine 25 ( 6 ) 953 - 957 1997.6
Publishing type：Research paper (scientific journal) Publisher：Critical Care Medicine
Objective: To investigate plasma concentrations of adrenomedullin in patients with septic shock and the potential association of these concentrations with relaxation of vascular tone. Design: Prospective, case series. Setting: Department of Emergency and Critical Care Medicine, Nara Medical University. Patients: Twelve patients who fulfilled the clinical criteria for severe sepals or septic shock (as defined by the Members of the American College of Chest Physicians/Society of Critical Care Medicine Consensus Conference Committee) and 13 healthy volunteers. Interventions: Arterial blood samples were obtained via a 20-gauge cannula inserted into each patient's radial artery. Measurements and Main Results: After extraction and purification, plasma adrenomedullin was measured by radioimmunoassay. Systemic vascular resistance index, pulmonary vascular resistance, cardiac index, and stroke volume index were determined with a thermodilution catheter. The mean plasma concentration of adrenomedullin was markedly higher in patients than in controls (226.1 ± 66.4 [SEM] va. 5.05 ± 0.21 fmol/mL, p < .01). Moreover, these concentrations correlated significantly with cardiac index, stroke volume index, and heart rate values, and correlated significantly with decreases in diastolic blood pressure, systemic vascular resistance index, and pulmonary vascular resistance index values. Conclusions: Enhanced production of adrenomedullin in patients with septic shock may contribute to reduced vascular tone, hypotension, or both. More data are needed to clarify the role of adrenomedullin in the regulation of vascular tone in this patient population.
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Changes in Cardiac Adrenomedullin Concentration in Renovascular Hypertensive Rats. Reviewed
Ishiyama Yuichiro, Kitamura Kazuo, Kato Johji, Sakata Junichiro, Kangawa Kenji, Eto Tanenao
Hypertension Research 20 ( 2 ) 113 - 117 1997.6
Language：English Publishing type：Research paper (scientific journal) Publisher：The Japanese Society of Hypertension
We assessed changes in tissue and plasma adrenomedullin levels in two-kidney, one-clip renovascular hypertensive rats. Four weeks after clipping, adrenomedullin concentrations were significantly higher in the cardiac ventricles and lower in the left atrium than the respective values in sham-operated rats. The left ventricular adrenomedullin concentration significantly correlated with systolic blood pressure and the degree of cardiac hypertrophy. No difference was noted in the adrenomedullin concentrations of the adrenal gland, aorta, lung, kidneys, or plasma between the two groups. These findings indicate possible involvement of cardiac adrenomedullin in this model of hypertension. (<i>Hypertens Res</i> 1997; 20: 113-117)
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Nishikimi T., Morimoto A., Ishikawa K., Saito Y., Kangawa K., Matsuo H., Kitamura K., Takishita S., Matsuoka H.
Clinical and Experimental Hypertension 19 ( 4 ) 503 - 518 1997.5
Publishing type：Research paper (scientific journal) Publisher：Clinical and Experimental Hypertension
The purpose of this study was to investigate the effect of exercise on plasma concentrations of adrenomedullin, brain natriuretic peptide (BNP), and atrial natriuretic peptide (ANP) in patients with essential hypertension (n = 15) and in normotensive controls (n = 10). Exercise consisted of two fixed workloads, 40 and 80 watts of work load using a supine bicycle ergometer. Plasma levels of all three peptides at rest were significantly higher in hypertensives than in controls. Plasma concentrations of ANP increased with exercise in both groups and had greater increments in hypertensive patients than in normotensives. Plasma concentrations of BNP increased only in patients with hypertension and the levels of increase correlated with basal plasma BNP levels (r = 0.94, p < 0.001) and with left ventricular mass (r = 0.62, p < 0.01) determined by echocardiography. In contrast, plasma adrenomedullin did not change with exercise in either group. These results suggest that secretion patterns of these peptides are regulated by different mechanisms and that the amount and kind of peptides mobilized by exercise may depend on the underlying diseases or pathophysiologic condition.
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Production of adrenomedullin in human vascular endothelial cells Reviewed
Ishihara T., Kato J., Kitamura K., Katoh F., Fujimoto S., Kangawa K., Tanenao E.
Life Sciences 60 ( 20 ) 1763 - 1769 1997.4
Publishing type：Research paper (scientific journal) Publisher：Life Sciences
To examine the production of adrenomedullin (AM) in human vascular endothelial cells, AM concentration in cultured endothelial cells derived from the human umbilical vein and the conditioned media of the cells were measured in the present study. The cultured endothelial cells secreted immunoreactive AM (ir-AM) into the medium at a rate of 14.7 ± 3.0 fmol/106 cells/24 h with an intracellular ir-AM of 5.2 ± 0.8 fmol/106 cells. Analysis by reverse phase high performance-liquid chromatography (HPLC) showed that ir-AM in both the cells and the conditioned medium eluted at the position identical to that of human AM(1-52). Treatment with dexamethasone significantly augmented the secretion of ir-AM from the cells without any effect on the intracellular ir-AM concentration. Northern blot analysis showed not only the presence of the 1.6 kb human AM precursor mRNA in the endothelial cells, but also its increased expression in the dexamethasone-treated cells. Thus, AM was synthesized and secreted by the human endothelial cells of the umbilical vein, and glucocorticoid augmented the AM production. These findings suggest not only the role of AM as a local modulator of the vascular tone but also the possibility that endothelial cells contribute to circulating AM in the human blood.
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Nishikimi T., Nagata S., Sasaki T., Tomimoto S., Matsuoka H., Takishita S., Kitamura K., Miyata A., Matsuo H., Kangawa K.
Heart 78 ( 4 ) 390 - 395 1997
Publishing type：Research paper (scientific journal) Publisher：Heart
Objective - To examine the pathophysiological significance of adrenomedullin in the pulmonary circulation by investigating the relation between plasma concentrations of adrenomedullin and central haemodynamics in patients with mitral stenosis. Methods - Plasma concentrations of adrenomedullin in blood samples obtained from the femoral veins pulmonary artery, left atrium, and aorta were measured by a newly developed specific radio-immunoassay in 23 consecutive patients with mitral stenosis (16 females and seven males, aged 55 (10) years (mean (SD)) who were undergoing percutaneous mitral commissurotomy. Results - Patients with mitral stenosis had higher concentrations of adrenomedullin than age matched normal controls (3.9 (0.3) v 2.5 (0.3) pmol/l, p < 0.001). There was a reduction in adrenomedullin concentrations between the pulmonary artery and the left atrium (3.8 (0.2) v 3.2 (0.4) pmol/l, p < 0.001). The venous concentrations of adrenomedullin correlated with mean pulmonary artery pressure (r = 0.65, p < 0.001), total pulmonary vascular resistance (r = 0.83, p < 0.0001), and pulmonary vascular resistance (r = 0.65, p < 0.001). Plasma concentrations of adrenomedullin did not change immediately after percutaneous mitral commissurotomy; however, they decreased significantly one week later. Conclusions - Plasma concentrations of adrenomedullin are increased in patients with mitral stenosis. This may help to attenuate the increased pulmonary arterial resistance in secondary pulmonary hypertension due to mitral stenosis.
DOI： 10.1136/hrt.78.4.390
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アドレノメデュリン(AM)の多彩な作用 Reviewed
北村和雄
医学のあゆみ 182 ( 2 ) 136 - 137 1997
Publishing type：Research paper (scientific journal)
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Adrenomedullin動態と脳血管障害 Reviewed
竹下啓、高本俊介、小松本悟、奈良昌治、北村和雄
脳卒中 19 ( 6 ) 548 - 548 1997
Publishing type：Research paper (scientific journal)
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心血管系でのアドレノメデュリンとPAMP Reviewed
北村和雄、加藤丈司、鶴田敏博、寒川賢治、江藤胤尚
日本内分泌学会雑誌 73 ( 5 ) 330 - 330 1997
Publishing type：Research paper (scientific journal)
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新しい循環調節ペプチド"アドレノメデュリン"と"PAMP"の発見とその基礎的・臨床的研究 Reviewed
北村和雄
日本内分泌学会雑誌 73 ( 2 ) 63 - 63 1997
Authorship：Lead author Publishing type：Research paper (scientific journal)
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アドレノメデュリン Reviewed
北村和雄
心臓 29 ( 4 ) 281 - 289 1997
Authorship：Lead author Publishing type：Research paper (scientific journal)
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アドレノメデュリンとPAMP Reviewed
北村和雄、江藤胤尚
日本臨牀 55 ( 8 ) 1963 - 1970 1997
Authorship：Lead author Publishing type：Research paper (scientific journal)
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起立性低血圧を認める糖尿病性神経障害患者と健常者におけるティルティング負荷に対する血中アドレノメデュリン(AM)動態 Reviewed
中村
日本内分泌学会雑誌 73 ( 2 ) 280 - 280 1997
Publishing type：Research paper (scientific journal)
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覚醒ラットにおけるアドレノメデュンの末梢および中枢作用について Reviewed
斉田光彦、下川歩、國武孝人、加藤和男、白阪哲朗、北村和雄、江藤胤尚、河南洋
日本内分泌学会雑誌 73 ( 5 ) 355 - 355 1997
Publishing type：Research paper (scientific journal)
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アドレノメデュリン Reviewed
杉田江里、谷山松雄、伴良雄、北村和雄
病理と臨床 15 ( 3 ) 253 - 258 1997
Publishing type：Research paper (scientific journal)
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本態性高血圧症に対する塩酸ベニジピン(コニール(R))長期投与の検討-宮崎コニール研究会- Reviewed
江藤胤尚、木田修、北村和雄、児玉健二、近藤宏一、比嘉利信、熊谷治士、田口利文、市来能成、石山雄一郎
臨牀と研究 78 ( 8 ) 2079 - 2086 1997
Publishing type：Research paper (scientific journal)
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長期持続静注アドレノメデュリン(AM)の降圧効果-無麻酔無拘束の高血圧自然発症ラット(SHR)およびWistar-Kyotoラット(WKY)を用いた検討- Reviewed
加藤丈司、カーンIアズハルル、北村和雄、寒川賢治、江藤胤尚
Therapeutic Research 18 ( 7 ) 2201 - 2204 1997
Publishing type：Research paper (scientific journal)
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呼吸器疾患における血漿アドレノメデュリンおよびPAMP濃度に関する検討 Reviewed
下窪徹、比嘉利信、田中雅之、石畠英昭、鬼塚黎子
臨牀と研究 74 ( 10 ) 2507 - 2510 1997
Publishing type：Research paper (scientific journal)
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悪性褐色細胞腫多発性肝転移例に対する, 経肝動脈塞栓療法におけるアドレノメデユリンの動態 Reviewed
芦澤直人、高橋和宏、馬場是明、鈴木伸、北村和雄、江藤胤尚、矢野捷介
日本内分泌学会雑誌 73 ( 5 ) 355 - 355 1997
Publishing type：Research paper (scientific journal)
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Cardiac secretion of adrenomedullin by percutaneous transluminal coronary angioplasty Reviewed
Etoh T., Doi H., Hanada Y., Kato J., Imamura T., Kitamura K., Koiwaya Y., Eto T.
Hormone and Metabolic Research 29 ( 10 ) 530 - 531 1997
Publishing type：Research paper (scientific journal) Publisher：Hormone and Metabolic Research